Strain identifier

BacDive ID: 7529

Type strain: Yes

Species: Pseudarthrobacter oxydans

Strain Designation: Xanthum

Strain history: CIP <- 2001, CCUG <- 1985, DSMZ <- ATCC <- R.J. Reynolds Tobacco Co: strain Xanthum

NCBI tax ID(s): 1671 (species)

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General

@ref: 8544

BacDive-ID: 7529

DSM-Number: 20119

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, oval-shaped

description: Pseudarthrobacter oxydans Xanthum is an aerobe, mesophilic, Gram-positive bacterium that was isolated from air.

NCBI tax id

  • NCBI tax id: 1671
  • Matching level: species

strain history

@refhistory
8544<- ATCC <- R.J. Reynolds Tobacco Co., strain Xanthum
67770ATCC 14358 <-- R. J. Reynolds Tobacco Co.; strain Xanthum.
121482CIP <- 2001, CCUG <- 1985, DSMZ <- ATCC <- R.J. Reynolds Tobacco Co: strain Xanthum

doi: 10.13145/bacdive7529.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Micrococcaceae
  • genus: Pseudarthrobacter
  • species: Pseudarthrobacter oxydans
  • full scientific name: Pseudarthrobacter oxydans (Sguros 1954) Busse 2016

  • synonyms

    • @ref: 20215
    • synonym: Arthrobacter oxydans

@ref: 8544

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micrococcaceae

genus: Pseudarthrobacter

species: Pseudarthrobacter oxydans

full scientific name: Pseudarthrobacter oxydans (Sguros 1954) Busse 2016

strain designation: Xanthum

type strain: yes

Morphology

cell morphology

  • @ref: 121482
  • gram stain: positive
  • cell shape: oval-shaped
  • motility: no

colony morphology

@refcolony colorincubation periodmedium used
18622Ivory (1014)10-14 daysISP 2
18622Light ivory (1015)10-14 daysISP 3
121482

multicellular morphology

@refforms multicellular complexmedium name
18622noISP 2
18622noISP 3

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
18622ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18622ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
33034MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
8544CORYNEBACTERIUM AGAR (DSMZ Medium 53)yesName: CORYNEBACTERIUM AGAR (DSMZ Medium 53) Composition: Agar 15.0 g/l Casein peptone 10.0 g/l NaCl 5.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/53
121482CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
18622positiveoptimum28mesophilic
8544positivegrowth30mesophilic
33034positivegrowth30mesophilic
46682positivegrowth30-37mesophilic
67770positivegrowth30mesophilic
121482positivegrowth10-41
121482nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
46682aerobe
121482obligate aerobe

compound production

@refcompound
8544nicotinblue I
8544nicotinblue II

halophily

@refsaltgrowthtested relationconcentration
18622NaClpositivegrowth0 %
121482NaClpositivegrowth0-6 %
121482NaClnogrowth8 %
121482NaClnogrowth10 %

murein

  • @ref: 8544
  • murein short key: A11.23
  • type: A3alpha L-Lys-L-Ser-L-Thr-L-Ala

observation

  • @ref: 67770
  • observation: quinones: MK-9(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1862262968cellulose+
1862216634raffinose+
1862226546rhamnose+
1862228757fructose+
1862229864mannitol+
1862217268myo-inositol+
1862218222xylose+
1862217992sucrose+
1862222599arabinose+
1862217234glucose+
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol+builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose+builds acid from
683716731melezitose+builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose+builds acid from
6837128053melibiose+builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol+builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol+builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose+builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose+builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose+builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol+builds acid from
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis
12148216947citrate-carbon source
1214824853esculin+hydrolysis
121482606565hippurate+hydrolysis
12148217632nitrate+reduction
12148216301nitrite-reduction
12148217632nitrate-respiration
683794853esculin-hydrolysis
6837916199urea+hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose+fermentation
6837916988D-ribose+fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol+fermentation
6837917992sucrose+fermentation
6837928087glycogen-fermentation

antibiotic resistance

  • @ref: 121482
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno
12148235581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin-
6836835581indole-
12148215688acetoin+
12148217234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382alkaline phosphatase+3.1.3.1
68379catalase+1.11.1.6
68379gelatinase+
68379urease+3.5.1.5
68379beta-glucosidase-3.2.1.21
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase-3.5.1.B15
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23
121482oxidase-
121482beta-galactosidase+3.2.1.23
121482alcohol dehydrogenase-1.1.1.1
121482gelatinase+
121482amylase+
121482DNase-
121482caseinase+3.4.21.50
121482catalase+1.11.1.6
121482tween esterase-
121482gamma-glutamyltransferase+2.3.2.2
121482lecithinase-
121482lipase-
121482lysine decarboxylase-4.1.1.18
121482ornithine decarboxylase-4.1.1.17
121482phenylalanine ammonia-lyase-4.3.1.24
121482tryptophan deaminase-
121482urease-3.5.1.5

