Strain identifier
BacDive ID: 8625
Type strain: 
Species: Mesomycoplasma lagogenitalium
Strain Designation: 12MS
Strain history: CIP <- 1998, H. Kobayashi, National Inst. Animal Health, Ibaraki, Japan: strain 12MS
NCBI tax ID(s): 171286 (species)
version 8.1 (current version)
General
@ref: 16049
BacDive-ID: 8625
DSM-Number: 22062
keywords: genome sequence, 16S sequence, Bacteria, mesophilic
description: Mesomycoplasma lagogenitalium 12MS is a mesophilic bacterium that was isolated from preputial smegma of Afghan pikas Ochotona rufescens rufescens.
NCBI tax id
- NCBI tax id: 171286
- Matching level: species
strain history
| @ref | history |
|---|---|
| 16049 | <- CIP <- H. Kobayashi, Natl. Inst. Animal Health, Tsukuba, Ibaraki, Japan; 12MS |
| 32730 | 1998, H. Kobayashi, Nat. Inst. Animal Health, Ibaraki, Japan: strain 12MS |
| 120548 | CIP <- 1998, H. Kobayashi, National Inst. Animal Health, Ibaraki, Japan: strain 12MS |
doi: 10.13145/bacdive8625.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/mycoplasmatota
- domain: Bacteria
- phylum: Mycoplasmatota
- class: Mollicutes
- order: Mycoplasmoidales
- family: Metamycoplasmataceae
- genus: Mesomycoplasma
- species: Mesomycoplasma lagogenitalium
- full scientific name: Mesomycoplasma lagogenitalium (Kobayashi et al. 1997) Gupta et al. 2018
synonyms
- @ref: 20215
- synonym: Mycoplasma lagogenitalium
@ref: 16049
domain: Bacteria
phylum: Tenericutes
class: Mollicutes
order: Mycoplasmatales
family: Metamycoplasmataceae
genus: Mesomycoplasma
species: Mesomycoplasma lagogenitalium
full scientific name: Mesomycoplasma lagogenitalium (Kobayashi et al. 1997) Gupta et al. 2018
strain designation: 12MS
type strain: yes
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 16049 | SP4-Z MEDIUM (DSMZ Medium 1076b) | yes | https://mediadive.dsmz.de/medium/1076b | Name: SP4-Z MEDIUM (DSMZ Medium 1076b) Composition: Agar 10.0 g/l Tryptone 10.0 g/l Bacto peptone 5.0 g/l PPLO broth 3.5 g/l Urea 2.0 g/l Glucose 1.0 g/l None 1.0 g/l Yeast extract 0.4 g/l DNA 0.2 g/l CMRL 1066 Fetal bovine serum Swine serum Distilled water |
| 32730 | MEDIUM 88 - for Mycoplasma and Acholeplasma | yes | Distilled water make up to (670.000 ml);PPLO broth (17.000 g);Sterile supplement - M00273 (210.000 ml);Yeast extract solution 25 % - M00263 (100.000 ml);0.1 % Phenol red - M00387 (20.000 ml) | |
| 120548 | CIP Medium 88 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=88 |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 16049 | positive | growth | 37 | mesophilic |
| 32730 | positive | growth | 37 | mesophilic |
Isolation, sampling and environmental information
isolation
| @ref | sample type | host species | country | origin.country | continent | isolation date |
|---|---|---|---|---|---|---|
| 16049 | preputial smegma of Afghan pikas Ochotona rufescens rufescens | Ochotona rufescens rufescens | Japan | JPN | Asia | |
| 120548 | Afghan pika, Ochotona rufescens rufescens | Japan | JPN | Asia | 1991 |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Mammals | #Rodentia (Other) |
| #Host Body-Site | #Urogenital tract | |
| #Host Body Product | #Fluids |
taxonmaps
- @ref: 69479
- File name: preview.99_134403.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15820;96_3444;97_67126;98_89731;99_134403&stattab=map
- Last taxonomy: Mycoplasma lagogenitalium subclade
- 16S sequence: AF412983
- Sequence Identity:
- Total samples: 99
- soil counts: 2
- animal counts: 97
Safety information
risk assessment
| @ref | biosafety level | biosafety level comment |
|---|---|---|
| 16049 | 1 | Risk group (German classification) |
| 120548 | 2 | Risk group (French classification) |
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Mycoplasma lagogenitalium strain ATCC 700289 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence | AY785379 | 947 | ena | 171286 |
| 16049 | Mycoplasma lagogenitalium 16S ribosomal RNA gene, partial sequence | AF412983 | 1471 | ena | 171286 |
Genome sequences
- @ref: 66792
- description: Mesomycoplasma lagogenitalium 12MS
- accession: GCA_029854295
- assembly level: complete
- database: ncbi
- NCBI tax ID: 171286
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | no | 93.354 | no |
| flagellated | no | 96.551 | no |
| gram-positive | no | 52.369 | no |
| anaerobic | no | 93.865 | no |
| aerobic | no | 92.263 | no |
| halophile | no | 56.668 | no |
| spore-forming | no | 97.914 | no |
| glucose-ferment | yes | 81.698 | no |
| thermophile | no | 96.43 | yes |
| glucose-util | yes | 90.461 | no |
External links
@ref: 16049
culture collection no.: DSM 22062, ATCC 700289, CIP 105489
straininfo link
- @ref: 78006
- straininfo: 46956
literature
- topic: Metabolism
- Pubmed-ID: 9336930
- title: Mycoplasma lagogenitalium sp. nov., from the preputial smegma of Afghan pikas (Ochotona rufescens rufescens).
- authors: Kobayashi H, Runge M, Schmidt R, Kubo M, Yamamoto K, Kirchhoff H
- journal: Int J Syst Bacteriol
- DOI: 10.1099/00207713-47-4-1208
- year: 1997
- mesh: Animals, Animals, Laboratory/microbiology, Base Composition, Cholesterol/metabolism, Female, Lagomorpha/microbiology, Male, Microscopy, Electron, Mycoplasma/chemistry/*classification/immunology/metabolism/ultrastructure, Mycoplasma Infections/*veterinary, Smegma/*microbiology, Sterols/metabolism, Terminology as Topic
- topic2: Phylogeny
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 16049 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22062) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-22062 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 32730 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/17514 | ||||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 78006 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID46956.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
| 120548 | Curators of the CIP | Collection of Institut Pasteur (CIP 105489) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105489 |