Mycobacterium gadium (DSM 44077, ATCC 27726, CCUG 37515)

Name: Mycobacterium gadium (DSM 44077, ATCC 27726, CCUG 37515)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 8437

Type strain

Strain Designation 141280001

Culture col. no. DSM 44077 ATCC 27726 CCUG 37515 CIP 105388 NCTC 10942 3 more

NCBI tax ID(s) 1794

Special Collections DSMZ CCUG JCM CIP

History DSMZ <- S. Rüsch-Gerdes, Borstel <- ATCC <- M. Casal JCM <-- CIP 105388 <-- V. Vincent <-- ATCC 27726 <-- M. Casal. CIP <- 1998, V. Vincent, Inst. Pasteur, Paris, France: strain 14 128 0001 <- ATCC <- M. Casal

Links

Mycobacterium gadium 141280001 is a microaerophile, rod-shaped bacterium that was isolated from sputum.

rod-shaped microaerophile genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 39144

CT1978-06995,CT2025-00071,ATCC 27726,CCUG 37515,DSM 44077,CIP 105388,NCTC 10942,JCM 12688,HAMBI 2274,CNCTC 7272

@ref 20215

Last LPSN update 2026-05-29 08:26:52

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Mycobacteriales

Family Mycobacteriaceae

Genus Mycobacterium

Species Mycobacterium gadium

Full scientific name Mycobacterium gadium Casal and Calero 1974 (Approved Lists 1980)

Synonyms (1)

Mycolicibacterium gadium

Morphology

Cell morphology

@ref	Cell shape	Gram stain	Confidence
119502	rod-shaped
125439		positive	90.977
125438			90.5

Colony morphology

53709

Incubation period>3 days

Multimedia

@ref:	11476
multimedia content:	DSM_44077.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_44077.jpg
caption:	Medium 645 37°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
11476	MIDDLEBROOK MEDIUM (DSMZ Medium 645)	Medium recipe at MediaDive	Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
11476	LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354)	Medium recipe at MediaDive	Name: LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) Composition: Potato flour 18.6104 g/l L-Asparagin 2.23325 g/l KH2PO4 1.55087 g/l Mg-citrate 0.372208 g/l Malachite green 0.248139 g/l MgSO4 0.148883 g/l Glycerol Fresh egg mixture Distilled water
36384	MEDIUM 55 - for Mycobacterium
119502	CIP Medium 55	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
11476	positive	growth	37
36384	positive	growth	37
53709	positive	growth	37
67770	positive	growth	37
119502	positive	growth	22-37
119502	negative	growth	10
119502	negative	growth	41
119502	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
53709	microaerophile
119502	obligate aerobe
125439	obligate aerobe	99.208

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
119502	606565 ChEBI	hippurate	-	hydrolysis
119502	17632 ChEBI	nitrate	+	reduction
119502	17632 ChEBI	nitrate	-	respiration
119502	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
119502	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
119502	amylase	+
119502	beta-galactosidase	-	3.2.1.23
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
119502	caseinase	-	3.4.21.50
119502	catalase	+	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
119502	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
119502	gamma-glutamyltransferase	-	2.3.2.2
119502	gelatinase	-
119502	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
119502	lipase	-
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
119502	oxidase	-
119502	protease	-
68382	trypsin	-	3.4.21.4	from API zym
119502	tween esterase	-
119502	urease	+	3.5.1.5
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
119502	-	-	+	+	-	+	+	+	-	-	+	+	-	-	-	-	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
119502	not determinedn.d.	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	+	-	-	-	-	-	-	+	+	-	+	-	-	+	+	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host Body Product	#Fluids	#Sputum

Isolation

@ref	Sample type	Host species
11476	sputum
53709	Human sputum	Homo sapiens
67770	Sputum of a patient with pulmonary disease	Homo sapiens
119502	Sputum

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Biosafety level	Biosafety level comment
11476	yes, in single cases	1	Risk group (German classification) According to TRBA 466
119502		1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1072892v1 assembly for Mycolicibacterium gadium JCM 12688	complete	GCA_010728925	1794.9	8110680197	1794	96.95
66792	ASM2582136v1 assembly for Mycolicibacterium gadium DSM 44077	scaffold	GCA_025821365	1794.11		1794	0

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Mycolicibacterium gadium ribosomal RNA, complete sequence	M61663	1352	1794
20218	M.gadium 16S ribosomal RNA, part	X55594	1456	1794
20218	Mycobacterium gadium strain CIP 105388 16S ribosomal RNA gene, partial sequence	AF547926	541	1794
20218	Mycobacterium gadium strain CIP 105388 16S ribosomal RNA gene, partial sequence	DQ473310	1487	1794
124043	Mycolicibacterium gadium strain CIP 105388 16S ribosomal RNA gene, partial sequence.	MN005945	1451	1794

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM1072892v1 assembly for Mycolicibacterium gadium JCM 12688
BV-BRC: 1794.9

