Strain identifier

BacDive ID: 7668

Type strain: No

Species: Micrococcus luteus

Strain Designation: 130.21

Strain history: CIP <- 1953, Y.A. Chabbert, Inst. Pasteur, Paris, France <- ATCC, Micrococcus flavus <- FDA, USA: strain PCI 1001, Sarcina lutea <- E.R. Squibb & Sons

NCBI tax ID(s): 1270 (species)

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General

@ref: 656

BacDive-ID: 7668

DSM-Number: 1790

keywords: Bacteria, obligate aerobe, mesophilic, Gram-positive, oval-shaped

description: Micrococcus luteus 130.21 is an obligate aerobe, mesophilic, Gram-positive bacterium that was isolated from Assay of bacitracin in body fluids.

NCBI tax id

  • NCBI tax id: 1270
  • Matching level: species

strain history

@refhistory
656<- ATCC <- Commercial Solvent Corp., 130.21 (Micrococcus flavus)
120705CIP <- 1953, Y.A. Chabbert, Inst. Pasteur, Paris, France <- ATCC, Micrococcus flavus <- FDA, USA: strain PCI 1001, Sarcina lutea <- E.R. Squibb & Sons

doi: 10.13145/bacdive7668.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Micrococcaceae
  • genus: Micrococcus
  • species: Micrococcus luteus
  • full scientific name: Micrococcus luteus (Schroeter 1872) Cohn 1872 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacteridium luteum

@ref: 656

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micrococcaceae

genus: Micrococcus

species: Micrococcus luteus

full scientific name: Micrococcus luteus (Schroeter 1872) Cohn 1872 emend. Nouioui et al. 2018

strain designation: 130.21

type strain: no

Morphology

cell morphology

  • @ref: 120705
  • gram stain: positive
  • cell shape: oval-shaped
  • motility: no

colony morphology

  • @ref: 120705
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
656NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
41170MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
120705CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
120705CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
656positivegrowth30mesophilic
41170positivegrowth30mesophilic
47536positivegrowth30mesophilic
120705positivegrowth22-41
120705nogrowth10psychrophilic
120705nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 120705
  • oxygen tolerance: obligate aerobe

antibiogram

@refmediumincubation temperatureincubation timeoxygen conditionPenicillin Goxacillinampicillinticarcillinmezlocillincefalotincefazolincefotaximeaztreonamimipenemtetracyclinechloramphenicolgentamycinamikacinvancomycinerythromycinlincomycinofloxacinnorfloxacincolistinpipemidic acidnitrofurantoinbacitracinpolymyxin bkanamycinneomycindoxycyclineceftriaxoneclindamycinfosfomycinmoxifloxacinlinezolidnystatinquinupristin/dalfopristinteicoplaninpiperacillin/tazobactam
656Mueller-Hinton Agar302aerob>5030>50>5042-44>50>50500>50>50>504040325048-5026-2816-1810084820343250>50>501838-40>500483040
656Mueller-Hinton Agar30aerob4224-264244-4636-384442-44400>50424032-3434-362836-383624-262214148-10402030-3230424042-44183242-4404228-3034-36
656Mueller-Hinton Agar42-442842504042-4446-48420>5040-4244-463034-363040-423826-2822-2416141040183028-30404040-4222-2432-3440-42042-443040
656Mueller-Hinton Agar302aerob>5028-3050>50>50>50>50500>50465038-40443050502820128046-481836-38344450>501240>50046-4828-3040
656Mueller-Hinton Agar374024404436404238-4005038-4038303226-28323222161406-83818262636n.d.n.d.n.d.n.d.n.d.n.d.n.d.n.d.n.d.
656Mueller Hinton30aerob502848>5042-4450>50500>5044483840-422846-4842-442616-181200422034-363242-44485016-183850044-462840-42
656Mueller Hinton30aerob>502650>5042>505048-500>5042464040-42305048-5026161000462034344450501040500463042

murein

  • @ref: 656
  • murein short key: A11. pep
  • type: A2 L-Lys peptide subunit

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
120705606565hippurate+hydrolysis
12070517632nitrate-reduction
12070516301nitrite-reduction

