Archive BacDiveID:10.13145/bacdive8498.20230509.8.1

Strain identifier

BacDive ID: 8498

Type strain:

Species: Mycobacterium botniense

Strain Designation: E 347

Strain history: CCUG 47976 <-- P. Torkko <-- E. Tortoli E347.

NCBI tax ID(s): 84962 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 11843

BacDive-ID: 8498

DSM-Number: 44537

keywords: genome sequence, 16S sequence, Bacteria, aerobe, thermophilic

description: Mycobacterium botniense E 347 is an aerobe, thermophilic bacterium that was isolated from brook water.

NCBI tax id

NCBI tax id: 84962
Matching level: species

strain history

@ref	history
11843	<- E. Tortoli, Coreggi Hospital, Florence; E 347
67770	CCUG 47976 <-- P. Torkko <-- E. Tortoli E347.

doi: 10.13145/bacdive8498.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/actinomycetota
domain: Bacteria
phylum: Actinomycetota
class: Actinomycetes
order: Mycobacteriales
family: Mycobacteriaceae
genus: Mycobacterium
species: Mycobacterium botniense
full scientific name: Mycobacterium botniense Torkko et al. 2000

@ref: 11843

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium botniense

full scientific name: Mycobacterium botniense Torkko et al. 2000

strain designation: E 347

type strain: yes

Morphology

cell morphology

@ref	motility	confidence	gram stain
69480	no	96.382
69480		100	positive

colony morphology

@ref: 57938
incubation period: >21 days

multimedia

@ref: 11843
multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44537.jpg
caption: Medium 645 45°C
intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@ref	name	growth	link	composition
11843	MIDDLEBROOK MEDIUM (DSMZ Medium 645)	yes	https://mediadive.dsmz.de/medium/645	Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
11843	MIDDLEBROCK 7H11 AGAR (DSMZ Medium 911)	yes	https://mediadive.dsmz.de/medium/911	Name: MIDDLEBROCK 7H11 AGAR (DSMZ Medium 911) Composition: Bovine albumin 5.0 g/l Dextrose 2.0 g/l NaCl 0.85 g/l Agar 0.350001 g/l Oleic acid 0.05 g/l KH2PO4 0.0350001 g/l Na2HPO4 0.0350001 g/l Pancreatic digest of casein 0.0233333 g/l L-Glutamic acid 0.0116667 g/l (NH4)2SO4 0.0116667 g/l Sodium citrate 0.00933332 g/l Catalase 0.004 g/l MgSO4 0.00116667 g/l Ferric ammonium citrate 0.000933332 g/l Pyridoxine 2.33333e-05 g/l Malachite green 2.33333e-05 g/l Biotin 1.16667e-05 g/l Glycerol Distilled water

culture temp

@ref	growth	type	temperature	range
11843	positive	growth	45	thermophilic
57938	positive	growth	45	thermophilic
67770	positive	growth	45	thermophilic

Physiology and metabolism

oxygen tolerance

@ref: 57938
oxygen tolerance: aerobe

spore formation

@ref: 69480
spore formation: no
confidence: 99.777

Isolation, sampling and environmental information

isolation

@ref	sample type	geographic location	country	origin.country	continent
11843	brook water	Botnia	Finland	FIN	Europe
57938	Brook water	Botnia	Finland	FIN	Europe
67770	Stream water		Finland	FIN	Europe

isolation source categories

Cat1: #Environmental
Cat2: #Aquatic
Cat3: #River (Creek)

Safety information

risk assessment

@ref: 11843
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref	description	accession	length	database	NCBI tax ID
20218	Mycobacterium botniense strain DSM 44537 16S ribosomal RNA gene, partial sequence	AF547902	558	ena	84962
20218	Mycobacterium botniense 16S rRNA gene and internal transcribed spacer 1	AJ012756	1787	ena	84962

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Mycobacterium botniense JCM 17322	GCA_010723305	contig	ncbi	84962
66792	Mycobacterium botniense strain JCM 17322	84962.6	wgs	patric	84962

Genome-based predictions

predictions

trait	prediction	confidence	training_data
spore-forming	no	73	no
motile	no	97.081	no
flagellated	no	99.202	no
gram-positive	yes	89.143	no
anaerobic	no	98.318	yes
halophile	no	88.771	no
spore-forming	no	87.829	no
glucose-util	yes	67.464	no
aerobic	yes	87.629	yes
thermophile	yes	59.832	yes
glucose-ferment	no	91.306	no

External links

@ref: 11843

culture collection no.: DSM 44537, ATCC 700701, CCUG 47450, CCUG 47976, JCM 17322, CIP 106753, NCIMB 13713

straininfo link

@ref: 77891
straininfo: 44091

Reference

@id	authors	catalogue	doi/url	title	journal	pubmed
11843	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44537)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-44537
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.		10.1016/j.syapm.2013.11.002	StrainInfo introduces electronic passports for microorganisms.	Syst Appl Microbiol. 37: 42-50 2014	24321274
57938	Curators of the CCUG	Culture Collection University of Gothenburg (CCUG) (CCUG 47976)	https://www.ccug.se/strain?id=47976
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770	Curators of the JCM		https://jcm.brc.riken.jp/en/
69480	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
77891	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID44091.1	StrainInfo: A central database for resolving microbial strain identifiers