Strain identifier

BacDive ID: 8324

Type strain: Yes

Species: Mycobacterium smegmatis

Strain Designation: GA 735, Cornell 3, 14 133 0010

Strain history: CIP <- 1995, V. Vincent, Inst. Pasteur, Paris, France: strain 14 133 0010 <- 1981, ATCC <- NCTC <- R.E. Gordon <- Hagan <- Alvarez and Tavel: strain Cornell 3

NCBI tax ID(s): 1772 (species)

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General

@ref: 11178

BacDive-ID: 8324

DSM-Number: 43756

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, human pathogen

description: Mycobacterium smegmatis GA 735 is an aerobe, mesophilic, Gram-positive human pathogen of the family Mycobacteriaceae.

NCBI tax id

  • NCBI tax id: 1772
  • Matching level: species

strain history

@refhistory
11178<- NCTC; NCTC 8159 <- R. E. Gordon; Cornell 3
67771<- JCM <- NCTC <- R. Gordon <- Hagan <- Alvarez and Tavel (Cornell 3)
67770NCTC 8159 <-- R. E. Gordon <-- Hagan <-- Alvarez and Tavel strain Cornell 3.
122643CIP <- 1995, V. Vincent, Inst. Pasteur, Paris, France: strain 14 133 0010 <- 1981, ATCC <- NCTC <- R.E. Gordon <- Hagan <- Alvarez and Tavel: strain Cornell 3

doi: 10.13145/bacdive8324.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium smegmatis
  • full scientific name: Mycobacterium smegmatis (Trevisan 1889) Lehmann and Neumann 1899 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Bacillus smegmatis
    20215Mycolicibacterium smegmatis

@ref: 11178

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium smegmatis

full scientific name: Mycobacterium smegmatis (Trevisan 1889) Lehmann and Neumann 1899 emend. Nouioui et al. 2018

strain designation: GA 735, Cornell 3, 14 133 0010

type strain: yes

Morphology

cell morphology

@refgram stainmotilityconfidencecell shape
67771positive
69480no92.47
69480positive100
122643norod-shaped

multimedia

@refmultimedia contentintellectual property rightscaption
11178https://www.dsmz.de/microorganisms/photos/DSM_43756-1.jpg© Leibniz-Institut DSMZ
11178https://www.dsmz.de/microorganisms/photos/DSM_43756.jpg© Leibniz-Institut DSMZMedium 65 37°C

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11178GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
41725MEDIUM 55 - for Mycobacteriumyes
11178MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
122643CIP Medium 55yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55

culture temp

@refgrowthtypetemperaturerange
11178positivegrowth37mesophilic
41725positivegrowth37mesophilic
67770positivegrowth37mesophilic
67771positivegrowth37mesophilic
122643positivegrowth30-45
122643nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
67771aerobe
122643obligate aerobe

spore formation

@refspore formationconfidence
69481no90
69480no99.558

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1226434853esculin+hydrolysis
12264317632nitrate+reduction
12264316301nitrite-reduction
12264317632nitrate-respiration

metabolite production

  • @ref: 122643
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
122643oxidase-
122643beta-galactosidase+3.2.1.23
122643gelatinase-
122643amylase-
122643DNase+
122643caseinase-3.4.21.50
122643catalase+1.11.1.6
122643tween esterase+
122643lecithinase-
122643lipase+
122643urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122643-++++++++-++---++---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
122643++++++-------------+++-+--+++-+++----+-++--+---+++----++++--+------+--+-+--+---++-+-+---+-++-+-++--

Isolation, sampling and environmental information

isolation

  • @ref: 11178
  • geographic location: Plant Industry Station, Beltsville, Maryland
  • country: USA
  • origin.country: USA
  • continent: North America
  • latitude: 39.0358
  • longitude: -76.9175