metabolite production

  • @ref: 120705
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 120705
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
120705oxidase-
120705beta-galactosidase+3.2.1.23
120705alcohol dehydrogenase-1.1.1.1
120705gelatinase+/-
120705amylase-
120705DNase-
120705caseinase-3.4.21.50
120705catalase+1.11.1.6
120705coagulase-
120705tween esterase-
120705gamma-glutamyltransferase-2.3.2.2
120705lecithinase-
120705lipase-
120705lysine decarboxylase-4.1.1.18
120705ornithine decarboxylase-4.1.1.17
120705phenylalanine ammonia-lyase-4.3.1.24
120705urease+3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    47536C14:00.514
    47536C15:00.315
    47536C16:0116
    47536C11:0 ANTEISO0.410.693
    47536C13:0 ANTEISO1.912.701
    47536C13:0 iso0.412.612
    47536C14:0 ISO4.213.618
    47536C15:0 ANTEISO75.214.711
    47536C15:0 ISO12.714.621
    47536C16:0 iso1.815.626
    47536C17:0 anteiso1.116.722
    47536C18:2 ω6,9c/C18:0 ANTE0.717.724
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120705-+++++----++---+----

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120705---------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

  • @ref: 47536
  • sample type: Assay of bacitracin in body fluids

isolation source categories

  • Cat1: #Host Body Product
  • Cat2: #Fluids

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
6561Risk group (German classification)
1207051Risk group (French classification)

Sequence information

GC content

  • @ref: 656
  • GC-content: 65.0

External links

@ref: 656

culture collection no.: DSM 1790, ATCC 10240, CCM 732, DSM 20490, IFO 3242, NBRC 3242, NCIB 8166, NCTC 7743, CCUG 21988, FDA 16, CIP 53.160, IMET 10759, NCDO 982, NCIMB 8166

