Strain identifier

BacDive ID: 7582

Type strain: Yes

Species: Glutamicibacter nicotianae

Strain Designation: NANDA-189

Strain history: LMG 16219 <-- ATCC 33408 <-- NCIB 10583 <-- K. Nand A-189.

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 4824

BacDive-ID: 7582

DSM-Number: 12798

keywords: Bacteria, 16S sequence, genome sequence, mesophilic, amino acid production

description: Glutamicibacter nicotianae NANDA-189 is a mesophilic bacterium that produces amino acids and was isolated from sewage.

NCBI tax id

NCBI tax idMatching level
1216975strain
257984species

strain history

doi: 10.13145/bacdive7582.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Micrococcaceae
  • genus: Glutamicibacter
  • species: Glutamicibacter nicotianae
  • full scientific name: Glutamicibacter nicotianae (Giovannozzi-Sermanni 1959) Busse 2016
  • synonyms

    @refsynonym
    20215Corynebacterium liquefaciens
    20215Glutamicibacter mysorens
    20215Brevibacterium liquefaciens
    20215Arthrobacter nicotianae
    20215Arthrobacter mysorens

@ref: 4824

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micrococcaceae

genus: Glutamicibacter

species: Glutamicibacter mysorens

full scientific name: Glutamicibacter mysorens (Nand and Rao 1972) Busse 2016 emend. Nouioui et al. 2018

strain designation: NANDA-189

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
18370Sand yellow (1002)10-14 daysISP 2
18370Zinc yellow (1018)10-14 daysISP 3
18370Ivory (1014)10-14 daysISP 4
18370Colorless10-14 daysISP 5
18370Honey yellow (1005)10-14 daysISP 6
18370Ivory (1014)10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
18370noISP 2
18370noISP 3
18370noISP 4
18370noISP 5
18370noISP 6
18370noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4824OXOID NUTRIENT BROTH (DSMZ Medium 948)yeshttps://bacmedia.dsmz.de/medium/948Name: OXOID NUTRIENT BROTH (DSMZ Medium 948) Composition: Nutrient broth 13.0 g/l Distilled water
4824RAE MEDIUM (DSMZ Medium 848)yeshttps://bacmedia.dsmz.de/medium/848Name: RAE MEDIUM (DSMZ Medium 848) Composition: Glucose 40.0 g/l Yeast extract 10.0 g/l Peptone 10.0 g/l Na2HPO4 x 2 H2O 3.38 g/l Citric acid x H2O 1.5 g/l Glacial acetic acid Absolute ethanol Distilled water
18370ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18370ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
18370ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18370ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18370ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18370ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
37402MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)

culture temp

@refgrowthtypetemperaturerange
18370positiveoptimum28mesophilic
4824positivegrowth28mesophilic
37402positivegrowth30mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

compound production

  • @ref: 4824
  • compound: L glutamic acid

halophily

  • @ref: 18370
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 7.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1837017234glucose+
1837022599arabinose-
1837017992sucrose+
1837017268myo-inositol+
1837029864mannitol-
1837028757fructose-
1837026546rhamnose-
1837016634raffinose+
1837062968cellulose-
6837928087glycogen-fermentation
6837917992sucrose+fermentation
6837917716lactose-fermentation
6837917306maltose+fermentation
6837916899D-mannitol+fermentation
6837965327D-xylose-fermentation
6837916988D-ribose-fermentation
6837917634D-glucose-fermentation
683795291gelatin+hydrolysis
6837916199urea+hydrolysis
683794853esculin-hydrolysis
6837917632nitrate+reduction
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno
6777029985L-glutamateyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68379gelatinase+
68379urease+3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase+3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase+3.5.1.B15
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18370+++-++++-++---++-+-

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
18370+++-++++-++---++-+-

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18370---------++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
4824sewageMysoreIndiaINDAsia
67770SewageMysore CityIndiaINDAsia

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Waste
  • Cat3: #Wastewater

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
183701
48241Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Arthrobacter mysorens partial 16S rRNA gene, type strain DSM 12798TAJ6174821491ena257984
20218Arthrobacter mysorens partial 16S rRNA gene, type strain LMG 16219TAJ6398311478ena257984

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Glutamicibacter mysorens DSM 12798GCA_002797775contigncbi257984
66792Glutamicibacter mysorens NBRC 103060 NBRC 103060GCA_001552115contigncbi1216975
66792Glutamicibacter mysorens NBRC 1030601216975.4wgspatric1216975
66792Glutamicibacter mysorens strain DSM 12798257984.4wgspatric257984
66792Glutamicibacter mysorens NBRC 1030602681812973draftimg1216975
66792Glutamicibacter mysorens DSM 127982627854287draftimg257984
67770Glutamicibacter mysorens NBRC 103060, whole genome shotgun sequencing projectBCQO00000000ncbi1216975
67770Glutamicibacter mysorens strain DSM 12798, whole genome shotgun sequencing projectPGEY00000000ncbi257984

GC content

  • @ref: 67770
  • GC-content: 62
  • method: genome sequence analysis

External links

@ref: 4824

culture collection no.: DSM 12798, ATCC 33408, NCIMB 10583, JCM 11565, CIP 102716, IAM 14869, KCTC 3381, LMG 16219, NBRC 103060, VTT E-072694

straininfo link

@refpassport
20218http://www.straininfo.net/strains/301934
20218http://www.straininfo.net/strains/33377
20218http://www.straininfo.net/strains/33379

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4824Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12798)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12798
18370Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM12798.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37402Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14433
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym