Strain identifier

BacDive ID: 8318

Type strain: No

Species: Mycobacterium smegmatis

Strain history: <- I. Tarnok, SN 2 <- Inst. Milchwirtschaft Kiel (M.lacticola)

NCBI tax ID(s): 1772 (species)

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General

@ref: 11039

BacDive-ID: 8318

DSM-Number: 43464

keywords: Bacteria, mesophilic, human pathogen

description: Mycobacterium smegmatis DSM 43464 is a mesophilic human pathogen that was isolated from phage host.

NCBI tax id

  • NCBI tax id: 1772
  • Matching level: species

strain history

  • @ref: 11039
  • history: <- I. Tarnok, SN 2 <- Inst. Milchwirtschaft Kiel (M.lacticola)

doi: 10.13145/bacdive8318.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium smegmatis
  • full scientific name: Mycobacterium smegmatis (Trevisan 1889) Lehmann and Neumann 1899 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Bacillus smegmatis
    20215Mycolicibacterium smegmatis

@ref: 11039

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium smegmatis

full scientific name: Mycobacterium smegmatis (Trevisan 1889) Lehmann and Neumann 1899 emend. Nouioui et al. 2018

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11039MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
11039LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354)yeshttps://mediadive.dsmz.de/medium/354Name: LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) Composition: Potato flour 18.6104 g/l L-Asparagin 2.23325 g/l KH2PO4 1.55087 g/l Mg-citrate 0.372208 g/l Malachite green 0.248139 g/l MgSO4 0.148883 g/l Glycerol Fresh egg mixture Distilled water

culture temp

  • @ref: 11039
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Isolation, sampling and environmental information

