Strain identifier

BacDive ID: 77

Type strain: Yes

Species: Roseomonas pecuniae

Strain Designation: N75

Variant: Isotype of BacDive ID 78

Strain history: CIP <- 2009, P. Morais, FCTUC, Coimbra Univ., Coimbra, Portugal: strain N75

NCBI tax ID(s): 693023 (species)

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General

@ref: 17963

BacDive-ID: 77

DSM-Number: 25268

keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, coccus-shaped, colony-forming, pigmented

description: Roseomonas pecuniae N75 is an obligate aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and has a pink pigmentation.

NCBI tax id

  • NCBI tax id: 693023
  • Matching level: species

strain history

@refhistory
376902009, P. Morais, FCTUC, Coimbra Univ., Coimbra, Portugal: strain N75
17963<- LMG <- P. V. Morais, Univ. Coimbra, Portugal; N75
67772Espirito Santo C. University of Coimbra
121646CIP <- 2009, P. Morais, FCTUC, Coimbra Univ., Coimbra, Portugal: strain N75

doi: 10.13145/bacdive77.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodospirillales
  • family: Acetobacteraceae
  • genus: Roseomonas
  • species: Roseomonas pecuniae
  • full scientific name: Roseomonas pecuniae Lopes et al. 2011

  • synonyms

    • @ref: 20215
    • synonym: Pararoseomonas pecuniae

@ref: 17963

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Acetobacteraceae

genus: Roseomonas

species: Roseomonas pecuniae

full scientific name: Roseomonas pecuniae Lopes et al. 2011

strain designation: N75

variant: Isotype of BacDive ID 78

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotility
22962negativecoccus-shapedno
121646negativeoval-shapedno

colony morphology

@refcolony colorcolony shapeincubation periodmedium used
22962pinkish redcircular2 daysR2A
22962pinkish redcircular2 daystryptic soy agar

pigmentation

  • @ref: 22962
  • production: yes
  • color: pink

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17963CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
22962Reasoner's 2A agar (R2A)yes
37690MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)

culture temp

@refgrowthtypetemperaturerange
17963positivegrowth28mesophilic
22962nogrowth5.0psychrophilic
22962nogrowth37.0mesophilic
22962positiveoptimum30.0mesophilic
37690positivegrowth30mesophilic
67772positiveminimum10psychrophilic
67772positiveoptimum30mesophilic
67772positivemaximum35mesophilic

culture pH

@refabilitytypepH
22962positiveoptimum7.0
67772positiveoptimum7.0

Physiology and metabolism

oxygen tolerance

  • @ref: 22962
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 22962
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
22962NaClnogrowth1.0 %(w/v)
22962NaClnogrowth2.0 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2296227613amygdalin-fermentation
2296218305arbutin-fermentation
2296215963ribitol-fermentation
2296217108D-arabinose-fermentation
2296217057cellobiose-fermentation
2296215824D-fructose-fermentation
2296212936D-galactose-fermentation
2296217634D-glucose-fermentation
2296217716lactose-fermentation
2296262318D-lyxose-fermentation
2296217306maltose-fermentation
2296216899D-mannitol-fermentation
2296216024D-mannose-fermentation
229626731melezitose-fermentation
2296228053melibiose-fermentation
2296216634raffinose-fermentation
2296216988D-ribose-fermentation
2296217992sucrose-fermentation
2296217924D-sorbitol-fermentation
2296216551D-trehalose-fermentation
2296232528turanose-fermentation
2296265327D-xylose-fermentation
2296216813galactitol-fermentation
2296217113erythritol-fermentation
229624853esculin-fermentation
2296228066gentiobiose-fermentation
2296217754glycerol-fermentation
2296228087glycogen-fermentation
2296217268myo-inositol-fermentation
2296215443inulin-fermentation
2296230849L-arabinose-fermentation
2296262345L-rhamnose-fermentation
2296217266L-sorbose-fermentation
2296265328L-xylose-fermentation
22962320061methyl alpha-D-glucopyranoside-fermentation
2296243943methyl alpha-D-mannoside-fermentation
2296274863methyl beta-D-xylopyranoside-fermentation
2296217814salicin-fermentation
2296228017starch-fermentation
2296217151xylitol-fermentation
22962167632-oxobutanoate-growth
22962309162-oxoglutarate-growth
22962370543-hydroxybutyrate-growth
22962182404-hydroxy-L-proline-growth
22962167244-hydroxybutyrate-growth
22962181014-hydroxyphenylacetic acid-growth
2296215963ribitol-growth
2296240585alpha-cyclodextrin-growth
2296217925alpha-D-glucose-growth
22962645522-hydroxybutyrate-growth
2296273706bromosuccinate-growth
2296227689decanoate-growth
2296217057cellobiose-growth
2296216383cis-aconitate-growth
2296216947citrate-growth
2296215570D-alanine-growth
2296218333D-arabitol-growth
2296215824D-fructose-growth
2296212936D-galactose-growth
229628391D-gluconate-growth
2296217784D-glucosaminic acid-growth
2296217634D-glucose-growth
2296215748D-glucuronate-growth
2296216899D-mannitol-growth
2296216024D-mannose-growth
2296217126DL-carnitine-growth
2296217113erythritol-growth
2296216000ethanolamine-growth
2296216865gamma-aminobutyric acid-growth
2296228066gentiobiose-growth
2296224265gluconate-growth
2296232323glucuronamide-growth
2296217754glycerol-growth
2296214336glycerol 1-phosphate-growth
2296217596inosine-growth
2296217240itaconate-growth
2296221217L-alaninamide-growth
2296216977L-alanine-growth
2296230849L-arabinose-growth
2296217196L-asparagine-growth
2296229991L-aspartate-growth
2296229985L-glutamate-growth
2296215971L-histidine-growth
2296215603L-leucine-growth
2296215729L-ornithine-growth
2296217295L-phenylalanine-growth
2296217203L-proline-growth
2296218183L-pyroglutamic acid-growth
2296217115L-serine-growth
2296224996lactate-growth
2296215792malonate-growth
2296217306maltose-growth
2296228053melibiose-growth
2296251850methyl pyruvate-growth
2296275146monomethyl succinate-growth
2296217268myo-inositol-growth
22962506227N-acetylglucosamine-growth
2296273784glycyl-l-glutamate-growth
2296218401phenylacetate-growth
2296232032potassium gluconate-growth
2296217148putrescine-growth
2296241865sebacic acid-growth
22962143136succinamate-growth
2296230031succinate-growth
2296227082trehalose-growth
2296253423tween 40-growth
2296253426tween 80-growth
2296216704uridine-growth
2296227248urocanic acid-growth
2296217151xylitol-growth
2296216199urea-hydrolysis
2296217521(-)-quinic acid+growth
2296217128adipate+growth
2296217306maltose+growth
2296223652dextrin+growth
2296262345L-rhamnose+growth
2296225115malate+growth
2296217272propionate+growth
2296217992sucrose+growth
229625291gelatin+hydrolysis
2296217632nitrate+reduction
12164617632nitrate+reduction
12164616301nitrite-reduction

