Strain identifier
BacDive ID: 8349
Type strain: 
Species: Mycobacterium engbaekii
Strain Designation: Copenhagen 20538, Eng 1
Strain history: <- ATCC; ATCC 27353 <- T. Korsak <- H. Boisvert <- Copenhagen, Denmark 20538
NCBI tax ID(s): 188915 (species)
version 9.1 (current version)
General
@ref: 18059
BacDive-ID: 8349
DSM-Number: 45694
keywords: genome sequence, 16S sequence, Bacteria, coccus-shaped, human pathogen
description: Mycobacterium engbaekii Copenhagen 20538 is a coccus-shaped human pathogen of the family Mycobacteriaceae.
NCBI tax id
- NCBI tax id: 188915
- Matching level: species
strain history
- @ref: 18059
- history: <- ATCC; ATCC 27353 <- T. Korsak <- H. Boisvert <- Copenhagen, Denmark 20538
doi: 10.13145/bacdive8349.20240916.9.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Mycobacteriales
- family: Mycobacteriaceae
- genus: Mycobacterium
- species: Mycobacterium engbaekii
- full scientific name: Mycobacterium engbaekii Tortoli et al. 2013
synonyms
- @ref: 20215
- synonym: Mycolicibacter engbaekii
@ref: 18059
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Mycobacteriaceae
genus: Mycobacterium
species: Mycobacterium engbaekii
full scientific name: Mycobacterium engbaekii Tortoli et al. 2013 emend. Nouioui et al. 2018
strain designation: Copenhagen 20538, Eng 1
type strain: yes
Morphology
cell morphology
| @ref | cell shape | gram stain | confidence | motility |
|---|---|---|---|---|
| 30561 | coccus-shaped | |||
| 69480 | positive | 91.63 | ||
| 69480 | 91.375 | no |
pigmentation
- @ref: 30561
- production: yes
Culture and growth conditions
culture medium
- @ref: 18059
- name: MIDDLEBROOK MEDIUM (DSMZ Medium 645)
- growth: yes
- link: https://mediadive.dsmz.de/medium/645
- composition: Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 18059 | positive | growth | 37 |
| 30561 | positive | growth | 25-37 |
| 30561 | positive | optimum | 31 |
Physiology and metabolism
spore formation
- @ref: 30561
- spore formation: no
metabolite utilization
- @ref: 30561
- Chebi-ID: 53426
- metabolite: tween 80
- utilization activity: +
- kind of utilization tested: carbon source
Safety information
risk assessment
- @ref: 18059
- pathogenicity human: yes, in single cases
- pathogenicity animal: yes, in single cases
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Mycobacterium engbaekii strain ATCC 27353 16S ribosomal RNA gene, partial sequence | JN571172 | 479 | nuccore | 188915 |
| 18059 | Mycobacterium engbaekii 16S ribosomal RNA gene, partial sequence | AF480577 | 1468 | nuccore | 188915 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Mycolicibacter engbaekii ATCC 27353 | GCA_002101585 | contig | ncbi | 188915 |
| 66792 | Mycobacterium engbaekii strain ATCC 27353 | 188915.3 | wgs | patric | 188915 |
| 66792 | Mycolicibacter engbaekii ATCC 27353 | 2865367387 | draft | img | 188915 |
Genome-based predictions
predictions
| @ref | trait | model | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 69481 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 86 | no |
| 69480 | gram-positive | gram-positive | Positive reaction to Gram-staining | yes | 91.63 | no |
| 69480 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | no | 94.589 | no |
| 69480 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 67.611 | yes |
| 69480 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | yes | 84.507 | no |
| 69480 | thermophilic | thermophile | Ability to grow at temperatures above or equal to 45°C | no | 94.9 | yes |
| 69480 | flagellated | motile2+ | Ability to perform flagellated movement | no | 91.375 | no |
External links
@ref: 18059
culture collection no.: DSM 45694, ATCC 27353
straininfo link
- @ref: 77751
- straininfo: 38954
literature
- topic: Phylogeny
- Pubmed-ID: 22447702
- title: Survey of 150 strains belonging to the Mycobacterium terrae complex and description of Mycobacterium engbaekii sp. nov., Mycobacterium heraklionense sp. nov. and Mycobacterium longobardum sp. nov.
- authors: Tortoli E, Gitti Z, Klenk HP, Lauria S, Mannino R, Mantegani P, Mariottini A, Neonakis I
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.038737-0
- year: 2012
- mesh: Bacterial Typing Techniques, DNA, Bacterial/genetics, Genes, Bacterial, Molecular Sequence Data, Mycolic Acids/analysis, Nontuberculous Mycobacteria/*classification/genetics, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
- topic2: Genetics
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed | ID_cross_reference |
|---|---|---|---|---|---|---|---|
| 18059 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45694) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-45694 | ||||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | ||
| 30561 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28776041 | 26892 | ||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | ||||
| 77751 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID38954.1 | StrainInfo: A central database for resolving microbial strain identifiers |