Strain identifier

BacDive ID: 7391

Type strain: Yes

Species: Leucobacter weissii

Strain Designation: S27

Strain history: CCM 8762 <-- R. Pukall; DSMZ, Germany <-- R. Hensel; Bot. Inst. of Ludwig-Maximilians-Univ. München, Germany; S27.

NCBI tax ID(s): 1983706 (species)

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General

@ref: 8969

BacDive-ID: 7391

DSM-Number: 20621

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-positive, rod-shaped, colony-forming

description: Leucobacter weissii S27 is a mesophilic, Gram-positive, rod-shaped bacterium that forms circular colonies and was isolated from activated sludge.

NCBI tax id

  • NCBI tax id: 1983706
  • Matching level: species

strain history

@refhistory
8969<- R. Hensel, S27
67770CCM 8762 <-- R. Pukall; DSMZ, Germany <-- R. Hensel; Bot. Inst. of Ludwig-Maximilians-Univ. München, Germany; S27.

doi: 10.13145/bacdive7391.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Microbacteriaceae
  • genus: Leucobacter
  • species: Leucobacter weissii
  • full scientific name: Leucobacter weissii Schumann and Pukall 2017

@ref: 8969

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Microbacteriaceae

genus: Leucobacter

species: Leucobacter weissii

full scientific name: Leucobacter weissii Schumann and Pukall 2017

strain designation: S27

type strain: yes

Morphology

cell morphology

  • @ref: 65225
  • gram stain: positive
  • cell length: 2 µm
  • cell width: 0.5 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 65225
  • colony size: 1-3 mm
  • colony color: yellow
  • colony shape: circular
  • incubation period: 3 days
  • medium used: enriched nutrient agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8969TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
65225enriched nutrient agaryes

culture temp

@refgrowthtypetemperaturerange
8969positivegrowth30mesophilic
65225positiveoptimum28-37mesophilic
65225nogrowth10-15psychrophilic
65225nogrowth45thermophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepH
65225positiveoptimum7
65225nogrowth5

Physiology and metabolism

spore formation

  • @ref: 65225
  • spore formation: no

halophily

  • @ref: 65225
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0.5-4 %

murein

  • @ref: 8969
  • murein short key: B11
  • type: B2delta {Gly} [L-Ala] D-Glu-D-Dab-L-Thr

observation

  • @ref: 67770
  • observation: quinones: MK-11

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6522515588D-malate-assimilation
6522516651(S)-lactate-assimilation
6522515589L-malate-assimilation
65225172562-deoxyadenosine-assimilation
65225309162-oxoglutarate-assimilation
65225286442-oxopentanoate-assimilation
65225620642,3-butanediol-assimilation
65225739183-O-methyl-D-glucose-assimilation
65225181014-hydroxyphenylacetic acid-assimilation
6522530089acetate+assimilation
6522513705acetoacetate+assimilation
6522516335adenosine-assimilation
6522540585alpha-cyclodextrin-assimilation
6522517925alpha-D-glucose-assimilation
65225645522-hydroxybutyrate-assimilation
6522516027amp-assimilation
6522527613amygdalin-assimilation
6522518305arbutin-assimilation
65225495055beta-cyclodextrin-assimilation
652258295beta-hydroxybutyrate-assimilation
6522573706bromosuccinate-assimilation
6522517057cellobiose-assimilation
6522516947citrate-assimilation
6522523456cyclodextrin-assimilation
6522515570D-alanine-assimilation
6522518333D-arabitol-assimilation
6522529990D-aspartate-assimilation
6522515824D-fructose-assimilation
6522578697D-fructose 6-phosphate-assimilation
6522528847D-fucose-assimilation
6522512936D-galactose-assimilation
6522518024D-galacturonic acid-assimilation
652258391D-gluconate-assimilation
6522514314D-glucose 6-phosphate-assimilation
6522515748D-glucuronate-assimilation
6522516899D-mannitol-assimilation
6522516024D-mannose-assimilation
6522527605D-psicose-assimilation
6522516988D-ribose-assimilation
6522533801D-saccharate-assimilation
6522516523D-serine-assimilation
6522517924D-sorbitol-assimilation
6522516443D-tagatose-assimilation
6522565327D-xylose-assimilation
6522515740formate-assimilation
6522516537galactarate-assimilation
6522516865gamma-aminobutyric acid+assimilation
65225167244-hydroxybutyrate-assimilation
6522528066gentiobiose-assimilation
6522529042glucose 1-phosphate-assimilation
6522532323glucuronamide-assimilation
6522517754glycerol-assimilation
6522514336glycerol 1-phosphate-assimilation
6522528087glycogen-assimilation
6522517596inosine-assimilation
6522515443inulin-assimilation
6522521217L-alaninamide+assimilation
6522516977L-alanine+assimilation
6522573786L-alanylglycine-assimilation
6522530849L-arabinose-assimilation
6522516467L-arginine+assimilation
6522517196L-asparagine-assimilation
6522518287L-fucose-assimilation
6522517464L-galactonic acid gamma-lactone-assimilation
6522529985L-glutamate-assimilation
6522515971L-histidine+assimilation
6522518183L-pyroglutamic acid-assimilation
6522562345L-rhamnose-assimilation
6522517115L-serine-assimilation
6522575144lactamide-assimilation
6522517716lactose-assimilation
652256359lactulose-assimilation
6522517306maltose-assimilation
6522561993maltotriose-assimilation
6522528808mannan-assimilation
652256731melezitose-assimilation
6522528053melibiose-assimilation
6522574611methyl (R)-lactate-assimilation
6522555507methyl alpha-D-galactoside-assimilation
65225320061methyl alpha-D-glucopyranoside-assimilation
6522543943methyl alpha-D-mannoside-assimilation
6522517540methyl beta-D-galactoside-assimilation
65225320055methyl beta-D-glucopyranoside-assimilation
6522551850methyl pyruvate+assimilation
6522575146monomethyl succinate-assimilation
6522517268myo-inositol-assimilation
6522563153N-acetyl-D-mannosamine-assimilation
6522544337N-acetyl-L-glutamate-assimilation
65225506227N-acetylglucosamine-assimilation
6522573784glycyl-l-glutamate-assimilation
6522518394palatinose-assimilation
6522517309pectin-assimilation
6522517272propionate-assimilation
6522517148putrescine+assimilation
6522515361pyruvate+assimilation
6522526490quinate-assimilation
6522516634raffinose-assimilation
6522517814salicin-assimilation
6522532488sedoheptulosan-assimilation
6522517164stachyose-assimilation
65225143136succinamate-assimilation
6522530031succinate-assimilation
6522517992sucrose-assimilation
6522517748thymidine-assimilation
6522563528thymidine 5'-monophosphate-assimilation
6522527082trehalose-assimilation
6522532528turanose-assimilation
6522553423tween 40+assimilation
6522553426tween 80-assimilation
6522516704uridine-assimilation
6522516695uridine 5'-monophosphate-assimilation
6522517151xylitol-assimilation

