Strain identifier

BacDive ID: 8547

Type strain: Yes

Species: Mycobacterium abscessus subsp. bolletii

Strain Designation: BD

Strain history: CIP <- 2004, M. Drancourt, Timone Hosp., Marseille, France: strain BD

NCBI tax ID(s): 1091046 (strain), 319705 (subspecies)

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General

@ref: 15460

BacDive-ID: 8547

DSM-Number: 45149

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, human pathogen

description: Mycobacterium abscessus subsp. bolletii BD is an aerobe, mesophilic human pathogen that was isolated from sputum.

NCBI tax id

NCBI tax idMatching level
319705subspecies
1091046strain

strain history

@refhistory
422112004, M. Drancourt, Hosp. La Timone, Marseille, France: strain BD
15460<- CIP; CIP 108541 <- M. Drancourt, Hosp. La Timone, Marseille, France; BD
67770CCUG 50184 <-- M. Drancourt strain BD.
115981CIP <- 2004, M. Drancourt, Timone Hosp., Marseille, France: strain BD

doi: 10.13145/bacdive8547.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium abscessus subsp. bolletii
  • full scientific name: Mycobacterium abscessus subsp. bolletii (Adékambi et al. 2006) Leao et al. 2011

  • synonyms

    @refsynonym
    20215Mycobacterium bolletii
    20215Mycobacteroides abscessus subsp. bolletii

@ref: 15460

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium abscessus subsp. bolletii

full scientific name: Mycobacterium abscessus subsp. bolletii (Adékambi et al. 2006) Leao et al. 2011 emend. Tortoli et al. 2016 emend. Nouioui et al. 2018

strain designation: BD

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no96.361
69480100positive
115981nopositiverod-shaped

colony morphology

  • @ref: 58774
  • incubation period: 2-3 days

multimedia

  • @ref: 15460
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_45149.jpg
  • caption: Medium 645 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
42211MEDIUM 93 - for Mycobacterium avium subsp. sylvaticumyesDistilled water make up to (900.000 ml);Glycerol (4.500 ml);Middlebrook7H10 agar (17.000 g);Middelbrook OADC enrichment (100.000 ml)
15460MIDDLEBROOK MEDIUM (DSMZ Medium 645)yesName: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled waterhttps://mediadive.dsmz.de/medium/645
115981CIP Medium 93yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=93

culture temp

@refgrowthtypetemperaturerange
42211positivegrowth30mesophilic
58774positivegrowth37mesophilic
15460positivegrowth30mesophilic
67770positivegrowth37mesophilic
115981positivegrowth22-37
115981nogrowth10psychrophilic
115981nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
58774aerobe
115981obligate aerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.769

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
115981606565hippurate-hydrolysis
11598117632nitrate-reduction
11598116301nitrite-reduction
11598117632nitrate-respiration

antibiotic resistance

  • @ref: 115981
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 115981
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
115981oxidase-
115981beta-galactosidase-3.2.1.23
115981gelatinase-
115981amylase-
115981DNase-
115981caseinase-3.4.21.50
115981catalase+1.11.1.6
115981tween esterase-
115981gamma-glutamyltransferase+2.3.2.2
115981lecithinase-
115981lipase-
115981protease-
115981urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
115981--++-+-+-------+----

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
115981+--++-------------+------------+--+------------------+++---++-----------+----+-++---+---+-+--++-+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeisolation date
15460sputumMarseilleFranceFRAEurope43.29655.3698
58774Human bronchial lavageMarseilleFranceFRAEurope
67770Sputum
115981Broncho-alveolar liquidMarseilleFranceFRAEurope2001

isolation source categories

  • Cat1: #Host Body-Site
  • Cat2: #Oral cavity and airways

taxonmaps

  • @ref: 69479
  • File name: preview.99_54.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_44;97_46;98_50;99_54&stattab=map
  • Last taxonomy: Mycobacteroides
  • 16S sequence: AY859681
  • Sequence Identity:
  • Total samples: 21994
  • soil counts: 7046
  • aquatic counts: 6199
  • animal counts: 7007
  • plant counts: 1742

