Strain identifier

BacDive ID: 8437

Type strain: Yes

Species: Mycobacterium gadium

Strain Designation: 141280001

Strain history: CIP <- 1998, V. Vincent, Inst. Pasteur, Paris, France: strain 14 128 0001 <- ATCC <- M. Casal

NCBI tax ID(s): 1794 (species)

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General

@ref: 11476

BacDive-ID: 8437

DSM-Number: 44077

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, human pathogen

description: Mycobacterium gadium 141280001 is a microaerophile, mesophilic, Gram-positive human pathogen that was isolated from sputum.

NCBI tax id

  • NCBI tax id: 1794
  • Matching level: species

strain history

@refhistory
11476<- S. Rüsch-Gerdes, Borstel <- ATCC <- M. Casal
67770CIP 105388 <-- V. Vincent <-- ATCC 27726 <-- M. Casal.
119502CIP <- 1998, V. Vincent, Inst. Pasteur, Paris, France: strain 14 128 0001 <- ATCC <- M. Casal

doi: 10.13145/bacdive8437.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium gadium
  • full scientific name: Mycobacterium gadium Casal and Calero 1974 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Mycolicibacterium gadium

@ref: 11476

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium gadium

full scientific name: Mycobacterium gadium Casal and Calero 1974

strain designation: 141280001

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
119502rod-shapedno

colony morphology

  • @ref: 53709
  • incubation period: >3 days

multimedia

  • @ref: 11476
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44077.jpg
  • caption: Medium 645 37°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11476MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
11476LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354)yeshttps://mediadive.dsmz.de/medium/354Name: LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) Composition: Potato flour 18.6104 g/l L-Asparagin 2.23325 g/l KH2PO4 1.55087 g/l Mg-citrate 0.372208 g/l Malachite green 0.248139 g/l MgSO4 0.148883 g/l Glycerol Fresh egg mixture Distilled water
36384MEDIUM 55 - for Mycobacteriumyes
119502CIP Medium 55yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55

culture temp

@refgrowthtypetemperaturerange
11476positivegrowth37mesophilic
36384positivegrowth37mesophilic
53709positivegrowth37mesophilic
67770positivegrowth37mesophilic
119502positivegrowth22-37
119502nogrowth10psychrophilic
119502nogrowth41thermophilic
119502nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
53709microaerophile
119502obligate aerobe

spore formation

@refspore formationconfidence
69481no91
69480no99.909

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
119502606565hippurate-hydrolysis
11950217632nitrate+reduction
11950216301nitrite-reduction
11950217632nitrate-respiration

metabolite production

  • @ref: 119502
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
119502oxidase-
119502beta-galactosidase-3.2.1.23
119502gelatinase-
119502amylase+
119502DNase-
119502caseinase-3.4.21.50
119502catalase+1.11.1.6
119502tween esterase-
119502gamma-glutamyltransferase-2.3.2.2
119502lecithinase-
119502lipase-
119502protease-
119502urease+3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119502--++-+++--++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119502++------------------------+-----+----------------+----------+----+------++-+--+++-------+-------+--

Isolation, sampling and environmental information

isolation

@refsample type
11476sputum
53709Human sputum
67770Sputum of a patient with pulmonary disease
119502Sputum

isolation source categories

  • Cat1: #Host Body Product
  • Cat2: #Fluids
  • Cat3: #Sputum

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
11476yes, in single cases1Risk group (German classification)
1195021Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218M.gadium 16S ribosomal rRNAM616631352ena1794
20218M.gadium 16S ribosomal RNA, partX555941456ena1794
20218Mycobacterium gadium strain CIP 105388 16S ribosomal RNA gene, partial sequenceAF547926541ena1794
20218Mycobacterium gadium strain CIP 105388 16S ribosomal RNA gene, partial sequenceDQ4733101487ena1794

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycolicibacterium gadium JCM 12688GCA_010728925completencbi1794
66792Mycolicibacterium gadium strain JCM 126881794.9completepatric1794
66792Mycolicibacterium gadium DSM 44077GCA_025821365scaffoldncbi1794

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno91no
motileno90.985no
flagellatedno98.106no
gram-positiveyes89.343no
anaerobicno99.283yes
aerobicyes90.478no
halophileno89.704no
spore-formingno84.655no
thermophileno99.399yes
glucose-utilyes87.224no
glucose-fermentno91.798no

External links

@ref: 11476

culture collection no.: DSM 44077, ATCC 27726, CCUG 37515, CIP 105388, NCTC 10942, HAMBI 2274, JCM 12688

straininfo link

  • @ref: 77836
  • straininfo: 39144

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11476Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44077)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44077
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36384Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17402
53709Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 37515)https://www.ccug.se/strain?id=37515
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77836Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID39144.1StrainInfo: A central database for resolving microbial strain identifiers
119502Curators of the CIPCollection of Institut Pasteur (CIP 105388)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105388