Strain identifier

BacDive ID: 8178

Type strain: Yes

Species: Psychrobacter urativorans

Strain Designation: McLean B-57

Strain history: CIP <- 1997, ACAM <- ATCC <- R.A. McLean: strain B-57, Micrococcus cryophilus

NCBI tax ID(s): 45610 (species)

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General

@ref: 5216

BacDive-ID: 8178

DSM-Number: 14009

keywords: genome sequence, 16S sequence, Bacteria, aerobe, psychrophilic, Gram-negative, oval-shaped

description: Psychrobacter urativorans McLean B-57 is an aerobe, psychrophilic, Gram-negative bacterium that was isolated from finished package of pork sausage from frozen meat.

NCBI tax id

  • NCBI tax id: 45610
  • Matching level: species

strain history

@refhistory
5216<- CCUG <- M. Kocur, CCM <- ATCC (Micrococcus cryophilus) <- R. C. Cleverdon <- R. A. McLean
123735CIP <- 1997, ACAM <- ATCC <- R.A. McLean: strain B-57, Micrococcus cryophilus

doi: 10.13145/bacdive8178.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Psychrobacter
  • species: Psychrobacter urativorans
  • full scientific name: Psychrobacter urativorans Bowman et al. 1996

@ref: 5216

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Psychrobacter

species: Psychrobacter urativorans

full scientific name: Psychrobacter urativorans Bowman et al. 1996

strain designation: McLean B-57

type strain: yes

Morphology

cell morphology

  • @ref: 123735
  • gram stain: negative
  • cell shape: oval-shaped
  • motility: no

colony morphology

  • @ref: 44820
  • incubation period: 2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5216OXOID NUTRIENT BROTH (DSMZ Medium 948)yeshttps://mediadive.dsmz.de/medium/948Name: OXOID NUTRIENT BROTH (DSMZ Medium 948) Composition: Nutrient broth 13.0 g/l Distilled water
34629MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
5216LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium381.pdf
123735CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5216positivegrowth20psychrophilic
34629positivegrowth20psychrophilic
44820positivegrowth22psychrophilic
123735positivegrowth5-30
123735nogrowth37mesophilic
123735nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
44820aerobe
123735obligate aerobe

halophily

  • @ref: 123735
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
123735citrate-carbon source16947
123735esculin-hydrolysis4853
123735glucose-fermentation17234
123735lactose-fermentation17716
123735nitrate-reduction17632
123735nitrite-reduction16301
123735sodium thiosulfate+builds gas from132112
123735glucose-degradation17234
123735nitrate-respiration17632

antibiotic resistance

  • @ref: 123735
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 123735
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12373515688acetoin-
12373517234glucose-

enzymes

@refvalueactivityec
123735oxidase+
123735beta-galactosidase-3.2.1.23
123735alcohol dehydrogenase-1.1.1.1
123735gelatinase-
123735amylase-
123735DNase-
123735caseinase-3.4.21.50
123735catalase+1.11.1.6
123735tween esterase-
123735gamma-glutamyltransferase+2.3.2.2
123735lecithinase-
123735lipase-
123735lysine decarboxylase-4.1.1.18
123735ornithine decarboxylase-4.1.1.17
123735phenylalanine ammonia-lyase-4.3.1.24
123735tryptophan deaminase-
123735urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123735-+++-+----++--------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123735-------------------------------------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123735-------------------------------------------------------------------------+-+---++--+----+-+++++--++

Isolation, sampling and environmental information

isolation

@refsample type
5216finished package of pork sausage from frozen meat
44820Finished package of pork sausage from frozen meat
123735Food, Pork, sausage

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Bottled, canned, packed food
#Engineered#Food production#Meat

taxonmaps

  • @ref: 69479
  • File name: preview.99_279.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_80;96_193;97_209;98_234;99_279&stattab=map
  • Last taxonomy: Psychrobacter
  • 16S sequence: AJ609555
  • Sequence Identity:
  • Total samples: 7634
  • soil counts: 925
  • aquatic counts: 3187
  • animal counts: 3350
  • plant counts: 172

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
52161Risk group (German classification)
1237351Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Psychrobacter uratovorans ATCC 15174 16S ribosomal RNA geneU461411485ena45610
20218Psychrobacter urativorans 16S rRNA gene, type strain DSM 14009TAJ6095551529ena45610
20218Psychrobacter urativorans 16S rRNA gene, partialAJ247265446ena45610

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Psychrobacter urativorans Psychrobacter urativorans ACAM534GCA_904846695contigncbi45610
66792Psychrobacter urativorans strain Psychrobacter urativorans ACAM53445610.23wgspatric45610

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveno98.345no
anaerobicno98.554yes
halophileyes69.703no
spore-formingno96.816no
glucose-utilno59.128no
aerobicyes89.48yes
flagellatedno90.565no
thermophileno99.707yes
motileno87.453no
glucose-fermentno92.801no

External links

@ref: 5216

culture collection no.: DSM 14009, ACAM 534, ATCC 15174, CCM 900, CCUG 4982, NCIB 11372, DSM 20429, CIP 105100, NCIMB 11372

straininfo link

  • @ref: 77581
  • straininfo: 36289

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity8863407Novel Psychrobacter species from Antarctic ornithogenic soils.Bowman JP, Cavanagh J, Austin JJ, Sanderson KInt J Syst Bacteriol10.1099/00207713-46-4-8411996Antarctic Regions, Base Composition, Base Sequence, DNA, Ribosomal/chemistry, Humans, Lipids/analysis, Molecular Sequence Data, Moraxella/chemistry/*classification/genetics, Phenotype, RNA, Ribosomal, 16S/genetics, *Soil MicrobiologyGenetics
Phylogeny19502339Psychrobacter fulvigenes sp. nov., isolated from a marine crustacean from the Sea of Japan.Romanenko LA, Tanaka N, Frolova GM, Mikhailov VVInt J Syst Evol Microbiol10.1099/ijs.0.007195-02009Animals, Bacterial Typing Techniques, Base Composition, Crustacea/*microbiology, DNA Gyrase/genetics, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Japan, Molecular Sequence Data, Nucleic Acid Hybridization, Oceans and Seas, Phenotype, Phylogeny, Polymerase Chain Reaction/methods, Psychrobacter/*classification/genetics/*isolation & purification/physiology, RNA, Ribosomal, 16S/genetics, Seawater, Sequence Analysis, DNA, Species SpecificityGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5216Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14009)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14009
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34629Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17081
44820Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 4982)https://www.ccug.se/strain?id=4982
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
77581Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID36289.1StrainInfo: A central database for resolving microbial strain identifiers
123735Curators of the CIPCollection of Institut Pasteur (CIP 105100)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105100