Strain identifier

BacDive ID: 8126

Type strain: Yes

Species: Acinetobacter baylyi

Strain Designation: B2

Strain history: CIP <- 2002, P. Kämpfer, Giessen Univ., Giessen, Germany: strain B2

NCBI tax ID(s): 1120924 (strain), 202950 (species)

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General

@ref: 5636

BacDive-ID: 8126

DSM-Number: 14961

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative

description: Acinetobacter baylyi B2 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from activated sludge.

NCBI tax id

NCBI tax idMatching level
202950species
1120924strain

strain history

@refhistory
5636<- P. Kämpfer, University Giessen, Germany; B2 <- A. Hoogenraad
421422002, P. Kämpfer, Univ., Giessen, Germany: strain B2
116768CIP <- 2002, P. Kämpfer, Giessen Univ., Giessen, Germany: strain B2

doi: 10.13145/bacdive8126.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Acinetobacter
  • species: Acinetobacter baylyi
  • full scientific name: Acinetobacter baylyi Carr et al. 2003

@ref: 5636

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Acinetobacter

species: Acinetobacter baylyi

full scientific name: Acinetobacter baylyi Carr et al. 2003

strain designation: B2

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.973
116768negativeoval-shapedno

colony morphology

  • @ref: 5636
  • type of hemolysis: gamma
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5636CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
5636TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
42142MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
116768CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5636positivegrowth30mesophilic
42142positivegrowth30mesophilic
58978positivegrowth30-37mesophilic
116768positivegrowth10-41
116768nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
58978aerobe
116768obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.992

halophily

@refsaltgrowthtested relationconcentration
116768NaClpositivegrowth0-4 %
116768NaClnogrowth6 %
116768NaClnogrowth8 %
116768NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea+hydrolysis
6836929016arginine-hydrolysis
6836927897tryptophan-energy source
6836917632nitrate-reduction
11676816947citrate+carbon source
11676817632nitrate-reduction
11676816301nitrite-reduction
11676817234glucose+/-degradation
11676817632nitrate-respiration

antibiotic resistance

  • @ref: 116768
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
11676835581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6836935581indole-
11676815688acetoin-
11676817234glucose-

enzymes

@refvalueactivityec
5636catalase+1.11.1.6
5636cytochrome-c oxidase-1.9.3.1
68369cytochrome oxidase-1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease+3.5.1.5
68369arginine dihydrolase-3.5.3.6
116768oxidase-
116768beta-galactosidase-3.2.1.23
116768gelatinase-
116768catalase+1.11.1.6
116768gamma-glutamyltransferase+2.3.2.2
116768urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116768-+++++----++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
5636--+-+---+-----+/-++++--
5636--+/--+---+-----+/-++++--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116768+------------------------------------------++----+++++--------+++--+--+--+-+++-++-------++++++-++-+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
5636activated sludgeVictoriaAustraliaAUSAustralia and Oceania
58978Activated sludge
116768Environment, Bendigo waste water treatment plantVictoriaAustraliaAUSAustralia and Oceania1995

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Waste
  • Cat3: #Activated sludge

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
56361Risk group (German classification)
1167681Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5636
  • description: Acinetobacter baylyi strain DSM14961 16S ribosomal RNA gene, partial sequence
  • accession: EF611407
  • length: 453
  • database: ena
  • NCBI tax ID: 1120924

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acinetobacter baylyi DSM 14961 = CIP 107474GCA_000368685scaffoldncbi1120924
66792Acinetobacter baylyi DSM 14961 = CIP 107474GCA_000621045scaffoldncbi1120924
66792Acinetobacter baylyi DSM 14961 = CIP 1074741120924.5wgspatric1120924
66792Acinetobacter baylyi DSM 14961 = CIP 1074741120924.3wgspatric1120924
66792Acinetobacter baylyi DSM 149612565956683draftimg1120924
66792Acinetobacter baylyi DSM 149612556921654draftimg1120924

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno92.384no
flagellatedno94.411no
gram-positiveno97.736no
anaerobicno98.709no
aerobicyes94.206no
halophileno80.839no
spore-formingno97.792no
thermophileno99.74yes
glucose-utilno51.412yes
glucose-fermentno89.135no

External links

@ref: 5636

culture collection no.: DSM 14961, CIP 107474, CCUG 50765, CCM 7195

straininfo link

  • @ref: 77533
  • straininfo: 113562

literature

  • topic: Phylogeny
  • Pubmed-ID: 12892111
  • title: Seven novel species of Acinetobacter isolated from activated sludge.
  • authors: Carr EL, Kampfer P, Patel BKC, Gurtler V, Seviour RJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.02486-0
  • year: 2003
  • mesh: Acinetobacter/*classification/genetics/*isolation & purification, Base Sequence, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, In Situ Hybridization, Fluorescence, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sewage/*microbiology, Terminology as Topic, Victoria
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
5636Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14961)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14961
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
42142Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4913
58978Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 50765)https://www.ccug.se/strain?id=50765
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77533Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID113562.1StrainInfo: A central database for resolving microbial strain identifiers
116768Curators of the CIPCollection of Institut Pasteur (CIP 107474)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107474