Name: Neisseria gonorrhoeae B5025
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 10470

Type strain:

Species: Neisseria gonorrhoeae

Strain Designation: B5025, B 5025

Culture col. no.: CCUG 26876, CIP 79.18, DSM 9188, ATCC 19424, NCTC 8375, JCM 33543

Strain history: CIP <- 1979, ATCC <- NCTC <- J.F. Wilson, UK: strain B 5025

NCBI tax ID(s): 485 (species)

SI-ID 389413

ATCC 19424, NCTC 8375, CCUG 26876, DSM 9188, CIP 79.18, KCTC 2746, ACM 4989

Special Collections

Other strains from Neisseria gonorrhoeae

Section

Neisseria gonorrhoeae B5025 is a microaerophile, Gram-negative bacterium of the family Neisseriaceae.

Gram-negative
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Neisseriales*
	Family	*Neisseriaceae*
	Genus	*Neisseria*
	Species	**Neisseria gonorrhoeae**
	Full Scientific Name (LPSN)	Neisseria gonorrhoeae (Zopf 1885) Trevisan 1885 (Approved Lists 1980)

Synonym

"Merismopedia gonorrhoeae"

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.083

[Ref.: #3507]	Type of hemolysis	gamma
[Ref.: #3507]	Incubation period	1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3507]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #3507]

Culture medium growth

[Ref.: #3507]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3507]

Culture medium composition

expand / minimize

[Ref.: #36032]

Culture medium

MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and Neisseria

[Ref.: #36032]

Culture medium growth

[Ref.: #36032]

Culture medium composition

expand / minimize

[Ref.: #3507]

Culture medium

CHOCOLATE AGAR (DSMZ Medium 429b)

[Ref.: #3507]

Culture medium growth

[Ref.: #3507]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3507]

Culture medium composition

expand / minimize

[Ref.: #119705]

Culture medium

CIP Medium 10

[Ref.: #119705]

Culture medium growth

[Ref.: #119705]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #3507]	growth	37 °C
[Ref.: #36032]	growth	37 °C
[Ref.: #48585]	growth	37 °C
[Ref.: #67770]	growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #3507]	Oxygen tolerance	microaerophile
[Ref.: #48585]	Oxygen tolerance	microaerophile

[Ref.: #69480]

Ability of spore formation

no Confidence in %92.411

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68377]	D-fructose ChEBI	-	builds acid from	* from API NH
[Ref.: #68377]	maltose ChEBI	-	builds acid from	* from API NH
[Ref.: #68377]	ornithine ChEBI	-	degradation	* from API NH
[Ref.: #68377]	sucrose ChEBI	-	builds acid from	* from API NH
[Ref.: #68377]	tryptophan ChEBI	-	energy source	* from API NH

	Metabolite production	Metabolite	Production
[Ref.: #68377]		indole ChEBI	no	* from API NH

	Physiological tests	Metabolite	Indole test
[Ref.: #68377]		indole ChEBI	-	* from API NH

	Enzyme	Enzyme activity	EC number
[Ref.: #3507]	catalase	+	1.11.1.6
[Ref.: #3507]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #68377]	ornithine decarboxylase	-	4.1.1.17	* from API NH
[Ref.: #68377]	tryptophan deaminase	-	4.1.99.1	* from API NH

API® NH:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Penicillinase productionPEN	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from maltoseMAL	Acid from sucroseSAC	Ornithine decarboxylaseODC	Urease/urea hydrolysisURE	LipaseLIP	Alkaline phosphatasePAL	beta-Galactosidasebeta GAL	Proline arylamidaseProA	gamma-Glutamyl transferaseGGT	Indole productionIND
[Ref.: #3507]	6251	-	+	-	-	-	-	-	-	-	-	+	-	-
[Ref.: #3507]	5842	-	-	-	-	-	-	-	-	-	-	+	-	-
[Ref.: #3507]	5843	-	+	-	-	-	-	-	+/-	+/-	-	+	-	-
[Ref.: #3507]	5844	-	+	-	-	-	-	-	-	-	-	+	-	-
[Ref.: #3507]	5845	-	+	-	-	-	-	-	-	-	-	+	+	-
[Ref.: #3507]	5922	-	+	-	-	-	-	-	-	-	-	+	-	-
[Ref.: #3507]	6028	+	-	-	-	-	-	-	-	-	+	+	-	-
[Ref.: #3507]	6029	+	-	-	-	-	-	+/-	+/-	-	+	-	-	-
[Ref.: #3507]	6030	-	-	-	-	-	-	-	-	-	+	-	-	-
[Ref.: #3507]	6031	+	-	-	-	-	-	-	-	-	+	-	-	-
[Ref.: #3507]	6284	-	+	-	-	-	-	-	-	-	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #48585]	Sampling date	1950
[Ref.: #48585]	Geographic location (country and/or sea, region)	Sunderland
[Ref.: #48585]	Country	United Kingdom
[Ref.: #48585]	Country ISO 3 Code	GBR
[Ref.: #48585]	Continent	Europe

