Strain identifier

BacDive ID: 7592

Type strain: Yes

Species: Paenarthrobacter nitroguajacolicus

Strain Designation: G2-1

Strain history: CIP <- 2004, CCM <- L. Kotouckova: strain G2-1

NCBI tax ID(s): 1303677 (strain), 211146 (species)

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General

@ref: 5771

BacDive-ID: 7592

DSM-Number: 15232

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, motile, rod-shaped

description: Paenarthrobacter nitroguajacolicus G2-1 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from polluted soil.

NCBI tax id

NCBI tax idMatching level
211146species
1303677strain

strain history

@refhistory
5771<- L. Kotoucková; G2-1
67770CCM 4924 <-- L. Kotou?ková G2-1.
121313CIP <- 2004, CCM <- L. Kotouckova: strain G2-1

doi: 10.13145/bacdive7592.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Micrococcaceae
  • genus: Paenarthrobacter
  • species: Paenarthrobacter nitroguajacolicus
  • full scientific name: Paenarthrobacter nitroguajacolicus (Kotou?ková et al. 2004) Busse 2016

  • synonyms

    • @ref: 20215
    • synonym: Arthrobacter nitroguajacolicus

@ref: 5771

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micrococcaceae

genus: Paenarthrobacter

species: Paenarthrobacter nitroguajacolicus

full scientific name: Paenarthrobacter nitroguajacolicus (Kotou?ková et al. 2004) Busse 2016

strain designation: G2-1

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
30089positive2.5 µm0.8 µmrod-shapedyes
121313positiverod-shapedno

colony morphology

@refcolony colorincubation periodmedium used
18521Beige (1001)10-14 daysISP 2
18521Beige (1001)10-14 daysISP 3
18521Beige (1001)10-14 daysISP 4
18521Beige (1001)10-14 daysISP 5
18521Beige (1001)10-14 daysISP 6
18521Beige (1001)10-14 daysISP 7
121313

multicellular morphology

@refforms multicellular complexmedium name
18521noISP 2
18521noISP 3
18521noISP 4
18521noISP 5
18521noISP 6
18521noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5771TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
18521ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18521ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18521ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18521ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18521ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18521ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
37401MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
121313CIP Medium 464yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=464
121313CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
18521positiveoptimum28mesophilic
5771positivegrowth28mesophilic
30089positivegrowth04-37
30089positiveoptimum27.5mesophilic
37401positivegrowth30mesophilic
67770positivegrowth28mesophilic
121313positivegrowth10-37
121313nogrowth41thermophilic
121313nogrowth45thermophilic

culture pH

@refabilitytypepH
30089positivegrowth06-08
30089positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
30089aerobe
121313obligate aerobe

spore formation

  • @ref: 30089
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
30089NaClpositivegrowth0-6 %
30089NaClpositiveoptimum3 %
121313NaClpositivegrowth0-6 %
121313NaClnogrowth8 %
121313NaClnogrowth10 %

murein

  • @ref: 5771
  • murein short key: A11.17
  • type: A3alpha L-Lys-L-Ala-L-Thr-L-Ala

observation

  • @ref: 67770
  • observation: quinones: MK-9(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1852162968cellulose+
1852116634raffinose+
1852126546rhamnose+
1852128757fructose+
1852129864mannitol+
1852117268myo-inositol+
1852118222xylose-
1852117992sucrose+
1852122599arabinose+
1852117234glucose+
3008930089acetate+carbon source
3008916449alanine+carbon source
3008922653asparagine+carbon source
3008917057cellobiose+carbon source
3008923652dextrin+carbon source
3008928757fructose+carbon source
3008928260galactose+carbon source
300895291gelatin+carbon source
3008929987glutamate+carbon source
3008917754glycerol+carbon source
3008928087glycogen+carbon source
3008925115malate+carbon source
3008917306maltose+carbon source
3008929864mannitol+carbon source
3008937684mannose+carbon source
3008951850methyl pyruvate+carbon source
3008917272propionate+carbon source
3008917148putrescine+carbon source
3008915361pyruvate+carbon source
3008916634raffinose+carbon source
3008933942ribose+carbon source
3008917822serine+carbon source
3008930911sorbitol+carbon source
3008917992sucrose+carbon source
300894853esculin+hydrolysis
12131316947citrate-carbon source
1213134853esculin+hydrolysis
121313606565hippurate-hydrolysis
12131317632nitrate-reduction
12131316301nitrite-reduction
12131317632nitrate-respiration
683794853esculin+hydrolysis
6837916199urea+hydrolysis
683795291gelatin-hydrolysis
6837917992sucrose+fermentation

