Glutamicibacter nicotianae (DSM 12798, ATCC 33408, NCIMB 10583)

Name: Glutamicibacter nicotianae (DSM 12798, ATCC 33408, NCIMB 10583)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7582

Type strain

Strain Designation NANDA-189, A-189

Culture col. no. DSM 12798 ATCC 33408 NCIMB 10583 NANDA-189 VTT E-072694 7 more

NCBI tax ID(s) 1216975 257984

Special Collections DSMZ JCM KCTC CIP Wink compendium

History <- NCIMB <- K. Nand LMG 16219 <-- ATCC 33408 <-- NCIB 10583 <-- K. Nand A-189. CIP <- 1987, NCIB <- K. Nand: strain A-189

Links

Glutamicibacter nicotianae NANDA-189 is an obligate aerobe, mesophilic, Gram-positive prokaryote that produces amino acids and was isolated from sewage.

amino acid production Gram-positive ovoid-shaped obligate aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 8180

LMG 16219,ATCC 33408,NCIMB 10583,JCM 11565,NCIB 10583,CIP 102716,DSM 12798,KCTC 3381,IAM 14869,NBRC 103060,VTT E-072694

@ref 20215

Last LPSN update 2026-02-09 11:19:30

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Micrococcaceae

Genus Glutamicibacter

Species Glutamicibacter nicotianae

Full scientific name Glutamicibacter nicotianae (Giovannozzi-Sermanni 1959) Busse 2016

Synonyms (5)

Corynebacterium liquefaciens
Glutamicibacter mysorens
Arthrobacter mysorens
Brevibacterium liquefaciens
Arthrobacter nicotianae

BacDive ID	Other strains from **Glutamicibacter nicotianae** (4)	Type strain
7524	G. nicotianae 94, CCUG 23842, DSM 20123, ATCC 15236, CCM ... (type strain)
7525	G. nicotianae DSM 20579, ATCC 14929, NCIB 9545, CCUG 43493, ...
101076	G. nicotianae ST033369(HKI),
161548	G. nicotianae JCM 1487

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
121215	positive	ovoid-shaped
125438			92
125438	positive		94.688
125439	negative		96.9

Colony morphology

@ref	Colony color	Incubation period	Medium used
18370	Sand yellow (1002)	10-14 days	ISP 2
18370	Zinc yellow (1018)	10-14 days	ISP 3
18370	Ivory (1014)	10-14 days	ISP 4
18370	Colorless	10-14 days	ISP 5
18370	Honey yellow (1005)	10-14 days	ISP 6
18370	Ivory (1014)	10-14 days	ISP 7

Multicellular morphology

@ref	Forms multicellular complex	Medium name
18370		ISP 2
18370		ISP 3
18370		ISP 4
18370		ISP 5
18370		ISP 6
18370		ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
4824	OXOID NUTRIENT BROTH (DSMZ Medium 948)	Medium recipe at MediaDive	Name: OXOID NUTRIENT BROTH (DSMZ Medium 948) Composition: Nutrient broth 13.0 g/l Distilled water
18370	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18370	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
18370	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18370	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18370	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18370	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
37402	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
121215	CIP Medium 72	Medium recipe at CIP
121215	CIP Medium 3	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
4824	positive	growth	28
18370	positive	optimum	28	mesophilic
37402	positive	growth	30	mesophilic
67770	positive	growth	28	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
121215	obligate aerobe
125438	aerobe	90.494

Spore formation

@ref	Spore formation	Confidence
125439		98.8

Compound production

4824

CompoundL glutamic acid

Halophily

@ref	Salt	Growth	Tested relation	Concentration
18370	NaCl	positive	maximum	7.5 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
18370	22599 ChEBI	arabinose	-
68368	29016 ChEBI	arginine	-	hydrolysis	from API 20E
18370	62968 ChEBI	cellulose	-
68368	16947 ChEBI	citrate	-	assimilation	from API 20E
68379	17634 ChEBI	D-glucose	-	fermentation	from API Coryne
68379	16899 ChEBI	D-mannitol	+	fermentation	from API Coryne
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68379	4853 ChEBI	esculin	-	hydrolysis	from API Coryne
18370	28757 ChEBI	fructose	-
68379	5291 ChEBI	gelatin	+	hydrolysis	from API Coryne
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
18370	17234 ChEBI	glucose	+
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68368	25094 ChEBI	lysine	-	degradation	from API 20E
68379	17306 ChEBI	maltose	+	fermentation	from API Coryne
18370	29864 ChEBI	mannitol	-
18370	17268 ChEBI	myo-inositol	+
68379	17632 ChEBI	nitrate	+	reduction	from API Coryne
121215	17632 ChEBI	nitrate	-	reduction
121215	16301 ChEBI	nitrite	-	reduction
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
18370	16634 ChEBI	raffinose	+
18370	26546 ChEBI	rhamnose	-
18370	17992 ChEBI	sucrose	+
68379	17992 ChEBI	sucrose	+	fermentation	from API Coryne
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68379	16199 ChEBI	urea	+	hydrolysis	from API Coryne
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
121215	35581 ChEBI	indole
68368	35581 ChEBI	indole	from API 20E
67770	29985 ChEBI	L-glutamate