isolation

  • @ref: 11039
  • sample type: phage host

isolation source categories

  • Cat1: #Host
  • Cat2: #Microbial

Safety information

risk assessment

  • @ref: 11039
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

External links

@ref: 11039

culture collection no.: DSM 43464, ATCC 27204, IMET 10646, SN 2

straininfo link

  • @ref: 77720
  • straininfo: 38862

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny443992Interfamilial transfer of amber suppressor gene for the isolation of amber mutants of Mycobacteriophage I3.Ramakrishnan T, Shaila MSArch Microbiol10.1007/BF004230801979Drug Resistance, Microbial, Mutation, Mycobacteriophages/genetics/*isolation & purification, Mycobacterium/drug effects/*genetics, Pseudomonas/drug effects/*genetics, *Suppression, Genetic, Tetracycline/pharmacology, *Transformation, BacterialPathogenicity
Metabolism3440025Ribosomal RNA methylation in Mycobacterium smegmatis SN2.Srivastava R, Gopinathan KPBiochem Int1987Carbon Radioisotopes, Methylation, Mycobacterium/*metabolism, Phosphorus Radioisotopes, RNA, Ribosomal/isolation & purification/*metabolism, Ribonucleosides/analysisEnzymology
Enzymology3782054Arginyl-tRNA synthetase from Mycobacterium smegmatis SN2: purification and kinetic mechanism.Char S, Gopinathan KPJ Biochem10.1093/oxfordjournals.jbchem.a1217211986Acylation, Adenosine Monophosphate/analogs & derivatives/metabolism, Amino Acyl-tRNA Synthetases/*isolation & purification, Arginine-tRNA Ligase/*isolation & purification/metabolism, Kinetics, Magnesium/metabolism, Molecular Weight, Mycobacterium/*enzymology, Spermine/metabolismMetabolism
Enzymology3853966Purification and kinetic mechanism of lysyl-tRNA synthetase from Mycobacterium smegmatis SN2.Char S, Gopinathan KPBiochem Int1985Amino Acyl-tRNA Synthetases/*metabolism, Kinetics, Lysine-tRNA Ligase/isolation & purification/*metabolism, Molecular Weight, Mycobacterium/*enzymology, RNA, Transfer, Amino Acyl/metabolismMetabolism
Pathogenicity4793884Transduction of isoniazid susceptibility-resistance and streptomycin resistance in mycobacteria.Saroja D, Gopinathan KPAntimicrob Agents Chemother10.1128/AAC.4.6.6431973*Drug Resistance, Microbial, Isoniazid/*pharmacology, Mutation, Mycobacterium/*drug effects, Streptomycin/*pharmacology, *Transduction, Genetic
5014933Structure of a transducing mycobacteriophage.Kozloff LM, Sundar Raj CV, Rao RN, Chapman VA, DeLong SJ Virol10.1128/JVI.9.2.390-393.19721972Microscopy, Electron, *Mycobacteriophages, Mycobacterium, *Transduction, Genetic
Pathogenicity6667087Transfection of Mycobacterium smegmatis SN2 with mycobacteriophage I3 DNA.Karnik SS, Gopinathan KPArch Microbiol10.1007/BF004252161983Calcium Chloride/pharmacology, DNA, Viral/metabolism, Glycine/pharmacology, Mycobacteriophages/drug effects/*genetics/metabolism, Mycobacterium/drug effects/*genetics, Protamines/pharmacology, RNA, Fungal/pharmacology, Time Factors, *Transfection/drug effectsMetabolism
Metabolism7223123[Presence of histidine-degrading enzymes in mycobacteria and nocardia and reaction-kinetic studies on Mycobacterium smegmatis SN 2 (author's transl)].Rohrscheidt EZentralbl Bakteriol A1980Ammonia/metabolism, Ammonia-Lyases/*metabolism, Enzyme Activation, Histidine/metabolism, Histidine Ammonia-Lyase/antagonists & inhibitors/*metabolism, Hydrogen-Ion Concentration, Mycobacterium/*enzymology, Nocardia/*enzymology, Oxidation-Reduction, Species Specificity, TemperatureEnzymology
Enzymology9352074Characterization of a beta-lactamase from Mycobacterium smegmatis SN2.Basu D, Narayankumar DV, Van Beeumen J, Basu JBiochem Mol Biol Int10.1080/152165497002043611997Amino Acid Sequence, *Bacterial Proteins/antagonists & inhibitors/genetics/isolation & purification/metabolism, Clavulanic Acid/pharmacology, Enzyme Inhibitors/pharmacology, Molecular Sequence Data, Mycobacterium/*enzymology, Penicillanic Acid/analogs & derivatives/pharmacology, Sequence Alignment, Substrate Specificity, Sulbactam/pharmacology, beta-Lactam Resistance, beta-Lactamase Inhibitors, *beta-Lactamases/genetics/isolation & purification/metabolismPhylogeny
Metabolism15289559Genomic organization and in vivo characterization of proteolytic activity of FtsH of Mycobacterium smegmatis SN2.Anilkumar G, Srinivasan R, Ajitkumar PMicrobiology (Reading)10.1099/mic.0.27090-02004ATP-Dependent Proteases, Amino Acid Sequence, Bacterial Proteins/chemistry/*genetics/*metabolism, Cloning, Molecular, Escherichia coli/genetics/metabolism, Escherichia coli Proteins/genetics/metabolism, Gene Expression, Genes, Bacterial, Genetic Complementation Test, *Genome, Bacterial, Heat-Shock Proteins/metabolism, Membrane Proteins/chemistry/*genetics/*metabolism, Metalloproteases/chemistry/*genetics/*metabolism, Molecular Sequence Data, Mycobacterium smegmatis/*genetics/*metabolism, Protein Structure, Tertiary, Recombinant Proteins/chemistry/genetics/metabolism, SEC Translocation Channels, Sequence Homology, Amino Acid, Sigma Factor/metabolismGenetics
Pathogenicity22253795Acanthamoeba polyphaga-enhanced growth of Mycobacterium smegmatis.Lamrabet O, Mba Medie F, Drancourt MPLoS One10.1371/journal.pone.00298332012Acanthamoeba/cytology/*microbiology/ultrastructure, Coculture Techniques, Endocytosis, Host-Parasite Interactions, Humans, Models, Biological, Mycobacterium Infections/microbiology, Mycobacterium smegmatis/*growth & development/ultrastructure, Trophozoites/cytology/microbiology/ultrastructure

Reference

@idauthorscataloguedoi/urltitle
11039Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43464)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43464
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
77720Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID38862.1StrainInfo: A central database for resolving microbial strain identifiers