antibiotic resistance

  • @ref: 121313
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 121313
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12131315688acetoin-
12131317234glucose-

enzymes

@refvalueactivityec
30089acid phosphatase+3.1.3.2
30089alkaline phosphatase+3.1.3.1
30089alpha-galactosidase+3.2.1.22
30089catalase+1.11.1.6
30089gelatinase+
30089cytochrome oxidase+1.9.3.1
68382alpha-mannosidase+3.2.1.24
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase (C 4)+
68379gelatinase-
68379urease+3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase+3.5.1.B15
121313oxidase-
121313beta-galactosidase+3.2.1.23
121313alcohol dehydrogenase-1.1.1.1
121313gelatinase+
121313amylase+
121313DNase+
121313caseinase+3.4.21.50
121313catalase+1.11.1.6
121313tween esterase-
121313gamma-glutamyltransferase+2.3.2.2
121313lecithinase-
121313lipase-
121313lysine decarboxylase-4.1.1.18
121313ornithine decarboxylase-4.1.1.17
121313phenylalanine ammonia-lyase-4.3.1.24
121313tryptophan deaminase-
121313urease-3.5.1.5

API coryne

@refPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELSAC
18521+++----++-+

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18521-++-+--+---++-+++++
121313++--+--+----+-++-+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121313+++++-++++++++++++++++++-+-++--++++++------+--+--+-+-++-+--++++++-+-++-+++++---++-----+-+++++++-+++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
5771polluted soilBrno, ChrliceCzech RepublicCZEEurope
67770Forest soil
121313Environment, SoilCzech RepublicCZEEurope2000

isolation source categories

Cat1Cat2Cat3
#Engineered#Contamination
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_3469.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_99;97_138;98_2663;99_3469&stattab=map
  • Last taxonomy: Paenarthrobacter
  • 16S sequence: AJ512504
  • Sequence Identity:
  • Total samples: 10568
  • soil counts: 3607
  • aquatic counts: 855
  • animal counts: 3936
  • plant counts: 2170

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
185211Hazard group
57711Risk group (German classification)
1213131Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5771
  • description: Arthrobacter nitroguaiacolicus partial 16S rRNA gene, strain CCM 4924T
  • accession: AJ512504
  • length: 1488
  • database: ena
  • NCBI tax ID: 211146

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Arthrobacter nitroguajacolicus JCM 141151303677.3wgspatric1303677
66792Paenarthrobacter nitroguajacolicus JCM 141152734481943draftimg1303677
67770Paenarthrobacter nitroguajacolicus JCM 14115GCA_001313365contigncbi1303677

GC content

@refGC-contentmethod
577161.9
6777061.9high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno88.7no
gram-positiveyes93.544no
anaerobicno99.385no
halophileno87.731no
spore-formingno83.509yes
thermophileno98.836yes
glucose-utilyes86.718no
aerobicyes96.925no
flagellatedno97.663no
glucose-fermentno85.314no

External links

@ref: 5771

culture collection no.: DSM 15232, CCM 4924, JCM 14115, CIP 108435, IAM 15314

straininfo link

  • @ref: 77037
  • straininfo: 132011

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15143023Arthrobacter nitroguajacolicus sp. nov., a novel 4-nitroguaiacol-degrading actinobacterium.Kotouckova L, Schumann P, Durnova E, Sproer C, Sedlacek I, Neca J, Zdrahal Z, Nemec MInt J Syst Evol Microbiol10.1099/ijs.0.02923-02004Amino Acid Sequence, Arthrobacter/*classification/genetics/isolation & purification/*metabolism, Base Composition, Biodegradation, Environmental, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Guaiacol/analogs & derivatives/metabolism, Microscopy, Electron, Scanning, Molecular Sequence Data, Peptidoglycan/genetics, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Soil MicrobiologyMetabolism
Phylogeny24925596Arthrobacter bambusae sp. nov., isolated from soil of a bamboo grove.Park Y, Kook M, Ngo HTT, Kim KY, Park SY, Mavlonov GT, Yi THInt J Syst Evol Microbiol10.1099/ijs.0.064550-02014Arthrobacter/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Bambusa/*microbiology, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
5771Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15232)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15232
18521Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM15232.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30089Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2644628776041
37401Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6057
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
77037Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID132011.1StrainInfo: A central database for resolving microbial strain identifiers
121313Curators of the CIPCollection of Institut Pasteur (CIP 108435)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108435