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
11178yesyes2Risk group (German classification)
1226431Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mycobacterium smegmatis strain ATCC19420 16S ribosomal RNA (rrs) gene, partial sequenceAF059846175ena1772
20218Mycobacterium smegmatis 16S rRNA gene, strain ATCC 19420AJ1317611487ena1772
20218Mycobacterium smegmatis 16S rRNA geneAJ5360411461ena1772
20218Mycobacterium smegmatis strain ATCC 19420 16S ribosomal RNA gene, partial sequenceAY4570781487ena1772
20218Mycobacterium smegmatis strain DSM 43756 16S ribosomal RNA gene, partial sequenceKF4103561368ena1772
20218M.smegmatis (NCTC 8159) gene for 16S ribosomal RNA partial (207bp)X76255207ena1772
20218M.smegmatis (NCTC 8159) gene for 16S ribosomal RNA partial (238bp)X76256238ena1772
20218M.smegmatis (NCTC 8159) genes for 16S and 23s ribosomal RNA partial (381bp)X76257381ena1772

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycolicibacterium smegmatis NCTC8159GCA_001457595completencbi1772
66792Mycobacterium smegmatis strain NCTC81591772.19completepatric1772
66792Mycolicibacterium smegmatis NCTC81592657245626completeimg1772
66792Mycolicibacterium smegmatis ATCC 19420GCA_029222015contigncbi1772

GC content

  • @ref: 67770
  • GC-content: 67.3
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno90no
flagellatedno97.566no
gram-positiveyes88.284yes
anaerobicno99.238yes
aerobicyes87.686yes
halophileno88.506no
spore-formingno76.332no
glucose-utilyes90.83no
motileno90.757no
thermophileno99.311yes
glucose-fermentno89.281no

External links

@ref: 11178

culture collection no.: DSM 43756, ATCC 19420, JCM 5866, KCTC 9108, NCTC 8159, CCUG 21002, CCUG 21815, CECT 3020, CIP 104444, JCM 6386, NRRL B-14616, VKM Ac-1239

