Strain identifier

BacDive ID: 7853

Type strain: Yes

Species: Asanoa siamensis

Strain Designation: PS7-2

Strain history: <- C Suriyachadkun, BCC; BCC 41921 <- N. Niemhom

NCBI tax ID(s): 926357 (species)

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General

@ref: 18204

BacDive-ID: 7853

DSM-Number: 45793

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, Gram-positive

description: Asanoa siamensis PS7-2 is a spore-forming, mesophilic, Gram-positive bacterium that was isolated from temperate peat swamp forest soil.

NCBI tax id

  • NCBI tax id: 926357
  • Matching level: species

strain history

  • @ref: 18204
  • history: <- C Suriyachadkun, BCC; BCC 41921 <- N. Niemhom

doi: 10.13145/bacdive7853.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micromonosporales
  • family: Micromonosporaceae
  • genus: Asanoa
  • species: Asanoa siamensis
  • full scientific name: Asanoa siamensis Niemhom et al. 2013

@ref: 18204

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micromonosporaceae

genus: Asanoa

species: Asanoa siamensis

full scientific name: Asanoa siamensis Niemhom et al. 2013

strain designation: PS7-2

type strain: yes

Morphology

cell morphology

@refgram stainmotilityconfidence
30564positiveno
69480no96.204
69480positive100

pigmentation

  • @ref: 30564
  • production: yes

multimedia

  • @ref: 18204
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_45793.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
18204GPHF-MEDIUM (DSMZ Medium 553)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium553.pdf
18204BHI MEDIUM (DSMZ Medium 215)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium215.pdf
18204TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium535.pdf
18204GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
18204positivegrowth28mesophilic
30564positivegrowth25-30mesophilic
30564positiveoptimum27.5mesophilic

culture pH

@refabilitytypepHPH range
30564positivegrowth06-12alkaliphile
30564positiveoptimum9

Physiology and metabolism

spore formation

@refspore formationconfidence
30564yes
69481yes99
69480yes100

halophily

@refsaltgrowthtested relationconcentration
30564NaClpositivegrowth0-2 %
30564NaClpositiveoptimum1 %

observation

  • @ref: 30564
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3056422599arabinose+carbon source
3056417057cellobiose+carbon source
3056428757fructose+carbon source
3056428260galactose+carbon source
3056417234glucose+carbon source
3056417754glycerol+carbon source
3056417716lactose+carbon source
3056417306maltose+carbon source
3056429864mannitol+carbon source
3056437684mannose+carbon source
3056428053melibiose+carbon source
3056416634raffinose+carbon source
3056426546rhamnose+carbon source
3056433942ribose+carbon source
3056417814salicin+carbon source
3056417992sucrose+carbon source
3056418222xylose+carbon source
3056417632nitrate+reduction

Isolation, sampling and environmental information

isolation

  • @ref: 18204
  • sample type: temperate peat swamp forest soil
  • geographic location: Phayao Province, Phu-Sang National Park
  • country: Thailand
  • origin.country: THA
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Forest
#Environmental#Terrestrial#Soil
#Environmental#Terrestrial#Wetland (Swamp)

Safety information

risk assessment

  • @ref: 18204
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 18204
  • description: Asanoa siamensis gene for 16S rRNA, partial sequence
  • accession: AB597931
  • length: 1440
  • database: ena
  • NCBI tax ID: 926357

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Asanoa siamensis NBRC 107932GCA_016862555contigncbi926357
66792Asanoa siamensis strain NBRC 107932926357.3wgspatric926357

GC content

@refGC-contentmethod
1820472.3high performance liquid chromatography (HPLC)
3056472.3

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes99no
motileno91.797yes
flagellatedno98.166yes
gram-positiveyes90.077yes
anaerobicno98.931no
halophileno93.185yes
spore-formingyes94.001yes
glucose-utilyes89.885yes
aerobicyes94.009no
thermophileno98.686no
glucose-fermentno90.631no

External links

@ref: 18204

culture collection no.: DSM 45793, BCC 41921, NBRC 107932

straininfo link

  • @ref: 77290
  • straininfo: 400444

literature

  • topic: Phylogeny
  • Pubmed-ID: 22328605
  • title: Asanoa siamensis sp. nov., isolated from soil from a temperate peat swamp forest.
  • authors: Niemhom N, Suriyachadkun C, Tamura T, Thawai C
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.038851-0
  • year: 2012
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Micromonosporaceae/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Thailand, Trees/microbiology, Vitamin K 2/analysis, Wetlands
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
18204Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45793)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45793
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30564Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2689528776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77290Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID400444.1StrainInfo: A central database for resolving microbial strain identifiers