Name: Salipaludibacillus agaradhaerens PN 105
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 1120

Type strain:

Species: Salipaludibacillus agaradhaerens

Strain Designation: PN 105, AC 13

Culture col. no.: DSM 8721, ATCC 700163, CIP 105302

Strain history: CIP <- 1997, DSMZ <- H. Outtrup, Novo Disc A/S: strain PN 105

NCBI tax ID(s): 76935 (species)

SI-ID 10538

LMG 17948, DSM 8721, ATCC 700163, CIP 105302, KCTC 3964, NRRL B-23345, CGMCC 1.3410

Special Collections

Other strains from Salipaludibacillus agaradhaerens

Section

Salipaludibacillus agaradhaerens PN 105 is a facultative anaerobe, spore-forming, Gram-positive bacterium of the family Bacillaceae.

Gram-positive
motile
rod-shaped
spore-forming
facultative anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Salipaludibacillus*
	Species	**Salipaludibacillus agaradhaerens**
	Full Scientific Name (LPSN)	Salipaludibacillus agaradhaerens (Nielsen et al. 1995) Sultanpuram and Mothe 2016

Synonym

Bacillus agaradhaerens

Information on morphological and physiological properties Morphology

[Ref.: #43624]	Gram stain	positive
[Ref.: #116145]	Gram stain	positive

[Ref.: #43624]	Cell shape	rod-shaped
[Ref.: #116145]	Cell shape	rod-shaped

[Ref.: #43624]	Motility	yes
[Ref.: #116145]	Motility	no

[Ref.: #43624]

Colony color

White

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3251]

Culture medium

ALKALINE NUTRIENT AGAR (DSMZ Medium 31)

[Ref.: #3251]

Culture medium growth

[Ref.: #3251]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3251]

Culture medium composition

expand / minimize

[Ref.: #41880]

Culture medium

MEDIUM 113 - for Bacillus

[Ref.: #41880]

Culture medium growth

[Ref.: #41880]

Culture medium composition

expand / minimize

[Ref.: #116145]

Culture medium

CIP Medium 113

[Ref.: #116145]

Culture medium growth

[Ref.: #116145]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #3251]		growth	30 °C
[Ref.: #41880]		growth	30 °C
[Ref.: #43624]		growth	10-45 °C
[Ref.: #43624]		optimum	37 °C
[Ref.: #116145]		growth	10-45 °C
[Ref.: #116145]	no	growth	55 °C

	Kind of pH		pH
[Ref.: #43624]		optimum	8.5-9
[Ref.: #43624]		growth	7.5-9.7
[Ref.: #116145]	no	growth	6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43624]	Oxygen tolerance	facultative anaerobe
[Ref.: #116145]	Oxygen tolerance	obligate aerobe

[Ref.: #43624]	Spore description	oval or ellipsoidal endospores at the sub-terminal position
[Ref.: #43624]	Type of spore	endospore
[Ref.: #43624]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %92.135
[Ref.: #69481]	Ability of spore formation	yes Confidence in %99

	Salt		Salt conc.
[Ref.: #43624]	NaCl	growth	0-15	%(w/v)
[Ref.: #43624]	NaCl	optimum	2-3	%(w/v)
[Ref.: #116145]	NaCl	growth	0-10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43624]	casein	-	hydrolysis
[Ref.: #43624]	cellulose ChEBI	-	hydrolysis
[Ref.: #43624]	citrate ChEBI	-	assimilation
[Ref.: #43624]	dna ChEBI	-	hydrolysis
[Ref.: #43624]	esculin ChEBI	-	hydrolysis
[Ref.: #43624]	gelatin ChEBI	-	hydrolysis
[Ref.: #43624]	glucose ChEBI	-	fermentation
[Ref.: #43624]	nitrate ChEBI	+	reduction
[Ref.: #116145]	nitrate ChEBI	+	reduction
[Ref.: #116145]	nitrate ChEBI	+	respiration
[Ref.: #116145]	nitrite ChEBI	-	reduction
[Ref.: #43624]	starch ChEBI	-	hydrolysis
[Ref.: #43624]	tryptophan ChEBI	-	energy source
[Ref.: #43624]	tween 20 ChEBI	+	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #43624]	acetoin ChEBI	no
[Ref.: #43624]	hydrogen sulfide ChEBI	no
[Ref.: #43624]	indole ChEBI	no
[Ref.: #116145]	indole ChEBI	no

