Name: Leucobacter weissii S27
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7391

Type strain:

Species: Leucobacter weissii

Strain Designation: S27

Culture col. no.: DSM 20621, CCM 8762, JCM 32600

Strain history: CCM 8762 <-- R. Pukall; DSMZ, Germany <-- R. Hensel; Bot. Inst. of Ludwig-Maximilians-Univ. München, Germany; S27.

NCBI tax ID(s): 1983706 (species)

SI-ID 49660

DSM 20621

Section

Leucobacter weissii S27 is a mesophilic, Gram-positive, rod-shaped bacterium that forms circular colonies and was isolated from activated sludge.

colony-forming
Gram-positive
rod-shaped
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Microbacteriaceae*
	Genus	*Leucobacter*
	Species	**Leucobacter weissii**
	Full Scientific Name (LPSN)	Leucobacter weissii Schumann and Pukall 2017

Information on morphological and physiological properties Morphology

[Ref.: #65225]	Gram stain	positive

[Ref.: #65225]	Cell length	2 µm

[Ref.: #65225]	Cell width	0.5 µm

[Ref.: #65225]	Cell shape	rod-shaped

[Ref.: #65225]	Motility	no

[Ref.: #65225]	Colony size	1-3 mm
[Ref.: #65225]	Colony color	yellow
[Ref.: #65225]	Colony shape	circular
[Ref.: #65225]	Incubation period	3 days
[Ref.: #65225]	Cultivation medium used	enriched nutrient agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8969]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #8969]

Culture medium growth

[Ref.: #8969]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8969]

Culture medium composition

expand / minimize

[Ref.: #65225]

Culture medium

enriched nutrient agar

[Ref.: #65225]

Culture medium growth

	Kind of temperature		Temperature
[Ref.: #8969]		growth	30 °C
[Ref.: #65225]		optimum	28-37 °C
[Ref.: #65225]	no	growth	10-15 °C
[Ref.: #65225]	no	growth	45 °C
[Ref.: #67770]		growth	28 °C

[Ref.: #8969]	Temperature range	mesophilic
[Ref.: #65225]	Temperature range	mesophilic
[Ref.: #65225]	Temperature range	psychrophilic
[Ref.: #65225]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #65225]			optimum	7
[Ref.: #65225]		no	growth	5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65225]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65225]		NaCl		growth	0.5-4	%

[Ref.: #8969]	Murein short key	B11
[Ref.: #8969]	Murein types	B2delta {Gly} [L-Ala] D-Glu-D-Dab-L-Thr

[Ref.: #67770]

