General

@ref: 3058

BacDive-ID: 7105

DSM-Number: 7222

keywords: Archaea, anaerobe, mesophilic

description: Methanosarcina mazei Gö1 is an anaerobe, mesophilic archaeon that was isolated from anaerobic sewage digester, derived from strain DSM 3647.

NCBI tax id

• NCBI tax id: 2209
• Matching level: species

strain history

• @ref: 3058
• history: <- R. I. L. Eggen, Wageningen University, Laboratory of Microbiology, Netherlands (Methanosarcina frisia) <- M. Blaut, Universität Göttingen, Germany <- Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH <- F. Widdel, Universität Göttingen, Germany; Gö1 {1978}

doi: 10.13145/bacdive7105.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/archaea
• keyword: phylum/methanobacteriota
• domain: Archaea
• phylum: Methanobacteriota
• class: Methanomicrobia
• order: Methanosarcinales
• family: Methanosarcinaceae
• genus: Methanosarcina
• species: Methanosarcina mazei
• full scientific name: Methanosarcina mazei corrig. (Barker 1936) Mah and Kuhn 1984

• synonyms

  @ref	synonym
  20215	Methanosarcina frisia
  20215	Methanococcus frisius
  20215	Methanosarcina mazeii
  20215	Methanococcus mazei

@ref: 3058

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosarcinaceae

genus: Methanosarcina

species: Methanosarcina mazei

full scientific name: Methanosarcina mazei (Barker 1936) Mah and Kuhn 1984

strain designation: Gö1

type strain: no

Culture and growth conditions

culture medium

• @ref: 3058
• name: METHANOSARCINA MAZEI (GÖ1) MEDIUM (DSMZ Medium 120c)
• growth: yes
• link: https://mediadive.dsmz.de/medium/120c
• composition: Name: METHANOSARCINA MAZEI (GOE1) MEDIUM (DSMZ Medium 120c) Composition: Sucrose 17.0319 g/l Na-acetate 9.96016 g/l NaCl 2.24104 g/l Yeast extract 1.99203 g/l Casitone 1.99203 g/l NaHCO3 1.99203 g/l NH4Cl 0.498008 g/l MgSO4 x 7 H2O 0.498008 g/l K2HPO4 0.348606 g/l Na2S x 9 H2O 0.298805 g/l L-Cysteine HCl x H2O 0.298805 g/l CaCl2 x 2 H2O 0.249004 g/l KH2PO4 0.229084 g/l HCl 0.00249004 g/l FeSO4 x 7 H2O 0.00199203 g/l FeCl2 x 4 H2O 0.00149402 g/l Sodium resazurin 0.000498008 g/l CoCl2 x 6 H2O 0.000189243 g/l Pyridoxine hydrochloride 9.96016e-05 g/l MnCl2 x 4 H2O 9.96016e-05 g/l ZnCl2 6.97211e-05 g/l (DL)-alpha-Lipoic acid 4.98008e-05 g/l Thiamine HCl 4.98008e-05 g/l p-Aminobenzoic acid 4.98008e-05 g/l Calcium D-(+)-pantothenate 4.98008e-05 g/l Nicotinic acid 4.98008e-05 g/l Riboflavin 4.98008e-05 g/l Na2MoO4 x 2 H2O 3.58566e-05 g/l NiCl2 x 6 H2O 2.39044e-05 g/l Biotin 1.99203e-05 g/l Folic acid 1.99203e-05 g/l H3BO3 5.9761e-06 g/l CuCl2 x 2 H2O 1.99203e-06 g/l Vitamin B12 9.96016e-07 g/l H2SO4 Distilled water

culture temp

• @ref: 3058
• growth: positive
• type: growth
• temperature: 37
• range: mesophilic

Physiology and metabolism

oxygen tolerance

• @ref: 3058
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

• @ref: 3058
• sample type: anaerobic sewage digester, derived from strain DSM 3647
• geographic location: Göttingen
• country: Germany
• origin.country: DEU
• continent: Europe

isolation source categories

Safety information

risk assessment

• @ref: 3058
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

External links

@ref: 3058

culture collection no.: DSM 7222

straininfo link

• @ref: 76560
• straininfo: 48388

Reference