Archive BacDiveID:10.13145/bacdive7437.20230509.8.1

Strain identifier

BacDive ID: 7437

Type strain:

Species: Microbacterium lacus

Strain Designation: A5E-52

Strain history: DSM 18910 <-- H. Kasai <-- K. Adachi A5E-52.

NCBI tax ID(s): 415217 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 7770

BacDive-ID: 7437

DSM-Number: 18910

keywords: 16S sequence, Bacteria, mesophilic

description: Microbacterium lacus A5E-52 is a mesophilic bacterium that was isolated from sediment.

NCBI tax id

NCBI tax id: 415217
Matching level: species

strain history

@ref	history
7770	<- H. Kasi <- K. Adachi; A5E-52
67770	DSM 18910 <-- H. Kasai <-- K. Adachi A5E-52.

doi: 10.13145/bacdive7437.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/actinomycetota
domain: Bacteria
phylum: Actinomycetota
class: Actinomycetes
order: Micrococcales
family: Microbacteriaceae
genus: Microbacterium
species: Microbacterium lacus
full scientific name: Microbacterium lacus Kageyama et al. 2008

@ref: 7770

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Microbacteriaceae

genus: Microbacterium

species: Microbacterium lacus

full scientific name: Microbacterium lacus Kageyama et al. 2008

strain designation: A5E-52

type strain: yes

Culture and growth conditions

culture medium

@ref: 7770
name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)
growth: yes
link: https://mediadive.dsmz.de/medium/514
composition: Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water

culture temp

@ref	growth	type	temperature	range
7770	positive	growth	28	mesophilic
67770	positive	growth	28	mesophilic

Physiology and metabolism

observation

@ref: 67770
observation: quinones: MK-12, MK-13

Isolation, sampling and environmental information

isolation

@ref	sample type	geographic location	country	origin.country	continent
7770	sediment	Shinji lake, Shimane pref.	Japan	JPN	Asia
67770	Sediment at the mouth of the Hiratafuna River to Lake Shinjiko	Shimane Pref.	Japan	JPN	Asia

isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Sediment
#Environmental	#Terrestrial	#Sediment

Safety information

risk assessment

@ref: 7770
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref: 7770
description: Microbacterium lacus gene for 16S rRNA, partial sequence
accession: AB286030
length: 1463
database: ena
NCBI tax ID: 415217

GC content

@ref	GC-content	method
7770	69
67770	69	high performance liquid chromatography (HPLC)

External links

@ref: 7770

culture collection no.: DSM 18910, JCM 15575, MBIC 08279, KCTC 19672, NBRC 103566

straininfo link

@ref: 76885
straininfo: 404307

literature

topic	Pubmed-ID	title	authors	journal	DOI	year	mesh	topic2
Phylogeny	19567581	Microbacterium invictum sp. nov., isolated from homemade compost.	Vaz-Moreira I, Lopes AR, Faria C, Sproer C, Schumann P, Nunes OC, Manaia CM	Int J Syst Evol Microbiol	10.1099/ijs.0.005561-0	2009	Actinomycetales/classification/genetics/isolation & purification/physiology, Aerobiosis, Base Composition, Catalase/metabolism, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Hydrogen-Ion Concentration, Locomotion, Molecular Sequence Data, Nucleic Acid Hybridization, Oxidoreductases/metabolism, Peptidoglycan/chemistry, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, *Soil Microbiology, Temperature, Vitamin K 2/analysis	Metabolism
Phylogeny	26296337	Microbacterium rhizomatis sp. nov., a beta-glucosidase-producing bacterium isolated from rhizome of Korean mountain ginseng.	Hoang VA, Kim YJ, Nguyen NL, Kang CH, Kang JP, Singh P, Farh ME, Yang DU, Yang DC	Int J Syst Evol Microbiol	10.1099/ijsem.0.000399	2015	*Actinomycetales/classification, Bacterial Typing Techniques, Base Composition, Cellulases/genetics, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genotype, Glucosidases, Glycolipids/chemistry, Lipids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization/genetics, Panax/microbiology, Peptidoglycan/chemistry, Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Rhizome, Sequence Analysis, DNA, Soil Microbiology	Genetics
Phylogeny	27498827	Microbacterium diaminobutyricum sp. nov., isolated from Halimione portulacoides, which contains diaminobutyric acid in its cell wall, and emended description of the genus Microbacterium.	Fidalgo C, Riesco R, Henriques I, Trujillo ME, Alves A	Int J Syst Evol Microbiol	10.1099/ijsem.0.001379	2016	Actinomycetales/classification/genetics/isolation & purification, Amaranthaceae/microbiology, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Fatty Acids/chemistry, Multilocus Sequence Typing, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, Portugal, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA	Transcriptome

Reference

@id	authors	catalogue	doi/url	title
7770	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18910)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-18910
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
67770	Curators of the JCM		https://jcm.brc.riken.jp/en/
76885	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID404307.1	StrainInfo: A central database for resolving microbial strain identifiers