Strain identifier

BacDive ID: 7183

Type strain: Yes

Species: Methylobacterium iners

Strain Designation: 5317S-33

Strain history: KACC 11765 <-- H.-Y. Weon 5317S-33.

NCBI tax ID(s): 418707 (species)

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General

@ref: 7832

BacDive-ID: 7183

DSM-Number: 19015

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Methylobacterium iners 5317S-33 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from air sample.

NCBI tax id

  • NCBI tax id: 418707
  • Matching level: species

strain history

@refhistory
7832<- S.-W. Kwon, KACC; 5317S-33
67770KACC 11765 <-- H.-Y. Weon 5317S-33.

doi: 10.13145/bacdive7183.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Methylobacteriaceae
  • genus: Methylobacterium
  • species: Methylobacterium iners
  • full scientific name: Methylobacterium iners Weon et al. 2008

@ref: 7832

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Methylobacteriaceae

genus: Methylobacterium

species: Methylobacterium iners

full scientific name: Methylobacterium iners Weon et al. 2008

strain designation: 5317S-33

type strain: yes

Morphology

cell morphology

  • @ref: 32155
  • gram stain: negative
  • cell length: 2 µm
  • cell width: 1 µm
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 32155
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 7832
  • name: R2A MEDIUM (DSMZ Medium 830)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/830
  • composition: Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
7832positivegrowth30mesophilic
32155positivegrowth10-30
32155positiveoptimum26.5mesophilic
67770positivegrowth30mesophilic

culture pH

@refabilitytypepH
32155positivegrowth06-08
32155positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 32155
  • oxygen tolerance: aerobe

halophily

  • @ref: 32155
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: <2 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3215530089acetate+carbon source
3215522653asparagine+carbon source
3215535391aspartate+carbon source
3215515740formate+carbon source
3215529987glutamate+carbon source
3215524996lactate+carbon source
3215517272propionate+carbon source
3215551850methyl pyruvate+carbon source
3215530031succinate+carbon source

enzymes

@refvalueactivityec
32155catalase+1.11.1.6
32155cytochrome oxidase+1.9.3.1

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
7832air sampleSuwon cityRepublic of KoreaKORAsia
67770Air sample in Suwon CityRepublic of KoreaKORAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Air

Safety information

risk assessment

  • @ref: 7832
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 7832
  • description: Methylobacterium iners strain 5317S-33 16S ribosomal RNA gene, partial sequence
  • accession: EF174497
  • length: 1353
  • database: ena
  • NCBI tax ID: 418707

Genome sequences

  • @ref: 66792
  • description: Methylobacterium iners DSM 19015
  • accession: GCA_022179305
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 418707

GC content

@refGC-contentmethod
783268.0
3215568
6777068high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno70.344no
gram-positiveno97.918no
anaerobicno97.428yes
aerobicyes90.928yes
halophileno93.617no
spore-formingno93.626no
motileyes86.296no
glucose-fermentno89.938no
thermophileno98.305yes
glucose-utilyes83.985no

External links

@ref: 7832

culture collection no.: DSM 19015, KACC 11765, JCM 16407

straininfo link

  • @ref: 76636
  • straininfo: 323974

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny18175690Methylobacterium iners sp. nov. and Methylobacterium aerolatum sp. nov., isolated from air samples in Korea.Weon HY, Kim BY, Joa JH, Son JA, Song MH, Kwon SW, Go SJ, Yoon SHInt J Syst Evol Microbiol10.1099/ijs.0.65047-02008*Air Microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, Fatty Acids/analysis, Genes, rRNA, Korea, Methylobacterium/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species SpecificityGenetics
Phylogeny21378138Methylobacterium gossipiicola sp. nov., a pink-pigmented, facultatively methylotrophic bacterium isolated from the cotton phyllosphere.Madhaiyan M, Poonguzhali S, Senthilkumar M, Lee JS, Lee KCInt J Syst Evol Microbiol10.1099/ijs.0.030148-02011Bacterial Typing Techniques, Base Composition, Carbon/metabolism, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Gossypium/*microbiology, India, Locomotion, Methylobacterium/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Pigments, Biological/metabolism, Plant Leaves/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAMetabolism
Phylogeny21908681Methylobacterium oxalidis sp. nov., isolated from leaves of Oxalis corniculata.Tani A, Sahin N, Kimbara KInt J Syst Evol Microbiol10.1099/ijs.0.033019-02011Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Ferns/*microbiology, Locomotion, Methylobacterium/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Pigments, Biological/metabolism, Plant Leaves/microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
7832Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19015)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19015
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32155Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2839828776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
76636Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID323974.1StrainInfo: A central database for resolving microbial strain identifiers