Gordonia sputi (DSM 43346, CMI 347, IMI 347)

Name: Gordonia sputi (DSM 43346, CMI 347, IMI 347)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 10569

Type strain

Strain Designation N 647

Culture col. no. DSM 43346 CMI 347 IMI 347 N 647 CIP 104633 1 more

NCBI tax ID(s) 36823

Special Collections DSMZ CIP

History DSMZ <- M. Goodfellow, N 647 (Nocardia kirovani) <- G. Michel, IM 1347 CIP <- 1995, PCM <- IMET <- M. Goodfellow: strain N647, Nocardia kirovani <- G. Michel: strain IMI 347

Links

Gordonia sputi N 647 is an obligate aerobe, Gram-positive, rod-shaped bacterium of the family Gordoniaceae.

Gram-positive rod-shaped obligate aerobe Bacteria

Name and taxonomic classification

SI-ID 50248

CIP 104633,DSM 43346

@ref 20215

Last LPSN update 2026-05-29 08:27:21

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Mycobacteriales

Family Gordoniaceae

Genus Gordonia

Species Gordonia sputi

Full scientific name Gordonia sputi corrig. (Tsukamura and Yano 1985 ex Tsukamura 1978) Stackebrandt et al. 1989

Synonyms (2)

Gordona sputi
Rhodococcus sputi

BacDive ID	Other strains from **Gordonia sputi** (16)	Type strain
10570	G. sputi 3884, DSM 43896, ATCC 29627, IMET 7569, JCM ... (type strain)
10568	G. sputi S672, DSM 43022, KCC A-0019, NRRL B-1531, ...
10571	G. sputi N935, DSM 43979, ATCC 33610, JCM 6048, CIP ...
10572	G. sputi E6324, DSM 44019, ATCC 33609, JCM 6047, CIP ...
10573	G. sputi S681, 495, Hirsch 495, DSM 46016, IMET 7173, ...
10574	G. sputi 6130, Gdf N931, N 931, DSM 46087, CIP 104651, ...
10628	G. sputi Georgia B-1048, N53, DSM 43007
147222	G. sputi CCUG 33089
147781	G. sputi CCUG 34215
148429	G. sputi CCUG 35424
148580	G. sputi CCUG 35666
148813	G. sputi CCUG 35998
151976	G. sputi CCUG 45412
154388	G. sputi CCUG 53357
155392	G. sputi CCUG 57593
165399	G. sputi JCM 6411

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
122295	positive	rod-shaped

Multimedia

@ref:	10970
multimedia content:	DSM_43346.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_43346.jpg
caption:	Medium 535 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
10970	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
10970	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
41256	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
122295	CIP Medium 3	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
10970	positive	growth	28
41256	positive	growth	30
122295	positive	growth	25-37
122295	negative	growth	10
122295	negative	growth	41
122295	negative	growth	45

Physiology and metabolism

Oxygen tolerance

122295

Oxygen toleranceobligate aerobe

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
122295	16947 ChEBI	citrate	-	carbon source
122295	4853 ChEBI	esculin	+	hydrolysis
122295	17632 ChEBI	nitrate	+	reduction
122295	17632 ChEBI	nitrate	-	respiration
122295	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
122295	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
122295	15688 ChEBI	acetoin	-
122295	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
122295	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
122295	amylase	-
68382	beta-galactosidase	+	3.2.1.23	from API zym
122295	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
122295	caseinase	-	3.4.21.50
122295	catalase	+	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
122295	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
122295	gamma-glutamyltransferase	-	2.3.2.2
122295	gelatinase	-
122295	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
122295	lipase	-
68382	lipase (C 14)	+		from API zym
122295	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
122295	ornithine decarboxylase	-	4.1.1.17
122295	oxidase	-
122295	protease	-
68382	trypsin	+	3.4.21.4	from API zym
122295	tryptophan deaminase	-
122295	tween esterase	-
122295	urease	+	3.5.1.5
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
122295	-	+	+	+	+	+	+	+	+	-	+	+	+	+	-	+	+	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
122295	not determinedn.d.	+	+	-	+	+	-	-	+	-	+	+	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	+	-	-	-	-	+	-	+	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	+	-	-	-	-	-	-	+	-	-	+	+	-	-	-	-	-	-	-	-	-	+	-	-	-	-	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
10970	2	Risk group (German classification) According to TRBA 466
122295	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Proteome	Detection and accurate identification of Mycobacterium species by flow injection tandem mass spectrometry (FIA-MS/MS) analysis of mycolic acids.	Rafal S, Magdalena D, Karol M, Bartlomiej S, Konrad K.	Sci Rep	10.1038/s41598-025-96867-x	2025

References

#10970	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43346
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#41256	; Curators of the CIP;
#68382	Automatically annotated from API zym .
#122295	Collection of Institut Pasteur ; Curators of the CIP; CIP 104633
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

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Gordonia sputi (DSM 43346, CMI 347, IMI 347)

Name and taxonomic classification

Morphology

Cell morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API biotype100

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

Sequence information

Genome-based predictions

Literature

Literature

References

BacDive

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