metabolite production

  • @ref: 121646
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
22962alpha-chymotrypsin-3.4.21.1
22962cystine arylamidase-3.4.11.3
22962cytochrome oxidase-1.9.3.1
22962esterase (C 4)+
22962esterase lipase (C 8)+
22962leucine arylamidase-3.4.11.1
22962naphthol-AS-BI-phosphohydrolase+
22962trypsin-3.4.21.4
22962urease-3.5.1.5
22962valine arylamidase-
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
121646oxidase-
121646catalase+1.11.1.6
121646urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121646-+++----------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentenrichment cultureenrichment culture durationenrichment culture temperatureisolation proceduresampling dateisolation date
17963surface of copper-alloy coinPortugalPRTEurope
22962copper-alloy 50 Euro cent coinR2A5 days30.0purification by streaking on R2A
67772Copper-alloy 50 Euro cent coin collected from general circulationPortugalPRTEurope2007
121646Surface of a copper European coinPortugalPRTEurope2008

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Industrial production
#Engineered#Other#Currency

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
179631Risk group (German classification)
677721
1216461Risk group (French classification)

Sequence information

16S sequences

  • @ref: 17963
  • description: Roseomonas pecuniae strain N75 16S ribosomal RNA gene, partial sequence
  • accession: GU168019
  • length: 1441
  • database: ena
  • NCBI tax ID: 693023

GC content

  • @ref: 22962
  • GC-content: 72.8
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 17963

culture collection no.: DSM 25268, CIP 110074, LMG 25481, UCCCB34

straininfo link

  • @ref: 69761
  • straininfo: 364811

literature

  • topic: Phylogeny
  • Pubmed-ID: 20400668
  • title: Roseomonas pecuniae sp. nov., isolated from the surface of a copper-alloy coin.
  • authors: Lopes A, Espirito Santo C, Grass G, Chung AP, Morais PV
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.020966-0
  • year: 2010
  • mesh: Alloys, Base Composition, Cluster Analysis, Copper, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, *Environmental Microbiology, Fatty Acids/analysis, Methylobacteriaceae/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Numismatics, Phospholipids/analysis, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
17963Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25268)https://www.dsmz.de/collection/catalogue/details/culture/DSM-25268
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
22962André Lopes,Christophe Espirito Santo,Gregor Grass,Ana Paula Chung,Paula V. Morais10.1099/ijs.0.020966-0Roseomonas pecuniae sp. nov., isolated from the surface of a copper-alloy coinIJSEM 61: 610-615 201120400668
37690Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7890
67772Curators of the UCCCBhttps://ucccb.uc.pt/
68382Automatically annotated from API zym
69761Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID364811.1StrainInfo: A central database for resolving microbial strain identifiers
121646Curators of the CIPCollection of Institut Pasteur (CIP 110074)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110074