enzymes

@refvalueactivityec
65225catalase+1.11.1.6
65225cytochrome oxidase-1.9.3.1

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    65225C14:0 iso0.5
    65225C15:0 iso25.1
    65225C15:0 anteiso30.3
    65225C16:0 iso20.7
    65225C16:02.5
    65225C17:0 iso6.3
    65225C17:0 anteiso14.1
  • type of FA analysis: whole cell analysis
  • incubation medium: trypticase soy yeast extract agar
  • agar/liquid: agar
  • incubation temperature: 28
  • software version: Sherlock 6.1
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling dategeographic locationenrichment cultureenrichment culture compositionenrichment culture temperatureisolation procedure
8969activated sludgeGermanyDEUEurope
65225aerated laboratory scale fermenter (temperature: 15-17°C; volume: 20 l; retention time: 10 days) fed with wastes of a yeast factoryGermanyDEUEurope1982Botanical Institute of the University Munichenriched nutrient agar mediumwith 1.0 g glucose, 7.5 g peptone (Merck), 5.0 g meat extract Lab Lemco' (Oxoid), 2.5 g yeast extract (Oxoid), 2.5 g casamino acids (Difco), 5.0 g NaCl, 13 g agar (Merck) in 1 l tap water (pH 7.2-7.4)20the plates were incubated until single colonies could be selected for further purification
67770Aerated laboratory scale fermenter fed with wastes of a yeast factory at the Botanical Institute of the University MunichGermanyDEUEurope

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Waste
  • Cat3: #Activated sludge

Safety information

risk assessment

  • @ref: 8969
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 8969
  • description: Leucobacter sp. DSM 20621 partial 16S rRNA gene, strain DSM 20621
  • accession: LT844632
  • length: 1515
  • database: ena
  • NCBI tax ID: 1983706

Genome sequences

  • @ref: 66792
  • description: Leucobacter weissii S27
  • accession: GCA_017565745
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 1983706

GC content

  • @ref: 8969
  • GC-content: 70.1
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno96.524yes
gram-positiveyes89.109no
anaerobicno98.786no
aerobicyes87.663no
halophileno82.985no
spore-formingno94.4yes
thermophileno99.578no
glucose-utilyes63.602yes
motileno89.843yes
glucose-fermentno89.103no

External links

@ref: 8969

culture collection no.: DSM 20621, CCM 8762, JCM 32600

straininfo link

  • @ref: 76840
  • straininfo: 49660

literature

  • topic: Phylogeny
  • Pubmed-ID: 29087268
  • title: Leucobacter weissii sp. nov., an isolate from activated sludge once described as first representative of the peptidoglycan variation B2delta, and emended description of the genus Leucobacter.
  • authors: Schumann P, Pukall R
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002454
  • year: 2017
  • mesh: Actinobacteria/*classification/genetics/isolation & purification, Aminobutyrates/chemistry, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Fatty Acids/chemistry, Germany, Glycolipids/chemistry, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Vitamin K 2/chemistry
  • topic2: Transcriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8969Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20621)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20621
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
65225Peter Schumann, Rüdiger Pukall10.1099/ijsem.0.002454Leucobacter weissii sp. nov., an isolate from activated sludge once described as first representative of the peptidoglycan variation B2d, and emended description of the genus LeucobacterIJSEM 67: 5244-5251 201729087268
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
76840Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49660.1StrainInfo: A central database for resolving microbial strain identifiers