Strain identifier
BacDive ID: 8315
Type strain: 
Species: Mycobacterium smegmatis
Strain history: <- K. Kieslich, Schering 215 <- ATCC <- L.F. Bojalil, W-113 <- S. A. Waksman, 113 <- ATCC <- Hyg. Lab., Washington DC, 178 (Bacillus smegmatis)
NCBI tax ID(s): 1772 (species)
version 9 (current version)
General
@ref: 10921
BacDive-ID: 8315
DSM-Number: 43277
keywords: 16S sequence, Bacteria, human pathogen
description: Mycobacterium smegmatis DSM 43277 is a human pathogen of the family Mycobacteriaceae.
NCBI tax id
- NCBI tax id: 1772
- Matching level: species
strain history
- @ref: 10921
- history: <- K. Kieslich, Schering 215 <- ATCC <- L.F. Bojalil, W-113 <- S. A. Waksman, 113 <- ATCC <- Hyg. Lab., Washington DC, 178 (Bacillus smegmatis)
doi: 10.13145/bacdive8315.20240510.9
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Mycobacteriales
- family: Mycobacteriaceae
- genus: Mycobacterium
- species: Mycobacterium smegmatis
- full scientific name: Mycobacterium smegmatis (Trevisan 1889) Lehmann and Neumann 1899 (Approved Lists 1980)
synonyms
@ref synonym 20215 Bacillus smegmatis 20215 Mycolicibacterium smegmatis
@ref: 10921
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Mycobacteriaceae
genus: Mycobacterium
species: Mycobacterium smegmatis
full scientific name: Mycobacterium smegmatis (Trevisan 1889) Lehmann and Neumann 1899 emend. Nouioui et al. 2018
type strain: no
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 10921 | MIDDLEBROOK MEDIUM (DSMZ Medium 645) | yes | https://mediadive.dsmz.de/medium/645 | Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water |
| 10921 | LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) | yes | https://mediadive.dsmz.de/medium/354 | Name: LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) Composition: Potato flour 18.6104 g/l L-Asparagin 2.23325 g/l KH2PO4 1.55087 g/l Mg-citrate 0.372208 g/l Malachite green 0.248139 g/l MgSO4 0.148883 g/l Glycerol Fresh egg mixture Distilled water |
culture temp
- @ref: 10921
- growth: positive
- type: growth
- temperature: 37
Safety information
risk assessment
- @ref: 10921
- pathogenicity human: yes
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Mycobacterium smegmatis strain ATCC 14468 16S ribosomal RNA gene, partial sequence | JQ348119 | 206 | nuccore | 1772 |
| 20218 | M.smegmatis 16S ribosomal RNA gene | M59281 | 148 | nuccore | 1772 |
| 20218 | Mycobacterium smegmatis 16S rRNA gene | X52922 | 1454 | nuccore | 1772 |
External links
@ref: 10921
culture collection no.: DSM 43277, ATCC 101, ATCC 14468, NCIB 9953, NCTC 10265, TMC 1546
straininfo link
- @ref: 77717
- straininfo: 46517
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Pathogenicity | 228161 | Interaction between 30 S ribosomal components in a viomycin resistant mutant of Mycobacterium smegmatis. | Mizuguchi Y, Suga K, Yamada T | Microbiol Immunol | 10.1111/j.1348-0421.1979.tb00500.x | 1979 | Chemical Phenomena, Chemistry, Drug Resistance, Microbial, Kanamycin/pharmacology, Mycobacterium/*drug effects/genetics, Protein Biosynthesis, Recombination, Genetic, Ribosomes/*drug effects, Streptomycin/pharmacology, Viomycin/*pharmacology | |
| Enzymology | 464582 | Purification, properties, and cytotoxic effect of a bacteriocin from Mycobacterium smegmatis. | Saito H, Watanabe T, Tomioka H | Antimicrob Agents Chemother | 10.1128/AAC.15.4.504 | 1979 | Antineoplastic Agents, Bacteriocins/analysis/*isolation & purification, Chemical Phenomena, Chemistry, Molecular Weight, Mycobacterium/*analysis/drug effects | Phylogeny |