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
15460yesyes2Risk group (German classification)
1159812Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mycobacterium bolletii gene for 16S ribosomal RNA, partial sequenceAB5486061468ena319705
20218Mycobacterium bolletii DNA, 16S-23S ribosomal RNA intergenic spacer, partial sequence, strain: JCM 15297AB548607297ena319705
20218Mycobacterium bolletii strain CCUG 50184 16S ribosomal RNA gene, partial sequenceDQ987740529ena319705
20218Mycobacterium bolletii strain CCUG 50184 16S-23S ribosomal RNA intergenic spacer, partial sequence; and 23S ribosomal RNA gene, complete sequenceGU1438883401ena319705
15460Mycobacteroides abscessus subsp. bolletii strain CIP 108541 16S ribosomal RNA gene, partial sequenceAY8596811481ena319705

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycobacterium abscessus subsp. bolletii BD1091046.3wgspatric1091046
66792Mycobacterium abscessus subsp. bolletii strain BD319705.54wgspatric319705
66792Mycobacterium abscessus subsp. bolletii strain CIP 108541319705.16wgspatric319705
66792Mycobacterium abscessus subsp. bolletii strain CIP108541319705.59wgspatric319705
66792Mycobacteroides abscessus subsp. bolletii BD1091046.5completepatric1091046
66792Mycobacteroides abscessus subsp. bolletii strain CCUG 50184319705.134wgspatric319705
67770Mycobacteroides abscessus subsp. bolletii BDGCA_000239035contigncbi1091046
67770Mycobacteroides abscessus subsp. bolletii CIP 108541GCA_001792615contigncbi319705
67770Mycobacteroides abscessus subsp. bolletii CCUG 50184GCA_006409125contigncbi319705
67770Mycobacteroides abscessus subsp. bolletii BDGCA_002013835contigncbi319705
67770Mycobacteroides abscessus subsp. bolletii CIP108541GCA_002086525contigncbi319705

GC content

@refGC-contentmethod
1546064.07sequence analysis
6777064genome sequence analysis
6777061-65high performance liquid chromatography (HPLC)
6777064.1genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno82no
motileno95.692no
flagellatedno98.332no
gram-positiveyes89.986no
anaerobicno99.586yes
aerobicyes89.505yes
halophileno93.896no
spore-formingno79.236no
glucose-utilyes81.213no
thermophileno99.446no
glucose-fermentno93.798no

External links

@ref: 15460

culture collection no.: DSM 45149, CCUG 50184, CIP 108541, JCM 15297, BCRC 16915

straininfo link

  • @ref: 77931
  • straininfo: 289776

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16403878rpoB gene sequence-based characterization of emerging non-tuberculous mycobacteria with descriptions of Mycobacterium bolletii sp. nov., Mycobacterium phocaicum sp. nov. and Mycobacterium aubagnense sp. nov.Adekambi T, Berger P, Raoult D, Drancourt MInt J Syst Evol Microbiol10.1099/ijs.0.63969-02006Anti-Bacterial Agents/pharmacology, Bacterial Proteins/*genetics, Chaperonin 60, Chaperonins/genetics, DNA-Directed RNA Polymerases/*genetics, *Genes, Bacterial, Humans, Microbial Sensitivity Tests, Molecular Sequence Data, Mycobacterium/chemistry/*classification/drug effects/physiology, Mycobacterium Infections/diagnosis/*microbiology, Species Specificity, Superoxide Dismutase/geneticsPathogenicity
Transcriptome21106786Multilocus sequence analysis and rpoB sequencing of Mycobacterium abscessus (sensu lato) strains.Macheras E, Roux AL, Bastian S, Leao SC, Palaci M, Sivadon-Tardy V, Gutierrez C, Richter E, Rusch-Gerdes S, Pfyffer G, Bodmer T, Cambau E, Gaillard JL, Heym BJ Clin Microbiol10.1128/JCM.01274-102010Bacterial Proteins/genetics, *Bacterial Typing Techniques, Brazil, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA-Directed RNA Polymerases/*genetics, France, Germany, Humans, Molecular Sequence Data, *Multilocus Sequence Typing, Mycobacterium/*classification/*genetics, Phylogeny, Sequence Analysis, DNA, SwitzerlandPhylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
15460Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45149)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45149
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
42211Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6175
58774Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 50184)https://www.ccug.se/strain?id=50184
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
77931Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID289776.1StrainInfo: A central database for resolving microbial strain identifiers
115981Curators of the CIPCollection of Institut Pasteur (CIP 108541)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108541