straininfo link

  • @ref: 77726
  • straininfo: 36980

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology8473863Cloning and sequencing of the gene which encodes the highly inducible acetamidase of Mycobacterium smegmatis.Mahenthiralingam E, Draper P, Davis EO, Colston MJJ Gen Microbiol10.1099/00221287-139-3-5751993Amidohydrolases/*genetics, Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Bacterial/genetics, *Genes, Bacterial, Molecular Sequence Data, Mycobacterium/*enzymology/*genetics, Oligonucleotide Probes, Open Reading Frames, Restriction MappingGenetics
Metabolism8557572Structure determination of ribosylated rifampicin and its derivative: new inactivated metabolites of rifampicin by mycobacterial strains.Morisaki N, Kobayashi H, Iwasaki S, Furihata K, Dabbs ER, Yazawa K, Mikami YJ Antibiot (Tokyo)10.7164/antibiotics.48.12991995*Antibiotics, Antitubercular/chemistry/metabolism, Biotransformation, Drug Resistance, Microbial, *Leprostatic Agents/chemistry/metabolism, Magnetic Resonance Spectroscopy, Molecular Structure, Mycobacterium/*metabolism, Ribose/chemistry/*metabolism, Rifampin/analogs & derivatives/*chemistry/*metabolism
Genetics9245815Regulation of the inducible acetamidase gene of Mycobacterium smegmatis.Parish T, Mahenthiralingam E, Draper P, Davis EO, Colston EOMicrobiology (Reading)10.1099/00221287-143-7-22671997Amidohydrolases/*genetics, Amino Acid Sequence, Base Sequence, Enzyme Induction/genetics, *Gene Expression Regulation, Bacterial, *Gene Expression Regulation, Enzymologic, Molecular Sequence Data, Mycobacterium/enzymology/*genetics, PlasmidsEnzymology
Metabolism9869590ADP-ribosylation as an intermediate step in inactivation of rifampin by a mycobacterial gene.Quan S, Imai T, Mikami Y, Yazawa K, Dabbs ER, Morisaki N, Iwasaki S, Hashimoto Y, Furihata KAntimicrob Agents Chemother10.1128/AAC.43.1.1811999ADP Ribose Transferases/genetics/*metabolism, Antibiotics, Antitubercular/*metabolism/pharmacology, Cloning, Molecular, Drug Resistance, Microbial, Escherichia coli/genetics, *Genes, Bacterial, Lac Operon, Magnetic Resonance Spectroscopy, Mycobacterium smegmatis/drug effects/genetics/*metabolism, Promoter Regions, Genetic, Rifampin/*metabolism/pharmacologyEnzymology
Enzymology10227476Pathogenicity of Mycobacterium fortuitum and Mycobacterium smegmatis to goldfish, Carassius auratus.Talaat AM, Trucksis M, Kane AS, Reimschuessel RVet Microbiol10.1016/s0378-1135(99)00002-41999Animals, Fish Diseases/*microbiology/pathology/physiopathology, Goldfish, Granuloma/microbiology/pathology/veterinary, Mycobacterium Infections, Nontuberculous/pathology/physiopathology/*veterinary, *Mycobacterium fortuitum/isolation & purification/pathogenicity, *Mycobacterium smegmatis/isolation & purification/pathogenicity, Peritoneum, Survival Analysis, VirulencePhylogeny
Phylogeny10555330Mycobacterium wolinskyi sp. nov. and Mycobacterium goodii sp. nov., two new rapidly growing species related to Mycobacterium smegmatis and associated with human wound infections: a cooperative study from the International Working Group on Mycobacterial Taxonomy.Brown BA, Springer B, Steingrube VA, Wilson RW, Pfyffer GE, Garcia MJ, Menendez MC, Rodriguez-Salgado B, Jost KC Jr, Chiu SH, Onyi GO, Bottger EC, Wallace RJ JrInt J Syst Bacteriol10.1099/00207713-49-4-14931999Adolescent, Adult, Aged, Aged, 80 and over, *Bacterial Proteins, Bacterial Typing Techniques, Base Composition, Base Sequence, Chaperonin 60, Chaperonins/genetics, Chromatography, High Pressure Liquid, DNA, Bacterial/chemistry/genetics, Female, Genes, rRNA/genetics, Humans, Male, Microbial Sensitivity Tests, Middle Aged, Molecular Sequence Data, Mycobacterium/*classification/genetics/isolation & purification/physiology, Mycobacterium Infections/*microbiology, Mycobacterium smegmatis/classification/genetics/isolation & purification, Nucleic Acid Hybridization, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Wound Infection/*microbiologyPathogenicity
Metabolism10647083Identification and characterization of a new intermediate in the ribosylative inactivation pathway of rifampin by Mycobacterium smegmatis.Imai T, Watanabe K, Mikami Y, Yazawa K, Ando A, Nagata Y, Morisaki N, Hashimoto Y, Furihata K, Dabbs ERMicrob Drug Resist10.1089/mdr.1999.5.2591999Antibiotics, Antitubercular/*metabolism, Microbial Sensitivity Tests, Molecular Sequence Data, Mycobacterium smegmatis/*metabolism, Ribose/*metabolism, Rifampin/*metabolismGenetics
Metabolism10819298Structures of ADP-ribosylated rifampicin and its metabolite: intermediates of rifampicin-ribosylation by Mycobacterium smegmatis DSM43756.Morisaki N, Hashimoto Y, Furihata K, Imai T, Watanabe K, Mikami Y, Yazawa K, Ando A, Nagata Y, Dabbs ERJ Antibiot (Tokyo)10.7164/antibiotics.53.2692000*ADP Ribose Transferases, Escherichia coli/enzymology/genetics, Magnetic Resonance Spectroscopy, Mycobacterium smegmatis/genetics/*metabolism, NAD/metabolism, Poly(ADP-ribose) Polymerases/genetics/*metabolism, Rifampin/*chemistry/*metabolismEnzymology