[Ref.: #36032]	Geographic location (country and/or sea, region)	Sunderland
[Ref.: #36032]	Country	United Kingdom
[Ref.: #36032]	Country ISO 3 Code	GBR
[Ref.: #36032]	Continent	Europe

[Ref.: #3507]	Geographic location (country and/or sea, region)	Sunderland
[Ref.: #3507]	Country	United Kingdom
[Ref.: #3507]	Country ISO 3 Code	GBR
[Ref.: #3507]	Continent	Europe
[Ref.: #3507]	Geographic location	54.9000°/-1.3833°

[Ref.: #119705]	Geographic location (country and/or sea, region)	Sunderland
[Ref.: #119705]	Country	United Kingdom
[Ref.: #119705]	Country ISO 3 Code	GBR
[Ref.: #119705]	Continent	Europe
[Ref.: #119705]	Isolation date	1950
* marker position based on {}

[Ref.: #69479]

Global distribution of 16S sequence X07714 (>99% sequence identity) for Neisseria gonorrhoeae subclade from Microbeatlas

Aquatic Samples	208
Soil Samples	26
Animal Samples	3447
Plant Samples	13
Total Samples	3694

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3507]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #119705]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #3507]	Neisseria gonorrhoeae 16S rRNA gene, partial	AJ247238	457	GenBank ENA	485 tax ID
[Ref.: #20218]	Neisseria gonorrhoeae gene for 16S ribosomal RNA	X07714	1544	GenBank ENA	485 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Neisseria gonorrhoeae DSM 9188	GCA_003315235	scaffold	GenBank ENA	485 tax ID
[Ref.: #66792]	Neisseria gonorrhoeae strain DSM 9188	485.1339	wgs	PATRIC	485 tax ID	*
[Ref.: #66792]	Neisseria gonorrhoeae DSM 9188	2770939566	draft	JGI IMG/M	485 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Neisseria gonorrhoeae strain DSM 9188 PATRIC: 485.1339
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	99.083	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	96.409	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	92.411	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	60.51	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	98	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	88.316	no

Availability in culture collections External links

[Ref.: #3507]

Culture collection no.

CCUG 26876, CIP 79.18, DSM 9188, ATCC 19424, NCTC 8375, JCM 33543

[Ref.: #79747]

SI-ID 389413

Literature:

Only first 10 entries are displayed. Click here to see all.

	Title	Authors	Journal	DOI	Year
Pathogenicity	Plants traditionally used individually and in combination to treat sexually transmitted infections in northern Maputaland, South Africa: antimicrobial activity and cytotoxicity.	Naidoo D, van Vuuren SF, van Zyl RL, de Wet H	J Ethnopharmacol	10.1016/j.jep.2013.07.018	2013	*
Metabolism	Decreased affinity of mosaic-structure recombinant penicillin-binding protein 2 for oral cephalosporins in Neisseria gonorrhoeae.	Ochiai S, Sekiguchi S, Hayashi A, Shimadzu M, Ishiko H, Matsushima-Nishiwaki R, Kozawa O, Yasuda M, Deguchi T	J Antimicrob Chemother	10.1093/jac/dkm166	2007	*
Pathogenicity	Mosaic-like structure of penicillin-binding protein 2 Gene (penA) in clinical isolates of Neisseria gonorrhoeae with reduced susceptibility to cefixime.	Ameyama S, Onodera S, Takahata M, Minami S, Maki N, Endo K, Goto H, Suzuki H, Oishi Y	Antimicrob Agents Chemother	10.1128/AAC.46.12.3744-3749.2002	2002	*
Pathogenicity	Evaluation of Gonozyme, an enzyme immunoassay for the rapid diagnosis of gonorrhea.	Manis RD Jr, Harris B, Geiseler PJ	J Clin Microbiol	10.1128/jcm.20.4.742-746.1984	1984	*
Phylogeny	Kingella pumchi sp. nov., an organism isolated from human vertebral puncture tissue.	Xiao M, Liu R, Du J, Liu R, Zhai L, Wang H, Yao S, Xu YC	Antonie Van Leeuwenhoek	10.1007/s10482-022-01786-w	2022	*
	Biological activities and variation of symbiotic fungi isolated from Coral reefs collected from Red Sea in Egypt.	Abd El-Rahman TMA, Tharwat NA, Abo El-Souad SMS, El-Beih AA, El-Diwany AI	Mycology	10.1080/21501203.2020.1741470	2020	*

References

#3507

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 9188

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#36032 ; Curators of the CIP;
#48585 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 26876

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68377

Automatically annotated from API NH .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#79747

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID389413.1 )

#119705 Collection of Institut Pasteur ; Curators of the CIP; CIP 79.18
* These data were automatically processed and therefore are not curated

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Neisseria gonorrhoeae strain DSM 9188

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