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYGControlCAT
18622--+-++++-++++-+--+-
46682+-++-++--+++-++++--+

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18622+-+-++++-++++-+----
121482++--+--+-++++-+-----

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18622+-----+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
121482+--+++---++++-+-+++--+--+/-----++--++--+-+/----------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121482+++++-++++++++-++++-+++--+-++--++++++--------+---+++-+--+--++++++++----+++-+---++-----+-++++-++-+--

Isolation, sampling and environmental information

isolation

@refsample typeisolation date
8544air
46682Air
67770Air
121482Environment, Air1985

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Air

taxonmaps

  • @ref: 69479
  • File name: preview.99_130.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_99;97_106;98_115;99_130&stattab=map
  • Last taxonomy: Pseudarthrobacter
  • 16S sequence: X83408
  • Sequence Identity:
  • Total samples: 1526
  • soil counts: 822
  • aquatic counts: 109
  • animal counts: 156
  • plant counts: 439

Safety information

risk assessment

@refbiosafety levelbiosafety level commentpathogenicity human
186221Hazard group
85441Risk group (German classification)yes, in single cases
1214821Risk group (French classification)

Sequence information

16S sequences

  • @ref: 8544
  • description: A.oxydans 16S rRNA gene
  • accession: X83408
  • length: 1486
  • database: ena
  • NCBI tax ID: 1671

GC content

@refGC-contentmethod
854462.7
6777065.9Buoyant density centrifugation (BD)
6777062.7thermal denaturation, midpoint method (Tm)
6777063.1high performance liquid chromatography (HPLC)

External links

@ref: 8544

culture collection no.: DSM 20119, ATCC 14358, NCIB 9333, CCUG 17757, CIP 107005, JCM 2521, BCRC 11573, CECT 386, CGMCC 1.1925, HAMBI 1857, IAM 14589, IFO 12138, IMET 10684, KCTC 3383, LMG 3816, MTCC 3699, NBRC 12138, NCIMB 9333, NRIC 0154, VKM Ac-1114

straininfo link

  • @ref: 76974
  • straininfo: 13578

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12054218Arthrobacter roseus sp. nov., a psychrophilic bacterium isolated from an antarctic cyanobacterial mat sample.Reddy GSN, Prakash JSS, Matsumoto GI, Stackebrandt E, Shivaji SInt J Syst Evol Microbiol10.1099/00207713-52-3-10172002Antarctic Regions, Arthrobacter/*chemistry/*classification/genetics, Bacterial Typing Techniques, Biomass, DNA, Ribosomal/analysis, Fresh Water/*microbiology, Molecular Sequence Data, Peptidoglycan/analysis, Phenotype, Phylogeny, Pigments, Biological/metabolism, RNA, Ribosomal, 16S/*genetics, Sequence Analysis, DNA, *TemperatureGenetics
Metabolism18437357Genetic analysis of phenylacetic acid catabolism in Arthrobacter oxydans CECT386.Navarro-Llorens JM, Drzyzga O, Perera JArch Microbiol10.1007/s00203-008-0370-x2008Arthrobacter/*genetics/*metabolism, Chromosomes, Bacterial/genetics, Cloning, Molecular, DNA, Bacterial/genetics, Escherichia coli/genetics, *Genes, Bacterial, Genetic Complementation Test, Molecular Sequence Data, Multigene Family, Open Reading Frames, Operon, Phenylacetates/*metabolism, Plasmids, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNAEnzymology
Phylogeny18676480Arthrobacter defluvii sp. nov., 4-chlorophenol-degrading bacteria isolated from sewage.Kim KK, Lee KC, Oh HM, Kim MJ, Eom MK, Lee JSInt J Syst Evol Microbiol10.1099/ijs.0.65550-02008Arthrobacter/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Chlorophenols/*metabolism, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Korea, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Species SpecificityGenetics
Phylogeny25150449Arthrobacter ginsengisoli sp. nov., isolated from soil of a ginseng field.Siddiqi MZ, Kim YJ, Hoang VA, Siddiqi MH, Huq MA, Yang DCArch Microbiol10.1007/s00203-014-1025-82014Arthrobacter/classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Genes, rRNA, Lipids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, *Panax, Peptidoglycan/chemistry, Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil MicrobiologyGenetics
Phylogeny33048040Pseudarthrobacter psychrotolerans sp. nov., a cold-adapted bacterium isolated from Antarctic soil.Shin Y, Lee BH, Lee KE, Park WInt J Syst Evol Microbiol10.1099/ijsem.0.0045052020Antarctic Regions, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Glycolipids/chemistry, Micrococcaceae/*classification/isolation & purification, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivativesTranscriptome

Reference

@idauthorscataloguedoi/urltitle
8544Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20119)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20119
18622Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM20119.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
33034Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19197
46682Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 17757)https://www.ccug.se/strain?id=17757
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76974Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13578.1StrainInfo: A central database for resolving microbial strain identifiers
121482Curators of the CIPCollection of Institut Pasteur (CIP 107005)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107005