Observation

quinones: MK-11

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65225]	(S)-lactate ChEBI	-	assimilation
[Ref.: #65225]	2,3-butanediol ChEBI	-	assimilation
[Ref.: #65225]	2-deoxyadenosine ChEBI	-	assimilation
[Ref.: #65225]	2-hydroxybutyrate ChEBI	-	assimilation
[Ref.: #65225]	2-oxoglutarate ChEBI	-	assimilation
[Ref.: #65225]	2-oxopentanoate ChEBI	-	assimilation
[Ref.: #65225]	3-O-methyl-D-glucose ChEBI	-	assimilation
[Ref.: #65225]	4-hydroxybutyrate ChEBI	-	assimilation
[Ref.: #65225]	4-hydroxyphenylacetic acid ChEBI	-	assimilation
[Ref.: #65225]	acetate ChEBI	+	assimilation
[Ref.: #65225]	acetoacetate ChEBI	+	assimilation
[Ref.: #65225]	adenosine ChEBI	-	assimilation
[Ref.: #65225]	alpha-cyclodextrin ChEBI	-	assimilation
[Ref.: #65225]	alpha-D-glucose ChEBI	-	assimilation
[Ref.: #65225]	amp ChEBI	-	assimilation
[Ref.: #65225]	amygdalin ChEBI	-	assimilation
[Ref.: #65225]	arbutin ChEBI	-	assimilation
[Ref.: #65225]	beta-cyclodextrin ChEBI	-	assimilation
[Ref.: #65225]	beta-hydroxybutyrate ChEBI	-	assimilation
[Ref.: #65225]	bromosuccinate ChEBI	-	assimilation
[Ref.: #65225]	cellobiose ChEBI	-	assimilation
[Ref.: #65225]	citrate ChEBI	-	assimilation
[Ref.: #65225]	cyclodextrin ChEBI	-	assimilation
[Ref.: #65225]	D-alanine ChEBI	-	assimilation
[Ref.: #65225]	D-arabitol ChEBI	-	assimilation
[Ref.: #65225]	D-aspartate ChEBI	-	assimilation
[Ref.: #65225]	D-fructose ChEBI	-	assimilation
[Ref.: #65225]	D-fructose 6-phosphate ChEBI	-	assimilation
[Ref.: #65225]	D-fucose ChEBI	-	assimilation
[Ref.: #65225]	D-galactose ChEBI	-	assimilation
[Ref.: #65225]	D-galacturonic acid ChEBI	-	assimilation
[Ref.: #65225]	D-gluconate ChEBI	-	assimilation
[Ref.: #65225]	D-glucose 6-phosphate ChEBI	-	assimilation
[Ref.: #65225]	D-glucuronate ChEBI	-	assimilation
[Ref.: #65225]	D-malate ChEBI	-	assimilation
[Ref.: #65225]	D-mannitol ChEBI	-	assimilation
[Ref.: #65225]	D-mannose ChEBI	-	assimilation
[Ref.: #65225]	D-psicose ChEBI	-	assimilation
[Ref.: #65225]	D-ribose ChEBI	-	assimilation
[Ref.: #65225]	D-saccharate ChEBI	-	assimilation
[Ref.: #65225]	D-serine ChEBI	-	assimilation
[Ref.: #65225]	D-sorbitol ChEBI	-	assimilation
[Ref.: #65225]	D-tagatose ChEBI	-	assimilation
[Ref.: #65225]	D-xylose ChEBI	-	assimilation
[Ref.: #65225]	formate ChEBI	-	assimilation
[Ref.: #65225]	galactarate ChEBI	-	assimilation
[Ref.: #65225]	gamma-aminobutyric acid ChEBI	+	assimilation
[Ref.: #65225]	gentiobiose ChEBI	-	assimilation
[Ref.: #65225]	glucose 1-phosphate ChEBI	-	assimilation
[Ref.: #65225]	glucuronamide ChEBI	-	assimilation
[Ref.: #65225]	glycerol ChEBI	-	assimilation
[Ref.: #65225]	glycerol 1-phosphate ChEBI	-	assimilation
[Ref.: #65225]	glycogen ChEBI	-	assimilation
[Ref.: #65225]	glycyl-l-glutamate ChEBI	-	assimilation
[Ref.: #65225]	inosine ChEBI	-	assimilation
[Ref.: #65225]	inulin ChEBI	-	assimilation
[Ref.: #65225]	L-alaninamide ChEBI	+	assimilation
[Ref.: #65225]	L-alanine ChEBI	+	assimilation
[Ref.: #65225]	L-alanylglycine ChEBI	-	assimilation
[Ref.: #65225]	L-arabinose ChEBI	-	assimilation
[Ref.: #65225]	L-arginine ChEBI	+	assimilation
[Ref.: #65225]	L-asparagine ChEBI	-	assimilation
[Ref.: #65225]	L-fucose ChEBI	-	assimilation
[Ref.: #65225]	L-galactonic acid gamma-lactone ChEBI	-	assimilation
[Ref.: #65225]	L-glutamate ChEBI	-	assimilation
[Ref.: #65225]	L-histidine ChEBI	+	assimilation
[Ref.: #65225]	L-malate ChEBI	-	assimilation
[Ref.: #65225]	L-pyroglutamic acid ChEBI	-	assimilation
[Ref.: #65225]	L-rhamnose ChEBI	-	assimilation
[Ref.: #65225]	L-serine ChEBI	-	assimilation
[Ref.: #65225]	lactamide ChEBI	-	assimilation
[Ref.: #65225]	lactose ChEBI	-	assimilation
[Ref.: #65225]	lactulose ChEBI	-	assimilation
[Ref.: #65225]	maltose ChEBI	-	assimilation
[Ref.: #65225]	maltotriose ChEBI	-	assimilation
[Ref.: #65225]	mannan ChEBI	-	assimilation
[Ref.: #65225]	melezitose ChEBI	-	assimilation
[Ref.: #65225]	melibiose ChEBI	-	assimilation
[Ref.: #65225]	methyl (R)-lactate ChEBI	-	assimilation
[Ref.: #65225]	methyl alpha-D-galactoside ChEBI	-	assimilation
[Ref.: #65225]	methyl alpha-D-glucopyranoside ChEBI	-	assimilation
[Ref.: #65225]	methyl alpha-D-mannoside ChEBI	-	assimilation
[Ref.: #65225]	methyl beta-D-galactoside ChEBI	-	assimilation
[Ref.: #65225]	methyl beta-D-glucopyranoside ChEBI	-	assimilation
[Ref.: #65225]	methyl pyruvate ChEBI	+	assimilation
[Ref.: #65225]	monomethyl succinate ChEBI	-	assimilation
[Ref.: #65225]	myo-inositol ChEBI	-	assimilation
[Ref.: #65225]	N-acetyl-D-mannosamine ChEBI	-	assimilation
[Ref.: #65225]	N-acetyl-L-glutamate ChEBI	-	assimilation
[Ref.: #65225]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #65225]	palatinose ChEBI	-	assimilation
[Ref.: #65225]	pectin ChEBI	-	assimilation
[Ref.: #65225]	propionate ChEBI	-	assimilation
[Ref.: #65225]	putrescine ChEBI	+	assimilation
[Ref.: #65225]	pyruvate ChEBI	+	assimilation
[Ref.: #65225]	quinate ChEBI	-	assimilation
[Ref.: #65225]	raffinose ChEBI	-	assimilation
[Ref.: #65225]	salicin ChEBI	-	assimilation
[Ref.: #65225]	sedoheptulosan ChEBI	-	assimilation
[Ref.: #65225]	stachyose ChEBI	-	assimilation
[Ref.: #65225]	succinamate ChEBI	-	assimilation
[Ref.: #65225]	succinate ChEBI	-	assimilation
[Ref.: #65225]	sucrose ChEBI	-	assimilation
[Ref.: #65225]	thymidine ChEBI	-	assimilation
[Ref.: #65225]	thymidine 5'-monophosphate ChEBI	-	assimilation
[Ref.: #65225]	trehalose ChEBI	-	assimilation
[Ref.: #65225]	turanose ChEBI	-	assimilation
[Ref.: #65225]	tween 40 ChEBI	+	assimilation
[Ref.: #65225]	tween 80 ChEBI	-	assimilation
[Ref.: #65225]	uridine ChEBI	-	assimilation
[Ref.: #65225]	uridine 5'-monophosphate ChEBI	-	assimilation
[Ref.: #65225]	xylitol ChEBI	-	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #65225]		catalase	+	1.11.1.6
[Ref.: #65225]		cytochrome oxidase	-	1.9.3.1

