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Strain identifier

BacDive ID: 8604

Type strain: Yes

Species: Mycoplasmoides gallisepticum

Strain Designation: X95, PG 31

Strain history: <- J. Davis <- D. G. ff. Edward; PG 31 <- F. Markham

NCBI tax ID(s): 2096 (species)

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General

@ref: 8334

BacDive-ID: 8604

DSM-Number: 19817

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, animal pathogen

description: Mycoplasmoides gallisepticum X95 is a mesophilic animal pathogen that was isolated from chickens, respiratory tract.

NCBI tax id

  • NCBI tax id: 2096
  • Matching level: species

strain history: <- J. Davis <- D. G. ff. Edward; PG 31 <- F. Markham

doi: 10.13145/bacdive8604.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/mycoplasmatota
  • domain: Bacteria
  • phylum: Mycoplasmatota
  • class: Mollicutes
  • order: Mycoplasmoidales
  • family: Mycoplasmoidaceae
  • genus: Mycoplasmoides
  • species: Mycoplasmoides gallisepticum
  • full scientific name: Mycoplasmoides gallisepticum (Edward and Kanarek 1960) Gupta et al. 2018

  • synonyms

    • @ref: 20215
    • synonym: Mycoplasma gallisepticum

@ref: 8334

domain: Bacteria

phylum: Tenericutes

class: Mollicutes

order: Mycoplasmatales

family: Mycoplasmoidaceae

genus: Mycoplasmoides

species: Mycoplasmoides gallisepticum

full scientific name: Mycoplasmoides gallisepticum (Edward and Kanarek 1960) Gupta et al. 2018

strain designation: X95, PG 31

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: negative
  • confidence: 70.221

colony morphology

  • @ref: 8334
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8334SP4-Z MEDIUM (DSMZ Medium 1076b)yeshttps://mediadive.dsmz.de/medium/1076bName: SP4-Z MEDIUM (DSMZ Medium 1076b) Composition: Agar 10.0 g/l Tryptone 10.0 g/l Bacto peptone 5.0 g/l PPLO broth 3.5 g/l Urea 2.0 g/l Glucose 1.0 g/l None 1.0 g/l Yeast extract 0.4 g/l DNA 0.2 g/l CMRL 1066 Fetal bovine serum Swine serum Distilled water
41399MEDIUM 88 - for Mycoplasma and AcholeplasmayesDistilled water make up to (670.000 ml);PPLO broth (17.000 g);Sterile supplement - M00273 (210.000 ml);Yeast extract solution 25 % - M00263 (100.000 ml);0.1 % Phenol red - M00387 (20.000 ml)

culture temp

@refgrowthtypetemperaturerange
8334positivegrowth37mesophilic
41399positivegrowth37mesophilic

Physiology and metabolism

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 98.855

halophily

  • @ref: 69480
  • halophily level: non-halophilic
  • confidence: 93.372

Isolation, sampling and environmental information

isolation

  • @ref: 8334
  • sample type: chickens, respiratory tract
  • country: United Kingdom
  • origin.country: GBR
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Host#Birds#Chicken
#Host Body-Site#Oral cavity and airways#Airways

taxonmaps

  • @ref: 69479
  • File name: preview.99_629.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_63;96_395;97_446;98_510;99_629&stattab=map
  • Last taxonomy: Mycoplasma gallisepticum
  • 16S sequence: JX843736
  • Sequence Identity:
  • Total samples: 606
  • soil counts: 7
  • aquatic counts: 109
  • animal counts: 490

Safety information

risk assessment

  • @ref: 8334
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mycoplasma gallisepticum 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAY7449421288ena2096
20218Mycoplasma gallisepticum strain ATCC 19610 16S ribosomal RNA gene, partial sequenceJX8437361458ena2096
8334Mycoplasma gallisepticum gene for 16S rRNA, partial sequence, strain: NBRC 14855AB6806861442ena2096

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycoplasma gallisepticum NCTC10115GCA_900476085completencbi2096
66792Mycoplasma gallisepticum ATCC 19610 ATCC 19610GCA_000428645scaffoldncbi1267002
66792Mycoplasma gallisepticum ATCC 196101267002.3wgspatric1267002
66792Mycoplasma gallisepticum strain NCTC101152096.161completepatric2096
66792Mycoplasma gallisepticum NCTC 101152847356762completeimg2096
66792Mycoplasma gallisepticum ATCC 196102523533588draftimg1267002
66792uncultured Ruminococcus sp. RUG11235GCA_902767185contigpatric165186
66792Bacteroidetes bacterium JR-MC-H-240519-122GCA_947380625contigpatric1898104

External links

@ref: 8334

culture collection no.: DSM 19817, ATCC 19610, CIP 104967, IFO 14855, NCTC 10115

straininfo link

@refpassport
20218http://www.straininfo.net/strains/764088
20218http://www.straininfo.net/strains/100250
20218http://www.straininfo.net/strains/153126
20218http://www.straininfo.net/strains/222165
20218http://www.straininfo.net/strains/100251

literature

topicPubmed-IDtitleauthorsjournalyearmeshDOItopic2
Enzymology2649056Monoclonal antibodies that recognize specific antigens of Mycoplasma gallisepticum and M. synoviae.Hwang YS, Panangala VS, Rossi CR, Giambrone JJ, Lauerman LHAvian Dis1989Animals, Antibodies, Monoclonal/*immunology, Antigens, Bacterial/*analysis, Autoradiography, Blotting, Western, Electrophoresis, Polyacrylamide Gel, Fluorescent Antibody Technique, Mycoplasma/*immunology, Peptides/analysis, Vero Cells
Metabolism8021195Physical map of Mycoplasma gallisepticum.Tigges E, Minion FCJ Bacteriol1994Base Sequence, DNA Transposable Elements, DNA, Bacterial/metabolism, Deoxyribonucleases, Type II Site-Specific/metabolism, Electrophoresis, Agar Gel/methods, Genetic Linkage, Molecular Sequence Data, Mycoplasma/*genetics, *Restriction Mapping10.1128/jb.176.13.4157-4159.1994Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8334Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19817)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19817
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41399Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16934
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)