Pathogenicity11114922Characterization of In53, a class 1 plasmid- and composite transposon-located integron of Escherichia coli which carries an unusual array of gene cassettes.Naas T, Mikami Y, Imai T, Poirel L, Nordmann PJ Bacteriol10.1128/JB.183.1.235-249.20012001Amino Acid Sequence, Bacterial Proteins, Base Sequence, DNA Transposable Elements/*genetics, Drug Resistance, Microbial/genetics, Drug Resistance, Multiple/genetics, Escherichia coli/drug effects/*genetics, *Escherichia coli Proteins, Humans, Integrases/*genetics, Molecular Sequence Data, Plasmids/*genetics, *Recombination, Genetic, Sequence Analysis, DNAGenetics
Enzymology12547619Panniculitis, due to Mycobacterium smegmatis, in two Finnish cats.Alander-Damsten YK, Brander EE, Paulin LGJ Feline Med Surg10.1053/jfms.2001.01892003Animals, Cat Diseases/*diagnosis/pathology, Cats, Diagnosis, Differential, Female, Lumbosacral Region, Male, Mycobacterium Infections, Nontuberculous/complications/diagnosis/*veterinary, Mycobacterium smegmatis/*isolation & purification, Nipples, Panniculitis/diagnosis/etiology/*veterinaryPhylogeny
Genetics14506032A rifampin-hypersensitive mutant reveals differences between strains of Mycobacterium smegmatis and presence of a novel transposon, IS1623.Alexander DC, Jones JR, Liu JAntimicrob Agents Chemother10.1128/AAC.47.10.3208-3213.20032003Alleles, Amino Acid Sequence, Antibiotics, Antitubercular/*pharmacology, Bacterial Proteins/genetics, Base Sequence, DNA Transposable Elements/*genetics, Drug Resistance, Bacterial/genetics, Gene Library, Genes, Bacterial, Microbial Sensitivity Tests, Molecular Sequence Data, Mutation, Mycobacterium smegmatis/*drug effects/*genetics, Protein Biosynthesis/genetics, Rifampin/*pharmacology, Sequence Alignment, Sequence Homology, Nucleic Acid, Transposases/geneticsPathogenicity
Pathogenicity21489660New quinolin-4-yl-1,2,3-triazoles carrying amides, sulphonamides and amidopiperazines as potential antitubercular agents.Thomas KD, Adhikari AV, Chowdhury IH, Sumesh E, Pal NKEur J Med Chem10.1016/j.ejmech.2011.03.0392011Amides/*chemistry, Antitubercular Agents/chemical synthesis/chemistry/*pharmacology, Crystallography, X-Ray, Dose-Response Relationship, Drug, Escherichia coli/*drug effects/growth & development, Microbial Sensitivity Tests, Models, Molecular, Molecular Structure, Piperazines/*chemistry, Pseudomonas/*drug effects/growth & development, Quinolines/chemical synthesis/chemistry/*pharmacology, Stereoisomerism, Streptococcus/*drug effects/growth & development, Structure-Activity Relationship, Triazoles/chemical synthesis/chemistry/*pharmacology
Pathogenicity22253795Acanthamoeba polyphaga-enhanced growth of Mycobacterium smegmatis.Lamrabet O, Mba Medie F, Drancourt MPLoS One10.1371/journal.pone.00298332012Acanthamoeba/cytology/*microbiology/ultrastructure, Coculture Techniques, Endocytosis, Host-Parasite Interactions, Humans, Models, Biological, Mycobacterium Infections/microbiology, Mycobacterium smegmatis/*growth & development/ultrastructure, Trophozoites/cytology/microbiology/ultrastructure
Enzymology31378370Crystal structure and biochemical characterization of O-acetylhomoserine acetyltransferase from Mycobacterium smegmatis ATCC 19420.Sagong HY, Hong J, Kim KJBiochem Biophys Res Commun10.1016/j.bbrc.2019.07.1172019Acetyl Coenzyme A/*chemistry/metabolism, Acetyltransferases/*chemistry/genetics/metabolism, Amino Acid Sequence, Apoproteins/*chemistry/genetics/metabolism, Bacterial Proteins/*chemistry/genetics/metabolism, Catalytic Domain, Cloning, Molecular, Crystallography, X-Ray, Escherichia coli/genetics/metabolism, Gene Expression, Genetic Vectors/chemistry/metabolism, Haemophilus influenzae/chemistry/enzymology/genetics, Homoserine/*chemistry/metabolism, Kinetics, Leptospira interrogans/chemistry/enzymology/genetics, Models, Molecular, Mycobacteriaceae/chemistry/enzymology/genetics, Mycobacterium abscessus/chemistry/enzymology/genetics, Mycobacterium smegmatis/*chemistry/enzymology/genetics, Protein Binding, Protein Conformation, alpha-Helical, Protein Conformation, beta-Strand, Protein Interaction Domains and Motifs, Protein Multimerization, Recombinant Proteins/chemistry/genetics/metabolism, Sequence Alignment, Sequence Homology, Amino Acid, Substrate SpecificityMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11178Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43756)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43756
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41725Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16353
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77726Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID36980.1StrainInfo: A central database for resolving microbial strain identifiers
122643Curators of the CIPCollection of Institut Pasteur (CIP 104444)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104444