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65225]

Fatty acid	Percentage
C15:0 anteiso	30.3
C15:0 iso	25.1
C16:0 iso	20.7
C17:0 anteiso	14.1
C17:0 iso	6.3
C16:0	2.5
C14:0 iso	0.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8969]	Sample type/isolated from	activated sludge
[Ref.: #8969]	Country	Germany
[Ref.: #8969]	Country ISO 3 Code	DEU
[Ref.: #8969]	Continent	Europe

[Ref.: #65225]	Sample type/isolated from	aerated laboratory scale fermenter (temperature: 15-17°C; volume: 20 l; retention time: 10 days) fed with wastes of a yeast factory
[Ref.: #65225]	Sampling date	1982
[Ref.: #65225]	Geographic location (country and/or sea, region)	Botanical Institute of the University Munich
[Ref.: #65225]	Country	Germany
[Ref.: #65225]	Country ISO 3 Code	DEU
[Ref.: #65225]	Continent	Europe
[Ref.: #65225]	Enrichment culture	enriched nutrient agar medium
[Ref.: #65225]	Enrichment culture composition	with 1.0 g glucose, 7.5 g peptone (Merck), 5.0 g meat extract Lab Lemco' (Oxoid), 2.5 g yeast extract (Oxoid), 2.5 g casamino acids (Difco), 5.0 g NaCl, 13 g agar (Merck) in 1 l tap water (pH 7.2-7.4)
[Ref.: #65225]	Enrichment culture temperature	20 ̊C
[Ref.: #65225]	Isolation/enrichment procedure	the plates were incubated until single colonies could be selected for further purification

[Ref.: #67770]	Sample type/isolated from	Aerated laboratory scale fermenter fed with wastes of a yeast factory at the Botanical Institute of the University Munich
[Ref.: #67770]	Country	Germany
[Ref.: #67770]	Country ISO 3 Code	DEU
[Ref.: #67770]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered

#Waste

#Activated sludge

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8969]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8969]	Leucobacter sp. DSM 20621 partial 16S rRNA gene, strain DSM 20621	LT844632	1515	ENA	1983706 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Leucobacter weissii S27	GCA_017565745	contig	GenBank	1983706 tax ID	*

[Ref.: #8969]

GC-content

70.1 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Leucobacter weissii S27 NCBI: GCA_017565745.1
[Ref.: #69480]	flagellated	no	96.524	yes
[Ref.: #69480]	gram-positive	yes	89.109	no
[Ref.: #69480]	anaerobic	no	98.786	no
[Ref.: #69480]	aerobic	yes	87.663	no
[Ref.: #69480]	halophile	no	82.985	no
[Ref.: #69480]	spore-forming	no	94.4	yes
[Ref.: #69480]	thermophile	no	99.578	no
[Ref.: #69480]	glucose-util	yes	63.602	yes
[Ref.: #69480]	motile	no	89.843	yes
[Ref.: #69480]	glucose-ferment	no	89.103	no

Availability in culture collections External links

[Ref.: #8969]

Culture collection no.

DSM 20621, CCM 8762, JCM 32600

[Ref.: #76840]

SI-ID 49660

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Leucobacter weissii sp. nov., an isolate from activated sludge once described as first representative of the peptidoglycan variation B2delta, and emended description of the genus Leucobacter.	Schumann P, Pukall R	Int J Syst Evol Microbiol	10.1099/ijsem.0.002454	2017	*

References

#8969

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20621

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65225

Peter Schumann, Rüdiger Pukall: Leucobacter weissii sp. nov., an isolate from activated sludge once described as first representative of the peptidoglycan variation B2d, and emended description of the genus Leucobacter. IJSEM 67: 5244 - 5251 2017 ( DOI 10.1099/ijsem.0.002454 , PubMed 29087268 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#76840

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID49660.1 )

* These data were automatically processed and therefore are not curated

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