Strain identifier
BacDive ID: 7130
Type strain: 
Species: Methanosarcina lacustris
Strain Designation: ZS
Strain history: <- M. V. Simankova, Russian Academy of Sciences, Inst. of Microbiology, Moscow, Russia; ZS <- M. V. Simankova {1999}
NCBI tax ID(s): 170861 (species)
version 8.1 (current version)
General
@ref: 5063
BacDive-ID: 7130
DSM-Number: 13486
keywords: 16S sequence, Archaea, anaerobe, mesophilic
description: Methanosarcina lacustris ZS is an anaerobe, mesophilic archaeon that was isolated from anoxic lake sediment.
NCBI tax id
- NCBI tax id: 170861
- Matching level: species
strain history
- @ref: 5063
- history: <- M. V. Simankova, Russian Academy of Sciences, Inst. of Microbiology, Moscow, Russia; ZS <- M. V. Simankova {1999}
doi: 10.13145/bacdive7130.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanomicrobia
- order: Methanosarcinales
- family: Methanosarcinaceae
- genus: Methanosarcina
- species: Methanosarcina lacustris
- full scientific name: Methanosarcina lacustris Simankova et al. 2002
@ref: 5063
domain: Archaea
phylum: Euryarchaeota
class: Methanomicrobia
order: Methanosarcinales
family: Methanosarcinaceae
genus: Methanosarcina
species: Methanosarcina lacustris
full scientific name: Methanosarcina lacustris Simankova et al. 2002
strain designation: ZS
type strain: yes
Culture and growth conditions
culture medium
- @ref: 5063
- name: METHANOSARCINA BARKERI MEDIUM (DSMZ Medium 120a)
- growth: yes
- link: https://mediadive.dsmz.de/medium/120a
- composition: Name: METHANOSARCINA BARKERI MEDIUM (DSMZ Medium 120a; with strain-specific modifications) Composition: Methanol 3.86719 g/l NaCl 2.19727 g/l NaHCO3 1.95312 g/l Yeast extract 1.95312 g/l Casitone 1.95312 g/l NH4Cl 0.488281 g/l MgSO4 x 7 H2O 0.488281 g/l K2HPO4 0.341797 g/l Na2S x 9 H2O 0.292969 g/l L-Cysteine HCl x H2O 0.292969 g/l CaCl2 x 2 H2O 0.244141 g/l KH2PO4 0.224609 g/l HCl 0.00244141 g/l FeSO4 x 7 H2O 0.00195312 g/l FeCl2 x 4 H2O 0.00146484 g/l Sodium resazurin 0.000488281 g/l CoCl2 x 6 H2O 0.000185547 g/l Pyridoxine hydrochloride 9.76563e-05 g/l MnCl2 x 4 H2O 9.76563e-05 g/l ZnCl2 6.83594e-05 g/l Thiamine HCl 4.88281e-05 g/l Riboflavin 4.88281e-05 g/l Calcium D-(+)-pantothenate 4.88281e-05 g/l Nicotinic acid 4.88281e-05 g/l p-Aminobenzoic acid 4.88281e-05 g/l (DL)-alpha-Lipoic acid 4.88281e-05 g/l Na2MoO4 x 2 H2O 3.51562e-05 g/l NiCl2 x 6 H2O 2.34375e-05 g/l Folic acid 1.95312e-05 g/l Biotin 1.95312e-05 g/l H3BO3 5.85938e-06 g/l CuCl2 x 2 H2O 1.95313e-06 g/l Vitamin B12 9.76563e-07 g/l H2SO4 Distilled water
culture temp
- @ref: 5063
- growth: positive
- type: growth
- temperature: 25
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 5063
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 5063
- sample type: anoxic lake sediment
- country: Switzerland
- origin.country: CHE
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Lake (large) |
| #Environmental | #Aquatic | #Sediment |
| #Condition | #Anoxic (anaerobic) |
Safety information
risk assessment
- @ref: 5063
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 5063
- description: Methanosarcina lacustera 16S ribosomal RNA gene, partial sequence
- accession: AF432127
- length: 1381
- database: ena
- NCBI tax ID: 170861
GC content
- @ref: 5063
- GC-content: 43.4
- method: thermal denaturation, midpoint method (Tm)
External links
@ref: 5063
culture collection no.: DSM 13486, VKM B-2268
straininfo link
- @ref: 76585
- straininfo: 125927
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 11822671 | Methanosarcina lacustris sp. nov., a new psychrotolerant methanogenic archaeon from anoxic lake sediments. | Simankova MV, Parshina SN, Tourova TP, Kolganova TV, Zehnder AJ, Nozhevnikova AN | Syst Appl Microbiol | 10.1078/0723-2020-00058 | 2001 | Base Sequence, Geologic Sediments/*microbiology, Methanosarcina/classification/growth & development/*isolation & purification, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/chemistry, *Water Microbiology | Genetics |
| Phylogeny | 25604340 | Methanosarcina subterranea sp. nov., a methanogenic archaeon isolated from a deep subsurface diatomaceous shale formation. | Shimizu S, Ueno A, Naganuma T, Kaneko K | Int J Syst Evol Microbiol | 10.1099/ijs.0.000072 | 2015 | Base Composition, DNA, Archaeal/genetics, Genes, Archaeal, Groundwater/*microbiology, Japan, Methanosarcina/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Genetics |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 5063 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13486) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-13486 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 76585 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID125927.1 | StrainInfo: A central database for resolving microbial strain identifiers |