	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test
[Ref.: #43624]	acetoin ChEBI	-
[Ref.: #116145]	acetoin ChEBI	+
[Ref.: #43624]	glucose ChEBI		-
[Ref.: #116145]	glucose ChEBI		+
[Ref.: #43624]	indole ChEBI			-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #116145]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #116145]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #43624]	catalase	+	1.11.1.6
[Ref.: #116145]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #43624]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #116145]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #116145]	gelatinase	+
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #116145]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #116145]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #116145]	oxidase	-
[Ref.: #116145]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #43624]	tryptophan deaminase	-	4.1.99.1
[Ref.: #43624]	urease	-	3.5.1.5
[Ref.: #116145]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116145]	8599	-	-	+	+	-	-	-	-	-	+	-	+	-	+	-	-	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #69479]

Global distribution of 16S sequence X76445 (>99% sequence identity) for Salipaludibacillus agaradhaerens subclade from Microbeatlas

Aquatic Samples	54
Soil Samples	22
Animal Samples	29
Plant Samples
Total Samples	105

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3251]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116145]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #3251]	Bacillus agaradhaerens DSM 8721, 16S rRNA gene	X76445	1521	GenBank ENA	76935 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Salipaludibacillus agaradhaerens DSM 8721	GCA_002019735	scaffold	GenBank ENA	76935 tax ID	*
[Ref.: #66792]	[Bacillus] agaradhaerens strain DSM 8721	76935.4	wgs	PATRIC	76935 tax ID	*
[Ref.: #66792]	Salipaludibacillus agaradhaerens DSM 8721	2890278133	draft	JGI IMG/M	76935 tax ID	*

[Ref.: #3251]	GC-content	39.2 mol%	high performance liquid chromatography (HPLC)
[Ref.: #43624]	GC-content	39.3-39.5 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Salipaludibacillus agaradhaerens DSM 8721 NCBI: GCA_002019735.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	99	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Salipaludibacillus agaradhaerens DSM 8721 NCBI: GCA_002019735.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	80.974	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	92.691	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	62.222	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	92.135	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	91.948	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	77.715	no

Availability in culture collections External links

[Ref.: #3251]

Culture collection no.

DSM 8721, ATCC 700163, CIP 105302

[Ref.: #70791]

SI-ID 10538

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Salipaludibacillus halalkaliphilus sp. nov., a moderately haloalkaliphilic bacterium from a coastal-marine wetland.	Amoozegar MA, Shahinpei A, Makzum S, Rafieyan S, Moshtaghi Nikou M, Sproer C, Ventosa A	Int J Syst Evol Microbiol	10.1099/ijsem.0.002814	2018	*
Phylogeny	Salisediminibacterium halotolerans gen. nov., sp. nov., a halophilic bacterium from soda lake sediment.	Jiang F, Cao SJ, Li ZH, Fan H, Li HF, Liu WJ, Yuan HL	Int J Syst Evol Microbiol	10.1099/ijs.0.034488-0	2011	*
Phylogeny	Bacillus locisalis sp. nov., a new haloalkaliphilic species from hypersaline and alkaline lakes of China, Kenya and Tanzania.	Marquez MC, Carrasco IJ, de la Haba RR, Jones BE, Grant WD, Ventosa A	Syst Appl Microbiol	10.1016/j.syapm.2011.04.003	2011	*
Phylogeny	Bacillus taeanensis sp. nov., a halophilic Gram-positive bacterium from a solar saltern in Korea.	Lim JM, Jeon CO, Kim CJ	Int J Syst Evol Microbiol	10.1099/ijs.0.64036-0	2006	*
Enzymology	Salt-activated endoglucanase of a strain of alkaliphilic Bacillus agaradhaerens.	Hirasawa K, Uchimura K, Kashiwa M, Grant WD, Ito S, Kobayashi T, Horikoshi K	Antonie Van Leeuwenhoek	10.1007/s10482-005-9023-0	2006	*
Enzymology	Sequence analysis of cyclodextrin glycosyltransferase from the alkaliphilic Bacillus agaradhaerens.	Martins RF, Delgado O, Hatti-Kaul R	Biotechnol Lett	10.1023/a:1025430532333	2003	*

References

#3251

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 8721

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#41880 ; Curators of the CIP;

#43624

Vishnuvardhan Reddy Sultanpuram, Thirumala Mothe: Salipaludibacillus aurantiacus gen. nov., sp. nov. a novel alkali tolerant bacterium, reclassification of Bacillus agaradhaerens as Salipaludibacillus agaradhaerens comb. nov. and Bacillus neizhouensis as Salipaludibacillus neizhouensis comb. nov.. IJSEM 66: 2747 - 2753 2016 ( DOI 10.1099/ijsem.0.001117 , PubMed 27118186 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#70791

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID10538.1 )

#116145 Collection of Institut Pasteur ; Curators of the CIP; CIP 105302
* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Salipaludibacillus agaradhaerens in external resources:
SILVA
BRENDA
PANGAEA
GBIF

Salipaludibacillus agaradhaerens DSM 8721

Salipaludibacillus agaradhaerens DSM 8721

Change proposal

Change proposal