| Pathogenicity | 498138 | Effect of a bacteriocin produced by Mycobacterium smegmatis on growth of cultured tumor and normal cells. | Saito H, Watanabe T | Cancer Res | 1979 | Adsorption, Animals, Bacteriocins/metabolism/*pharmacology, Cell Division/*drug effects, Cell Line, Cell Membrane/metabolism, Cell Transformation, Neoplastic, Cricetinae, Humans, Mice, *Mycobacterium, Neoplasms, Experimental/*drug therapy, Rats, Tumor Virus Infections/drug therapy | Metabolism | |
| Metabolism | 1653167 | Calmodulin-like protein and the phospholipids of Mycobacterium smegmatis. | Burra SS, Reddy PH, Falah SM, Venkitasubramanian TA, Murthy PS | FEMS Microbiol Lett | 10.1016/0378-1097(91)90593-y | 1991 | Calcium/physiology, Calmodulin/*analysis/physiology, Cyclic AMP/analysis, Glucose/pharmacology, Mycobacterium/*analysis, Phospholipids/*analysis/metabolism | |
| Enzymology | 2667997 | Modulation of arginine decarboxylase activity from Mycobacterium smegmatis. Evidence for pyridoxal-5'-phosphate-mediated conformational changes in the enzyme. | Balasundaram D, Tyagi AK | Eur J Biochem | 10.1111/j.1432-1033.1989.tb14934.x | 1989 | Apoenzymes/metabolism, Arginine/metabolism, Carboxy-Lyases/isolation & purification/*metabolism, Chromatography, Gel, Dialysis, Electrophoresis, Polyacrylamide Gel, Escherichia coli/enzymology, Hydrogen-Ion Concentration, Kinetics, Molecular Weight, Mycobacterium/*enzymology, Protein Conformation/drug effects, Protein Denaturation, Pyridoxal Phosphate/metabolism/*pharmacology, Spectrophotometry | Metabolism |
| Enzymology | 3342242 | Purification and properties of aspartate transcarbamylase from Mycobacterium smegmatis. | Masood R, Venkitasubramanian TA | Biochim Biophys Acta | 10.1016/0167-4838(88)90014-3 | 1988 | Allosteric Regulation, Aspartate Carbamoyltransferase/*isolation & purification/metabolism, Enzyme Stability, Kinetics, Macromolecular Substances, Molecular Weight, Mycobacterium/*enzymology | Phylogeny |
| Enzymology | 3440089 | Role of various carbon and nitrogen sources in the regulation of enzymes of pyrimidine biosynthesis in Mycobacterium smegmatis TMC 1546. | Masood R, Venkitasubramanian TA | Ann Inst Pasteur Microbiol | 10.1016/0769-2609(87)90036-6 | 1987 | Amino Acids/pharmacology, Arginine/pharmacology, Aspartate Carbamoyltransferase/antagonists & inhibitors/metabolism, Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)/metabolism, Carbohydrates/pharmacology, Carboxylic Acids/pharmacology, Culture Media, Mycobacterium/*enzymology/metabolism, Ornithine/pharmacology, Pyrimidines/*biosynthesis | Metabolism |
| Enzymology | 3566963 | Ornithine transcarbamylase from Mycobacterium smegmatis ATCC 14468: purification, properties, and reaction mechanism. | Ahmad S, Bhatnagar RK, Venkitasubramanian TA | Biochem Cell Biol | 10.1139/o86-177 | 1986 | Amines/pharmacology, Arginine/pharmacology, Hydrogen-Ion Concentration, Kinetics, Molecular Weight, Mycobacterium/*enzymology, Ornithine/metabolism, Ornithine Carbamoyltransferase/antagonists & inhibitors/isolation & purification/*metabolism, Phenylglyoxal/pharmacology, Sulfhydryl Reagents/pharmacology, Valine/pharmacology | Metabolism |
