Strain identifier

BacDive ID: 7494

Type strain: Yes

Species: Rhodoglobus aureus

Strain history: CIP <- 2003, S. Shivaji, CCMB, Hyderabad, India: strain CMS 81y

NCBI tax ID(s): 191497 (species)

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General

@ref: 5836

BacDive-ID: 7494

DSM-Number: 15303

keywords: 16S sequence, Bacteria, obligate aerobe, psychrophilic, Gram-positive, rod-shaped

description: Rhodoglobus aureus DSM 15303 is an obligate aerobe, psychrophilic, Gram-positive bacterium that was isolated from cyanobacterial mat sample.

NCBI tax id

  • NCBI tax id: 191497
  • Matching level: species

strain history

@refhistory
5836<- S. Shivaji; CMS 81y
399422003, S. Shivaji, CCMB, Hyderabad, India: strain CMS 81y
67770MTCC 4657 <-- G. S. N. Reddy CMS 81y.
116571CIP <- 2003, S. Shivaji, CCMB, Hyderabad, India: strain CMS 81y

doi: 10.13145/bacdive7494.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Microbacteriaceae
  • genus: Rhodoglobus
  • species: Rhodoglobus aureus
  • full scientific name: Rhodoglobus aureus (Reddy et al. 2003) An et al. 2011

  • synonyms

    • @ref: 20215
    • synonym: Leifsonia aurea

@ref: 5836

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Microbacteriaceae

genus: Rhodoglobus

species: Rhodoglobus aureus

full scientific name: Rhodoglobus aureus (Reddy et al. 2003) An et al. 2011

type strain: yes

Morphology

cell morphology

  • @ref: 116571
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refcolony colorincubation periodmedium used
18391Beige (1001)10-14 daysISP 2
18391Beige (1001)10-14 daysISP 3
18391Beige (1001)10-14 daysISP 4
18391Colorless10-14 daysISP 5
18391Beige (1001)10-14 daysISP 6
18391Beige (1001)10-14 daysISP 7
116571

multicellular morphology

@refforms multicellular complexmedium name
18391noISP 2
18391noISP 3
18391noISP 4
18391noISP 5
18391noISP 6
18391noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5836R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
18391ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18391ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18391ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18391ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18391ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18391ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
39942MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
116571CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
18391positiveoptimum22psychrophilic
5836positivegrowth20psychrophilic
39942positivegrowth22psychrophilic
67770positivegrowth22psychrophilic
116571positivegrowth10-25psychrophilic
116571nogrowth30mesophilic
116571nogrowth37mesophilic
116571nogrowth41thermophilic
116571nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 116571
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
116571NaClpositivegrowth0-6 %
116571NaClnogrowth8 %
116571NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-11

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1839117234glucose-
1839122599arabinose+
1839117992sucrose+
1839118222xylose-
1839117268myo-inositol+
1839129864mannitol+
1839128757fructose+
1839126546rhamnose+
1839116634raffinose-
1839162968cellulose+
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin+builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837115824D-fructose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
1165714853esculin-hydrolysis
116571606565hippurate+hydrolysis
11657117632nitrate+reduction
11657116301nitrite-reduction
11657117632nitrate+respiration
683794853esculin+hydrolysis
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837917992sucrose-fermentation

antibiotic resistance

  • @ref: 116571
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 116571
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11657115688acetoin+
11657117234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-chymotrypsin-3.4.21.1
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
116571oxidase-
116571beta-galactosidase+3.2.1.23
116571alcohol dehydrogenase-1.1.1.1
116571gelatinase-
116571amylase-
116571DNase-
116571caseinase-3.4.21.50
116571catalase+1.11.1.6
116571tween esterase-
116571gamma-glutamyltransferase-2.3.2.2
116571lecithinase-
116571lipase-
116571lysine decarboxylase-4.1.1.18
116571ornithine decarboxylase-4.1.1.17
116571phenylalanine ammonia-lyase-4.3.1.24
116571tryptophan deaminase-
116571urease-3.5.1.5

API coryne

@refPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELSAC
18391----++-+---

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18391+++-++-----++-++-+-
116571-++-++-+-+----+-----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116571----+/-----+/-+/-++/-----+/----------+/-----+-------------+/---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116571+++-+------------------+-------+-+--------------------------+--------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcontinentcountryorigin.countryisolation date
5836cyanobacterial mat sampleMcMurdo, Wright ValleyAustralia and Oceania
67770Cyanobacterial mat from a pondWright Valley, McMurdoAntarcticaAntarcticaATA
116571Cyanobacterial material samplesMcMurdoAntarcticaAntarcticaATA1995

isolation source categories

Cat1Cat2Cat3
#Environmental#Microbial community#Microbial mat
#Host#Microbial#Bacteria

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
58361Risk group (German classification)
183911
1165711Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5836
  • description: Leifsonia aureus 16S rRNA gene, type strain CMS 81
  • accession: AJ438586
  • length: 1513
  • database: ena
  • NCBI tax ID: 191497

GC content

@refGC-contentmethod
583664
6777064thermal denaturation, midpoint method (Tm)

External links

@ref: 5836

culture collection no.: DSM 15303, CIP 107785, IAM 15170, JCM 12762, MTCC 4657, CMS 81y, NBRC 104579

straininfo link

  • @ref: 76940
  • straininfo: 113589

literature

  • topic: Phylogeny
  • Pubmed-ID: 12892114
  • title: Leifsonia rubra sp. nov. and Leifsonia aurea sp. nov., psychrophiles from a pond in Antarctica.
  • authors: Reddy GSN, Prakash JSS, Srinivas R, Matsumoto GI, Shivaji S
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.02396-0
  • year: 2003
  • mesh: Actinomycetales/classification/genetics/*isolation & purification/*metabolism, Antarctic Regions, Base Composition, DNA, Bacterial/chemistry/genetics, Fatty Acids/metabolism, Fresh Water/microbiology, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Terminology as Topic
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitle
5836Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15303)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15303
18391Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM15303.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
39942Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5258
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
76940Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID113589.1StrainInfo: A central database for resolving microbial strain identifiers
116571Curators of the CIPCollection of Institut Pasteur (CIP 107785)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107785