straininfo link

  • @ref: 77111
  • straininfo: 13723

literature

Pubmed-IDtitleauthorsjournalDOIyearmeshtopictopic2
12396393Accelerated solvent extraction of animal feedingstuffs for microbial growth inhibition screening for the presence of antimicrobial feed additives.Higgins HC, McEvoy JDFood Addit Contam10.1080/026520302101450632002Animal Feed/*analysis, Animals, Anti-Bacterial Agents/analysis, Bacitracin/analysis, Drug Residues/*analysis, Drug Resistance, Microbial, False Negative Reactions, False Positive Reactions, Food Additives/*analysis, Glycopeptides, Sensitivity and Specificity, Solvents/chemistry, Spiramycin/analysis, Tylosin/analysis, Virginiamycin/analysis
16305671Antimicrobial activity of a nisin-activated plastic film for food packaging.Mauriello G, De Luca E, La Storia A, Villani F, Ercolini DLett Appl Microbiol10.1111/j.1472-765X.2005.01796.x2005Animals, Anti-Bacterial Agents/*pharmacology, Food Microbiology, Food Packaging/*methods, Food Preservation/*methods, Micrococcus luteus/*drug effects/growth & development, Milk/microbiology, Nisin/*pharmacologyBiotechnologyPathogenicity
17042189Development and in-house validation of a microbiological assay for determination of cefepime in injectable preparations.Souza MJ, Kulmann RR, Silva LM, Nogueira DR, Zimmermann ES, Schmidt CAJ AOAC Int2006Analysis of Variance, Anti-Bacterial Agents/administration & dosage/analysis/pharmacology/standards, Biological Assay/*methods/standards/statistics & numerical data, Cefepime, Cephalosporins/administration & dosage/*analysis/pharmacology/standards, Chromatography, Liquid, Injections, Mass Spectrometry, Micrococcus luteus/drug effects, Powders, Reference Standards, Reproducibility of ResultsPathogenicity
17657855A rapid and accurate 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay for quantification of bacteriocins with nisin as an example.Wang F, Cao LT, Hu SHJ Zhejiang Univ Sci B10.1631/jzus.2007.B05492007Bacteriocins/*analysis/metabolism, Colorimetry/*methods, Immunodiffusion, Micrococcus luteus/metabolism, *Nisin, Regression Analysis, Tetrazolium Salts/*analysis, Thiazoles/*analysis, Time FactorsMetabolism
18193752Development of a microbiological assay to determine the potency of ceftiofur sodium powder.Souza MJ, Rolim CM, Melo J, Souza Filho PS, Bergold AMJ AOAC Int2007Anti-Bacterial Agents/*analysis, Biological Assay, Cephalosporins/*analysis, Chromatography, Liquid, Indicators and Reagents, Micrococcus luteus/drug effects, Powders, Reproducibility of Results, SolutionsPathogenicity
21859126N-O chemistry for antibiotics: discovery of N-alkyl-N-(pyridin-2-yl)hydroxylamine scaffolds as selective antibacterial agents using nitroso Diels-Alder and ene chemistry.Wencewicz TA, Yang B, Rudloff JR, Oliver AG, Miller MJJ Med Chem10.1021/jm200794r2011Alkenes/chemical synthesis/chemistry, Anti-Bacterial Agents/*chemical synthesis/chemistry/pharmacology, Colony Count, Microbial, Crystallography, X-Ray, Drug Resistance, Bacterial, Gram-Positive Bacteria/drug effects, Hydroxylamines/*chemical synthesis/chemistry/pharmacology, Microbial Sensitivity Tests, Molecular Structure, Nitroso Compounds/*chemical synthesis/chemistry, Pyridines/*chemical synthesis/chemistry/pharmacology, Stereoisomerism, Structure-Activity RelationshipPathogenicity
22304453Synthesis and in vitro antimicrobial activity of new 4-phenyl-5-methyl-4H-1,2,4-triazole-3-thione derivatives.Popiolek L, Kosikowska U, Dobosz M, Malm AJ Enzyme Inhib Med Chem10.3109/14756366.2011.6533522012Anti-Infective Agents/*chemical synthesis/chemistry/*pharmacology, Chemistry Techniques, Synthetic, Cyclization, Drug Evaluation, Preclinical/methods, Gram-Positive Bacteria/drug effects, Microbial Sensitivity Tests, Micrococcus luteus/drug effects, Staphylococcus aureus/drug effects, Thiadiazoles/chemistry, Triazoles/chemistryPathogenicity
23603688Site-directed mutations in the lanthipeptide mutacin 1140.Chen S, Wilson-Stanford S, Cromwell W, Hillman JD, Guerrero A, Allen CA, Sorg JA, Smith LAppl Environ Microbiol10.1128/AEM.00704-132013Bacteriocins/*genetics/isolation & purification/pharmacology, Biotechnology/*methods, Clostridioides difficile/drug effects, Genes, Transgenic, Suicide/genetics, Genetic Vectors/genetics, Micrococcus luteus/drug effects, Molecular Structure, Mutagenesis, Site-Directed/*methods, Peptides/*genetics/isolation & purification/pharmacology, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Staphylococcus aureus/drug effects, Streptococcus/drug effects, Streptococcus mutans/*genetics/metabolismPathogenicityEnzymology