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	+		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68379	alkaline phosphatase	-	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
68382	beta-galactosidase	-	3.2.1.23	from API zym
68379	beta-galactosidase	+	3.2.1.23	from API Coryne
68368	beta-galactosidase	-	3.2.1.23	from API 20E
68379	beta-glucosidase	-	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	+	3.2.1.31	from API Coryne
121215	catalase	+	1.11.1.6
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68379	gelatinase	+		from API Coryne
68368	gelatinase	+		from API 20E
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68379	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API Coryne
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
121215	oxidase	-
68379	pyrazinamidase	+	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	+	3.4.19.3	from API Coryne
68382	trypsin	+	3.4.21.4	from API zym
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
121215	urease	-	3.5.1.5
68379	urease	+	3.5.1.5	from API Coryne
68368	urease	-	3.5.1.5	from API 20E
68382	valine arylamidase	+		from API zym

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
18370	-	-	-	-	-	-	-	-	-	+	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
18370	+	+	+	-	+	+	+	+	-	+	+	not determinedn.d.	-	-	-	+	+	-	+	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
18370	not determinedn.d.	+	+	+	-	+	+	+	+	-	+	+	-	-	-	+	+	-	+	-
121215	-	+	+	+	-	+	+	-	+	-	-	+	-	-	-	+	-	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Waste	#Wastewater

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
4824	sewage	Mysore	India	IND	Asia
67770	Sewage	Mysore City	India	IND	Asia
121215	Environment, Sewage

Taxonmaps

69479

Global distribution of 16S sequence AJ639831 (>99% sequence identity) for Glutamicibacter from Microbeatlas

Aquatic Samples	29
Soil Samples	26
Animal Samples	63
Plant Samples	9
Total Samples	127

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
4824	1	Risk group (German classification) According to TRBA 466
18370	1
121215	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM279777v1 assembly for Glutamicibacter mysorens DSM 12798	contig	GCA_002797775	257984.4	2627854287	257984	79.09
67770	ASM155211v1 assembly for Glutamicibacter mysorens NBRC 103060	contig	GCA_001552115	1216975.4	2681812973	1216975	75.33

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Arthrobacter mysorens partial 16S rRNA gene, type strain DSM 12798T	AJ617482	1491	257984
20218	Arthrobacter mysorens partial 16S rRNA gene, type strain LMG 16219T	AJ639831	1478	257984
124043	Glutamicibacter mysorens strain LMG 16219 16S ribosomal RNA gene, partial sequence.	MN865826	1379	257984

GC content

@ref	GC-content (mol%)	Method
67770	62	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Glutamicibacter mysorens NBRC 103060
NCBI: GCA_001552115

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	aerobe	81.30	no
125439	gram_stain	BacteriaNetⓘ	negative	96.90	no
125439	motility	BacteriaNetⓘ	yes	74.20	no
125439	spore_formation	BacteriaNetⓘ	no	98.80	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Glutamicibacter mysorens NBRC 103060
PATRIC: 1216975.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	94.69	no
125438	anaerobic	anaerobicⓘ	no	98.27	yes
125438	spore-forming	spore-formingⓘ	no	72.25	no
125438	aerobic	aerobicⓘ	yes	90.49	yes
125438	thermophilic	thermophileⓘ	no	98.46	yes
125438	flagellated	motile2+ⓘ	no	92.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Airborne bacteria in show caves from Southern Spain.	Dominguez-Monino I, Jurado V, Rogerio-Candelera MA, Hermosin B, Saiz-Jimenez C.	Microb Cell	10.15698/mic2021.10.762	2021
	Biogenic Silver Nanoparticles Produced by Soil Rare Actinomycetes and Their Significant Effect on Aspergillus-derived mycotoxins.	Abd El-Ghany MN, Hamdi SA, Korany SM, Elbaz RM, Emam AN, Farahat MG.	Microorganisms	10.3390/microorganisms11041006	2023
Phylogeny	Phylogenomic Analysis Reveals That Arthrobacter mysorens Nand and Rao 1972 (Approved Lists 1980) and Glutamicibacter mysorens Busse 2016 are Later Heterotypic Synonyms of Arthrobacter nicotianae Giovannozzi-Sermanni 1959 (Approved Lists 1980) and Glutamicibacter nicotianae Busse 2016.	Deb S, Das L, Das SK	Curr Microbiol	10.1007/s00284-020-02176-z	2020
Phylogeny	Glutamicibacter halophytocola sp. nov., an endophytic actinomycete isolated from the roots of a coastal halophyte, Limonium sinense.	Feng WW, Wang TT, Bai JL, Ding P, Xing K, Jiang JH, Peng X, Qin S	Int J Syst Evol Microbiol	10.1099/ijsem.0.001775	2017

References

#4824	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 12798
#18370	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#37402	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#121215	Collection of Institut Pasteur ; Curators of the CIP; CIP 102716
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Glutamicibacter nicotianae (DSM 12798, ATCC 33408, NCIMB 10583)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

API coryne

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_002797775

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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