| Pathogenicity | 6166835 | Effects of a bacteriocin from Mycobacterium smegmatis on BALB/3T3 and simian virus 40-transformed BALB/c mouse cells. | Saito H, Watanabe T | Microbiol Immunol | 10.1111/j.1348-0421.1981.tb00002.x | 1981 | Animals, Bacteriocins/*pharmacology, Cell Line, Cell Membrane/physiology, Cell Survival/*drug effects, *Cell Transformation, Neoplastic, *Cell Transformation, Viral, DNA/biosynthesis, Lectins/pharmacology, Mice, Mice, Inbred BALB C, Protein Biosynthesis, RNA/biosynthesis, Simian virus 40, Wheat Germ Agglutinins | |
| Metabolism | 6372572 | Growth and macromolecular synthesis of spheroplasts of Mycobacterium smegmatis ATCC 14468. | Murty MV, Venkitasubramanian TA | Ann Microbiol (Paris) | 1983 | Bacteriological Techniques, Cell Wall/metabolism, Culture Media, Models, Genetic, Mycobacterium/genetics/growth & development/*metabolism, Spheroplasts/*metabolism | Cultivation | |
| Metabolism | 6508073 | Influence of carbon and nitrogen sources on arginine biosynthesis in Mycobacterium smegmatis ATCC 14468. | Ahmad S, Bhatnagar RK, Venkitasubramanian TA | Ann Microbiol (Paris) | 10.1016/s0769-2609(84)80021-6 | 1984 | Arginine/*biosynthesis/metabolism, Asparagine/metabolism, Carbon, Culture Media, Mycobacterium/enzymology/growth & development/*metabolism, Nitrogen, Ornithine/metabolism | Cultivation |
| Metabolism | 6508074 | Turnover of phospholipids and glycerides of spheroplasts of Mycobacterium smegmatis ATCC 14468. | Murty MV, Venkitasubramanian TA | Ann Microbiol (Paris) | 10.1016/s0769-2609(84)80022-8 | 1984 | Carbon Radioisotopes, Cardiolipins/metabolism, Glycerides/*metabolism, Mycobacterium/*metabolism/ultrastructure, Palmitic Acid, Palmitic Acids/metabolism, Phospholipids/*metabolism, Spheroplasts/*metabolism | |
| Metabolism | 6516537 | Phospholipids and glycerides composition during spheroplasts formation of Mycobacterium smegmatis ATCC 14468. | Murty MV, Venkitasubramanian TA | Z Naturforsch C Biosci | 10.1515/znc-1984-9-1016 | 1984 | Carbon Radioisotopes, Glycerides/*metabolism, Kinetics, Mycobacterium/*physiology, Palmitic Acid, Palmitic Acids/metabolism, Phospholipids/*metabolism, Spheroplasts/physiology | Enzymology |
| Metabolism | 6826523 | Mycobacteriocins produced by rapidly growing mycobacteria are Tween-hydrolyzing esterases. | Saito H, Tomioka H, Watanabe T, Yoneyama T | J Bacteriol | 10.1128/jb.153.3.1294-1300.1983 | 1983 | Bacteriocins/*metabolism, Esterases/*metabolism, Mycobacterium/*metabolism, Oleic Acid, Oleic Acids/pharmacology, Polysorbates/*metabolism | |
| Enzymology | 6885719 | Purification and characterization of the tween-hydrolyzing esterase of Mycobacterium smegmatis. | Tomioka H | J Bacteriol | 10.1128/jb.155.3.1249-1259.1983 | 1983 | Chelating Agents/pharmacology, Esterases/isolation & purification/*metabolism, Hydrogen-Ion Concentration, Kinetics, Metals/pharmacology, Molecular Weight, Mycobacterium/*enzymology, Polysorbates/*metabolism, Substrate Specificity, Temperature | Metabolism |
| Metabolism | 7419493 | Characterization of binding sites for a bacteriocin produced by Mycobacterium smegmatis. | Watanabe T, Saito H | J Bacteriol | 10.1128/jb.144.1.428-431.1980 | 1980 | Amino Sugars/pharmacology, Bacteriocins/*metabolism, Binding Sites, Carbohydrates/pharmacology, Cell Membrane/metabolism, Cell Wall/metabolism, Glucose/pharmacology, Mycobacterium/*metabolism, Structure-Activity Relationship | |