23710121Synthesis and antimicrobial evaluation of some novel 1,2,4-triazole and 1,3,4-thiadiazole derivatives.Popiolek L, Kosikowska U, Mazur L, Dobosz M, Malm AMed Chem Res10.1007/s00044-012-0302-92012
24374431Taguchi's experimental design for optimizing the production of novel thermostable polypeptide antibiotic from Geobacillus pallidus SAT4.Muhammad SA, Ahmed S, Ismail T, Hameed APak J Pharm Sci2014Anti-Bacterial Agents/*biosynthesis, Geobacillus/classification/*metabolism, Peptides/*metabolism, *Research DesignMetabolismBiotechnology
25229869Bactericidal activity of nukacin ISK-1: an alternative mode of action.Roy U, Islam MR, Nagao J, Iida H, Mahin AA, Li M, Zendo T, Nakayama J, Sonomoto KBiosci Biotechnol Biochem10.1080/09168451.2014.9184852014Anti-Bacterial Agents/isolation & purification/*pharmacology, Bacteria/drug effects, Bacteriocins/isolation & purification/*pharmacologyEnzymologyPhylogeny
28699251Antituberculotic activity of actinobacteria isolated from the rare habitats.Hussain A, Rather MA, Shah AM, Bhat ZS, Shah A, Ahmad Z, Parvaiz Hassan QLett Appl Microbiol10.1111/lam.127732017Actinobacteria/classification/genetics/*isolation & purification/*metabolism, Antitubercular Agents/chemistry/*metabolism/pharmacology, Bacillus subtilis/drug effects, Ecosystem, Escherichia coli/drug effects, Gas Chromatography-Mass Spectrometry, India, Klebsiella pneumoniae/drug effects, Microbial Sensitivity Tests, Phylogeny, Pseudomonas aeruginosa/drug effects, Soil MicrobiologyPhylogenyPathogenicity
30532629Identification and characterization of a novel antimicrobial peptide compound produced by Bacillus megaterium strain isolated from oral microflora.Al-Thubiani ASA, Maher YA, Fathi A, Abourehab MAS, Alarjah M, Khan MSA, Al-Ghamdi SBSaudi Pharm J10.1016/j.jsps.2018.05.0192018Phylogeny
32888099Intracellular biosynthesis of PbS quantum dots using Pseudomonas aeruginosa ATCC 27853: evaluation of antibacterial effects and DNA cleavage activities.Ocal N, Ceylan A, Duman FWorld J Microbiol Biotechnol10.1007/s11274-020-02917-z2020Anti-Bacterial Agents/*biosynthesis/chemistry/*pharmacology, Antifungal Agents/chemistry/pharmacology, Bacteria/drug effects, DNA Cleavage/*drug effects, Escherichia coli/drug effects, Fungi/drug effects, Lead/*chemistry, Microbial Sensitivity Tests, Microscopy, Electron, Transmission, Nanoparticles, Nanostructures/chemistry, Particle Size, Pseudomonas aeruginosa/*metabolism, Quantum Dots/chemistry/*metabolism, Spectrophotometry, Ultraviolet, Sulfides/*chemistry, X-Ray DiffractionPathogenicityMetabolism
33172144Rheological and Antimicrobial Properties of Chitosan and Quinoa Protein Filmogenic Suspensions with Thyme and Rosemary Essential Oils.Escamilla-Garcia M, Rios-Romo RA, Melgarejo-Mancilla A, Diaz-Ramirez M, Hernandez-Hernandez HM, Amaro-Reyes A, Pierro PD, Regalado-Gonzalez CFoods10.3390/foods91116162020
35265106Biosynthesis and Characterization of Silver Nanoparticles Produced by Phormidium ambiguum and Desertifilum tharense Cyanobacteria.Hanna AL, Hamouda HM, Goda HA, Sadik MW, Moghanm FS, Ghoneim AM, Alenezi MA, Alnomasy SF, Alam P, Elsayed TRBioinorg Chem Appl10.1155/2022/90725082022
36013417Inhibitory Effect of Bacillus licheniformis Strains Isolated from Canine Oral Cavity.Surin Hudakova N, Kacirova J, Sondorova M, Selianova S, Mucha R, Madar MLife (Basel)10.3390/life120812382022Phylogeny
36431827Antibacterial Properties of Crotoxin from Crotalus durissus terrificus-Insight into the Mechanism of Action.Nemecz D, Golinska PMolecules10.3390/molecules272277262022Animals, Humans, *Crotoxin/pharmacology, Crotalus/metabolism, Phospholipases A2/pharmacology, Anti-Bacterial Agents/pharmacology, Escherichia coli/metabolismPathogenicityMetabolism

Reference

@idauthorscataloguedoi/urltitle
656Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1790)https://www.dsmz.de/collection/catalogue/details/culture/DSM-1790
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
41170Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17044
47536Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 21988)https://www.ccug.se/strain?id=21988
68382Automatically annotated from API zym
77111Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13723.1StrainInfo: A central database for resolving microbial strain identifiers
120705Curators of the CIPCollection of Institut Pasteur (CIP 53.160)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2053.160