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.21	no
125439	gram_stain	BacteriaNetⓘ	positive	90.98	no
125439	motility	BacteriaNetⓘ	no	87.58	no
125439	spore_formation	BacteriaNetⓘ	no	71.58	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Mycolicibacterium gadium JCM 12688
NCBI: GCA_010728925.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	89.02	no
125438	anaerobic	anaerobicⓘ	no	98.14	yes
125438	aerobic	aerobicⓘ	yes	84.63	no
125438	spore-forming	spore-formingⓘ	no	59.99	no
125438	thermophilic	thermophileⓘ	no	95.50	yes
125438	flagellated	motile2+ⓘ	no	90.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Phylogenomics and Comparative Genomic Studies Robustly Support Division of the Genus Mycobacterium into an Emended Genus Mycobacterium and Four Novel Genera.	Gupta RS, Lo B, Son J.	Front Microbiol	10.3389/fmicb.2018.00067	2018
	In Vitro Activity of the Sudapyridine (WX-081) against Non-Tuberculous Mycobacteria Isolated in Beijing, China.	Zhu R, Shang Y, Chen S, Xiao H, Ren R, Wang F, Xue Y, Li L, Li Y, Chu N, Huang H.	Microbiol Spectr	10.1128/spectrum.01372-22	2022
	In vitro and intracellular inhibitory activities of nosiheptide against Mycobacterium abscessus.	Zhu R, Yu X, Zhang T, Kong Y, Wang F, Jia J, Xue Y, Huang H.	Front Microbiol	10.3389/fmicb.2022.926361	2022
Pathogenicity	In Vitro Activities of Bedaquiline and Delamanid against Nontuberculous Mycobacteria Isolated in Beijing, China.	Yu X, Gao X, Li C, Luo J, Wen S, Zhang T, Ma Y, Dong L, Wang F, Huang H.	Antimicrob Agents Chemother	10.1128/aac.00031-19	2019
Phylogeny	Biochip system for rapid and accurate identification of mycobacterial species from isolates and sputum.	Zhu L, Jiang G, Wang S, Wang C, Li Q, Yu H, Zhou Y, Zhao B, Huang H, Xing W, Mitchelson K, Cheng J, Zhao Y, Guo Y.	J Clin Microbiol	10.1128/jcm.00158-10	2010
	Compilation of small ribosomal subunit RNA structures.	Neefs JM, Van de Peer Y, De Rijk P, Chapelle S, De Wachter R.	Nucleic Acids Res	10.1093/nar/21.13.3025	1993
Metabolism	Intracellular localization of the mycobacterial stressosome complex.	Ramesh M, Nitharwal RG, Behra PRK, Fredrik Pettersson BM, Dasgupta S, Kirsebom LA.	Sci Rep	10.1038/s41598-021-89069-8	2021
Pathogenicity	Rapid Detection of Mycobacterium tuberculosis Strains Resistant to Isoniazid and/or Rifampicin: Standardization of Multiplex Polymerase Chain Reaction Analysis.	Collantes J, Solari FB, Rigouts L.	Am J Trop Med Hyg	10.4269/ajtmh.16-0120	2016
Metabolism	Lipid domains of mycobacteria studied with fluorescent molecular probes.	Christensen H, Garton NJ, Horobin RW, Minnikin DE, Barer MR.	Mol Microbiol	10.1046/j.1365-2958.1999.01304.x	1999
Pathogenicity	Preliminary study of the in vitro activity of irloxacin against mycobacteria.	Casal M, Gutierrez J, Ruiz P, Moreno G.	Chemotherapy	10.1159/000239345	1995
Phylogeny	Separation of Mycobacterium gadium from other rapidly growing mycobacteria on the basis of DNA homology and restriction endonuclease analysis.	Garcia MJ, Tabares E.	J Gen Microbiol	10.1099/00221287-132-8-2265	1986
	Mycolic acid patterns of some rapidly-growing species of Mycobacterium.	Minnikin DE, Minnikin SM, Parlett JH, Goodfellow M.	Zentralbl Bakteriol Mikrobiol Hyg A	10.1016/s0176-6724(85)80076-6	1985
Genetics	Characterization of Mycobacterium salfingeri sp. nov.: A novel nontuberculous mycobacteria isolated from a human wound infection.	Musser E, Smith C, Halse TA, Kohlerschmidt D, Rourke A, Fiero A, Musser KA, Escuyer V, Lapierre P.	Front Microbiol	10.3389/fmicb.2022.992610	2022
Phylogeny	Mycobacterium barrassiae sp. nov., a Mycobacterium moriokaense group species associated with chronic pneumonia.	Adekambi T, Raoult D, Drancourt M.	J Clin Microbiol	10.1128/jcm.00724-06	2006

References

#11476	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44077
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#36384	; Curators of the CIP;
#53709	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 37515
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#119502	Collection of Institut Pasteur ; Curators of the CIP; CIP 105388
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Mycobacterium gadium (DSM 44077, ATCC 27726, CCUG 37515)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Metabolite utilization

Metabolite production

Enzymes

API zym

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_010728925

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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