| Metabolism | 7759478 | Inositol phosphate capping of the nonreducing termini of lipoarabinomannan from rapidly growing strains of Mycobacterium. | Khoo KH, Dell A, Morris HR, Brennan PJ, Chatterjee D | J Biol Chem | 10.1074/jbc.270.21.12380 | 1995 | Antigens, Bacterial/*chemistry/metabolism, Arabinose/chemistry, Carbohydrate Sequence, Deuterium Oxide, Hydrolysis, Inositol Phosphates/*analysis/metabolism, Lipopolysaccharides/*chemistry/metabolism, Mannose/chemistry, Molecular Sequence Data, Mycobacterium/*chemistry/growth & development/metabolism, Mycobacterium bovis/chemistry, Mycobacterium leprae/chemistry, Mycobacterium tuberculosis/chemistry, Oxidation-Reduction, Species Specificity, Spectrometry, Mass, Fast Atom Bombardment | Genetics |
| Pathogenicity | 18450455 | Synthesis of methyl 5-S-alkyl-5-thio-D-arabinofuranosides and evaluation of their antimycobacterial activity. | Sanki AK, Boucau J, Srivastava P, Adams SS, Ronning DR, Sucheck SJ | Bioorg Med Chem | 10.1016/j.bmc.2008.03.062 | 2008 | Amino Acid Sequence, *Arabinose/analogs & derivatives/chemical synthesis/pharmacology, Dose-Response Relationship, Drug, Escherichia coli/drug effects, Microbial Sensitivity Tests, Models, Molecular, Molecular Sequence Data, Mycobacterium smegmatis/*drug effects, Mycobacterium tuberculosis/*drug effects, Sequence Alignment, Structure-Activity Relationship, Sulfhydryl Compounds/*chemistry | Genetics |
| Pathogenicity | 18678487 | Synthesis of triazole-oxazolidinones via a one-pot reaction and evaluation of their antimicrobial activity. | Demaray JA, Thuener JE, Dawson MN, Sucheck SJ | Bioorg Med Chem Lett | 10.1016/j.bmcl.2008.07.087 | 2008 | Anti-Bacterial Agents/*chemical synthesis/*pharmacology, Bacillus subtilis/drug effects, Enterococcus faecalis/drug effects, Microbial Sensitivity Tests, Mycobacterium smegmatis/drug effects, Oxazolidinones/*chemical synthesis/*pharmacology, Triazoles/*chemical synthesis/*pharmacology | |
| Pathogenicity | 22960551 | An antimicrobial evaluation of plants used for the treatment of respiratory infections in rural Maputaland, KwaZulu-Natal, South Africa. | York T, van Vuuren SF, de Wet H | J Ethnopharmacol | 10.1016/j.jep.2012.08.038 | 2012 | Anti-Infective Agents/*pharmacology, Bacteria/drug effects, Cryptococcus neoformans/drug effects, *Medicine, African Traditional, Microbial Sensitivity Tests, Plant Extracts/*pharmacology, Plants, Medicinal, Respiratory Tract Infections/drug therapy, Rural Population, South Africa | |
| 24495981 | Biocompatible high performance hyperbranched epoxy/clay nanocomposite as an implantable material. | Barua S, Dutta N, Karmakar S, Chattopadhyay P, Aidew L, Buragohain AK, Karak N | Biomed Mater | 10.1088/1748-6041/9/2/025006 | 2014 | Animals, Anti-Infective Agents/chemistry, Biocompatible Materials/*chemistry, Candida albicans, Epoxy Compounds/chemistry, Escherichia coli, Male, Materials Testing, Microbial Sensitivity Tests, Microscopy, Electron, Scanning, Microscopy, Electron, Transmission, Mycobacterium smegmatis, Nanocomposites/*chemistry, Polymers/chemistry, *Prostheses and Implants, Rats, Rats, Wistar, Regeneration, Spectroscopy, Fourier Transform Infrared, Staphylococcus aureus, X-Ray Diffraction | ||
| Pathogenicity | 24786571 | Antibacterial activities of plants from Central Africa used traditionally by the Bakola pygmies for treating respiratory and tuberculosis-related symptoms. | Fomogne-Fodjo MC, Van Vuuren S, Ndinteh DT, Krause RW, Olivier DK | J Ethnopharmacol | 10.1016/j.jep.2014.04.032 | 2014 | Africa, Central, Anti-Bacterial Agents/isolation & purification/*pharmacology, Bacteria/drug effects, Cameroon, Ethnopharmacology, Medicine, African Traditional/methods, Microbial Sensitivity Tests, Mycobacterium/*drug effects, Phytotherapy/methods, Plant Extracts/*pharmacology, Plants, Medicinal/*chemistry, Respiratory Tract Infections/drug therapy/microbiology | Enzymology |
