Strain identifier

BacDive ID: 7879

Type strain: Yes

Species: Dactylosporangium salmoneum

Strain Designation: FD 25647

Strain history: KCC A-0272 <-- T. Shomura <-- ATCC 31222 <-- Pfizer; FD 25647.

NCBI tax ID(s): 53361 (species)

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General

@ref: 11261

BacDive-ID: 7879

DSM-Number: 43910

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, motile

description: Dactylosporangium salmoneum FD 25647 is an aerobe, spore-forming, mesophilic bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 53361
  • Matching level: species

strain history

@refhistory
11261<- JCM <- KCC <- T. Shomura <- ATCC <- Chas. Pfizer & Co., FD 25647
67770KCC A-0272 <-- T. Shomura <-- ATCC 31222 <-- Pfizer; FD 25647.

doi: 10.13145/bacdive7879.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micromonosporales
  • family: Micromonosporaceae
  • genus: Dactylosporangium
  • species: Dactylosporangium salmoneum
  • full scientific name: Dactylosporangium salmoneum (ex Celmer et al. 1978) Kim et al. 2010

@ref: 11261

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micromonosporaceae

genus: Dactylosporangium

species: Dactylosporangium salmoneum

full scientific name: Dactylosporangium salmoneum (ex Celmer et al. 1978) Kim et al. 2010

strain designation: FD 25647

type strain: yes

Morphology

cell morphology

  • @ref: 29586
  • gram stain: positive
  • motility: yes

colony morphology

@refcolony colorincubation periodmedium used
19978Saffron yellow (1017)10-14 daysISP 2
19978Sun yellow (1037)10-14 daysISP 3
19978Beige (1011)10-14 daysISP 4
19978Sun yellow (1037)10-14 daysISP 5
19978Beige (1011)10-14 daysISP 6
19978Beige (1011)10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
19978noISP 2
19978noISP 3
19978noISP 4
19978noISP 5
19978noISP 6
19978noISP 7

pigmentation

  • @ref: 29586
  • production: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11261GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
11261N-Z-AMINE-MEDIUM (DSMZ Medium 554)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium554.pdf
19978ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19978ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19978ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19978ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19978ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19978ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
11261positivegrowth28mesophilic
19978positiveoptimum28mesophilic
29586positivegrowth20-37
29586positiveoptimum28mesophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
29586positivegrowth04-10alkaliphile
29586positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 29586
  • oxygen tolerance: aerobe

spore formation

  • @ref: 29586
  • spore formation: yes

compound production

@refcompound
11261polycyclic ether antibiotic
67770Antibiotic 44,161 (polycyclic ether)

halophily

  • @ref: 29586
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 1.5 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H8), MK-9(H6)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1997817234glucose+
1997822599arabinose+
1997817992sucrose+
1997818222xylose-
1997817268myo-inositol-
1997829864mannitol+
1997828757fructose+
1997826546rhamnose+
1997816634raffinose+
1997862968cellulose-
2958615963ribitol+carbon source
2958622599arabinose+carbon source
2958618403L-arabitol+carbon source
2958617057cellobiose+carbon source
2958616947citrate+carbon source
2958623652dextrin+carbon source
2958628757fructose+carbon source
2958628260galactose+carbon source
2958617754glycerol+carbon source
2958617716lactose+carbon source
2958625115malate+carbon source
2958617306maltose+carbon source
2958629864mannitol+carbon source
2958637684mannose+carbon source
2958628053melibiose+carbon source
2958617268myo-inositol+carbon source
2958617272propionate+carbon source
2958615361pyruvate+carbon source
2958616634raffinose+carbon source
2958626546rhamnose+carbon source
2958617814salicin+carbon source
2958627082trehalose+carbon source
2958618222xylose+carbon source
2958617632nitrate+reduction

metabolite production

  • @ref: 29586
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: yes

enzymes

@refvalueactivityec
29586gelatinase+
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19978+++-+----++++++++--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
11261soilJapanJapanJPNAsia
67770SoilJapanJPNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

  • @ref: 11261
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Dactylosporangium salmoneum gene for 16S ribosomal RNA, partial sequenceD869381466ena53361
20218D.salmoneum 16S rRNA geneX931951477ena53361
11261Dactylosporangium salmoneum strain NRRL B-16294 16S ribosomal RNA gene, partial sequenceFJ9736071433ena53361

GC content

@refGC-contentmethod
1126173thermal denaturation, midpoint method (Tm)
2958673

External links

@ref: 11261

culture collection no.: DSM 43910, ATCC 31222, IFO 14103, JCM 3272, KCC A-0272, NBRC 14103, NRRL B-16294, BCRC 12481, IMSNU 20067

straininfo link

  • @ref: 77315
  • straininfo: 39948

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny20688750Dactylosporangium luridum sp. nov., Dactylosporangium luteum sp. nov. and Dactylosporangium salmoneum sp. nov., nom. rev., isolated from soil.Kim BY, Stach JEM, Weon HY, Kwon SW, Goodfellow MInt J Syst Evol Microbiol10.1099/ijs.0.016451-02010DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Micromonosporaceae/*classification/genetics/*isolation & purification, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil MicrobiologyGenetics
Phylogeny21037030Dactylosporangium tropicum sp. nov., isolated from soil.Thawai C, Tanasupawat S, Kudo TInt J Syst Evol Microbiol10.1099/ijs.0.028829-02010Amino Acids/analysis, Bacterial Typing Techniques, Base Composition, Carbohydrates/analysis, Cell Wall/chemistry, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Micromonosporaceae/chemistry/*classification/genetics/*isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analysisGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
11261Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43910)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43910
19978Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM43910.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
29586Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604125978
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
77315Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID39948.1StrainInfo: A central database for resolving microbial strain identifiers