Strain identifier

BacDive ID: 8613

Type strain: Yes

Species: Metamycoplasma orale

Strain Designation: CH19299, CH 19299

Strain history: CIP <- 1996, NCTC <- 1967, MRL, PHLS, Colindale, London, UK <- R.M. Chanock, NIH, MD, USA: strain CH 19299

NCBI tax ID(s): 2121 (species)

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General

@ref: 18055

BacDive-ID: 8613

DSM-Number: 25590

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative, human pathogen

description: Metamycoplasma orale CH19299 is a mesophilic, Gram-negative human pathogen that was isolated from Oropharynx of child.

NCBI tax id

  • NCBI tax id: 2121
  • Matching level: species

strain history

@refhistory
18055<- NBRC <- K. Yamamoto; CH19299
121427CIP <- 1996, NCTC <- 1967, MRL, PHLS, Colindale, London, UK <- R.M. Chanock, NIH, MD, USA: strain CH 19299

doi: 10.13145/bacdive8613.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/mycoplasmatota
  • domain: Bacteria
  • phylum: Mycoplasmatota
  • class: Mollicutes
  • order: Mycoplasmoidales
  • family: Metamycoplasmataceae
  • genus: Metamycoplasma
  • species: Metamycoplasma orale
  • full scientific name: Metamycoplasma orale (Taylor-Robinson et al. 1964) Gupta et al. 2018

  • synonyms

    • @ref: 20215
    • synonym: Mycoplasma orale

@ref: 18055

domain: Bacteria

phylum: Tenericutes

class: Mollicutes

order: Mycoplasmatales

family: Metamycoplasmataceae

genus: Metamycoplasma

species: Metamycoplasma orale

full scientific name: Metamycoplasma orale (Taylor-Robinson et al. 1964) Gupta et al. 2018

strain designation: CH19299, CH 19299

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.808
121427negativerod-shapedno

colony morphology

  • @ref: 18055
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
18055SP4-Z MEDIUM (DSMZ Medium 1076b)yeshttps://mediadive.dsmz.de/medium/1076bName: SP4-Z MEDIUM (DSMZ Medium 1076b) Composition: Agar 10.0 g/l Tryptone 10.0 g/l Bacto peptone 5.0 g/l PPLO broth 3.5 g/l Urea 2.0 g/l Glucose 1.0 g/l None 1.0 g/l Yeast extract 0.4 g/l DNA 0.2 g/l CMRL 1066 Fetal bovine serum Swine serum Distilled water
121427CIP Medium 749yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=749
121427CIP Medium 89yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=89

culture temp

  • @ref: 18055
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no100
69480no99.998

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
18055Oropharynx of childWashington, DCUSAUSANorth America
121427Human, Child, oropharynxWashington, WashingtonUnited States of AmericaUSANorth America

isolation source categories

Cat1Cat2Cat3
#Infection#Patient
#Host Body-Site#Oral cavity and airways
#Host#Human#Child

taxonmaps

  • @ref: 69479
  • File name: preview.99_6249.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15862;96_1634;97_3659;98_4629;99_6249&stattab=map
  • Last taxonomy: Mycoplasma
  • 16S sequence: AY796060
  • Sequence Identity:
  • Total samples: 7584
  • soil counts: 93
  • aquatic counts: 118
  • animal counts: 7303
  • plant counts: 70

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
18055yes, in single cases1Risk group (German classification)
1214272Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mycoplasma orale gene for 16S rRNA, partial sequenceAB069816245ena2121
20218Mycoplasma orale 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAY737010882ena2121
18055Mycoplasma orale strain NC10112 16S ribosomal RNA gene, complete sequenceAY7960601510ena2121

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Metamycoplasma orale ATCC 23714GCA_000420105contigncbi1266996
66792Metamycoplasma orale NCTC10112GCA_900660435completencbi2121
66792Mycoplasma orale ATCC 237141266996.3wgspatric1266996
66792Mycoplasma orale strain NCTC101122121.6completepatric2121
66792Mycoplasma orale ATCC 237142523533589draftimg1266996

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno96.989no
gram-positiveyes53.285no
anaerobicno93.473no
aerobicno94.657no
halophileno54.372no
spore-formingno98.139no
thermophileno98.835yes
glucose-utilyes83.832no
motileno93.36no
glucose-fermentyes71.7no

External links

@ref: 18055

culture collection no.: DSM 25590, ATCC 23714, CIP 104969, IFO (now NBRC) 14477, NCTC 10112

straininfo link

  • @ref: 77996
  • straininfo: 38198

literature

  • topic: Cultivation
  • Pubmed-ID: 14329470
  • title: SUPPRESSION OF ROUS SARCOMA VIRUS GROWTH IN TISSUE CULTURES BY MYCOPLASMA ORALE.
  • authors: SOMERSON NL, COOK MK
  • journal: J Bacteriol
  • DOI: 10.1128/jb.90.2.534-540.1965
  • year: 1965
  • mesh: *Agar, Animals, *Antigen-Antibody Reactions, *Avian Sarcoma Viruses, Chick Embryo, *Complement Fixation Tests, *Culture Media, *Fibroblasts, *Mycoplasma, *Mycoplasma orale, *Research, *Rous sarcoma virus, *Tissue Culture Techniques, *Virus Cultivation

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
18055Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25590)https://www.dsmz.de/collection/catalogue/details/culture/DSM-25590
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77996Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID38198.1StrainInfo: A central database for resolving microbial strain identifiers
121427Curators of the CIPCollection of Institut Pasteur (CIP 104969)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104969