| Pathogenicity | 25915607 | Molecular Docking Studies of the Antitumoral Activity and Characterization of New Chalcone. | San-Martin A, Donoso V, Leiva S, Bacho M, Nunez S, Gutierrez M, Rovirosa J, Bailon-Moscoso N, Camacho SC, Aviles OM, Cazar ME | Curr Top Med Chem | 10.2174/1568026615666150427125033 | 2015 | Anti-Bacterial Agents/chemistry/pharmacology, Antineoplastic Agents, Phytogenic/chemistry/*pharmacology, Apiaceae/*chemistry, Bacteria/drug effects, Chalcones/*chemistry/*pharmacology, Free Radicals, Humans, Microbial Sensitivity Tests, Molecular Structure, Structure-Activity Relationship | |
| Pathogenicity | 26749825 | Antimicrobial Diterpenes from Azorella Species Against Gram-Positive Bacteria. | Donoso V, Bacho M, Nuinez S, Rovirosa J, San-Martin A, Leiva S | Nat Prod Commun | 2015 | Anti-Bacterial Agents/chemistry/*pharmacology, Apiaceae/*chemistry, Diterpenes/chemistry/*pharmacology, Gram-Positive Bacteria/drug effects, Microbial Sensitivity Tests, Molecular Structure, Plant Extracts/chemistry/*pharmacology | ||
| Pathogenicity | 29733942 | Terminalia laxiflora and Terminalia brownii contain a broad spectrum of antimycobacterial compounds including ellagitannins, ellagic acid derivatives, triterpenes, fatty acids and fatty alcohols. | Salih EYA, Julkunen-Tiitto R, Lampi AM, Kanninen M, Luukkanen O, Sipi M, Lehtonen M, Vuorela H, Fyhrquist P | J Ethnopharmacol | 10.1016/j.jep.2018.04.030 | 2018 | Anti-Bacterial Agents/*pharmacology, Ellagic Acid/pharmacology, Fatty Acids/pharmacology, Fatty Alcohols/pharmacology, Hydrolyzable Tannins/pharmacology, Medicine, African Traditional, Microbial Sensitivity Tests, Mycobacterium smegmatis/*drug effects/growth & development, Plant Extracts/*pharmacology, Plant Structures, Sudan, *Terminalia, Triterpenes/pharmacology | |
| Enzymology | 34624680 | Hydrolyzable tannins (ellagitannins), flavonoids, pentacyclic triterpenes and their glycosides in antimycobacterial extracts of the ethnopharmacologically selected Sudanese medicinal plant Combretum hartmannianum Schweinf. | Salih EYA, Julkunen-Tiitto R, Luukkanen O, Fahmi MKM, Fyhrquist P | Biomed Pharmacother | 10.1016/j.biopha.2021.112264 | 2021 | Anti-Bacterial Agents/isolation & purification/*pharmacology, *Combretum/chemistry, Ethnopharmacology, Flavonoids/isolation & purification/*pharmacology, Glycosides/isolation & purification/*pharmacology, Humans, Hydrolyzable Tannins/isolation & purification/*pharmacology, Microbial Sensitivity Tests, Mycobacterium smegmatis/*drug effects/growth & development, Pentacyclic Triterpenes/isolation & purification/*pharmacology, Phytochemicals/isolation & purification/*pharmacology, Plant Extracts/isolation & purification/*pharmacology, Sudan | Phylogeny |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 10921 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43277) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-43277 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 77717 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID46517.1 | StrainInfo: A central database for resolving microbial strain identifiers |