Strain identifier
BacDive ID: 7086
Type strain: 
Species: Methanosarcina acetivorans
Strain Designation: C2A
Strain history: <- K.R. Sowers, C2A
NCBI tax ID(s): 2214 (species)
version 8.1 (current version)
General
@ref: 1234
BacDive-ID: 7086
DSM-Number: 2834
keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic
description: Methanosarcina acetivorans C2A is an anaerobe, mesophilic archaeon that was isolated from marine mud.
NCBI tax id
- NCBI tax id: 2214
- Matching level: species
strain history
- @ref: 1234
- history: <- K.R. Sowers, C2A
doi: 10.13145/bacdive7086.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanomicrobia
- order: Methanosarcinales
- family: Methanosarcinaceae
- genus: Methanosarcina
- species: Methanosarcina acetivorans
- full scientific name: Methanosarcina acetivorans Sowers et al. 1986
@ref: 1234
domain: Archaea
phylum: Euryarchaeota
class: Methanomicrobia
order: Methanosarcinales
family: Methanosarcinaceae
genus: Methanosarcina
species: Methanosarcina acetivorans
full scientific name: Methanosarcina acetivorans Sowers et al. 1986
strain designation: C2A
type strain: yes
Culture and growth conditions
culture medium
- @ref: 1234
- name: METHANOCOCCOIDES MEDIUM (DSMZ Medium 141c)
- growth: yes
- link: https://mediadive.dsmz.de/medium/141c
- composition: Name: METHANOCOCCOIDES MEDIUM (DSMZ Medium 141c; with strain-specific modifications) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Methanol 3.87476 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l CaCl2 x 2 H2O 0.000978474 g/l FeSO4 x 7 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l Nicotinic acid 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Biotin 1.95695e-05 g/l Folic acid 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water
culture temp
- @ref: 1234
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 1234
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 1234
- sample type: marine mud
- geographic location: California, near La Jolla
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Marine |
| #Environmental | #Terrestrial | #Mud (Sludge) |
| #Environmental | #Aquatic | #Sediment |
Safety information
risk assessment
- @ref: 1234
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Corynebacterium matruchotii clone OCM095 16S ribosomal RNA gene, partial sequence | GU420746 | 434 | ena | 43768 |
| 20218 | Methanosarcina acetivorans strain DSM 2834 16S ribosomal RNA gene, partial sequence | M59137 | 1426 | ena | 2214 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Methanosarcina acetivorans C2A | GCA_000007345 | complete | ncbi | 188937 |
| 66792 | Methanosarcina acetivorans C2A | 638154508 | complete | img | 2214 |
| 66792 | Methanosarcina acetivorans C2A | 188937.25 | complete | patric | 188937 |
GC content
- @ref: 1234
- GC-content: 41.0
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | no | 91.963 | no |
| flagellated | no | 97.338 | no |
| gram-positive | no | 95.943 | no |
| anaerobic | yes | 96.849 | yes |
| halophile | no | 86.693 | no |
| spore-forming | no | 94.821 | no |
| glucose-util | yes | 73.737 | no |
| aerobic | no | 92.262 | yes |
| thermophile | no | 99.611 | yes |
| glucose-ferment | no | 70.424 | no |
External links
@ref: 1234
culture collection no.: DSM 2834, ATCC 35395, JCM 12185, OCM 95
straininfo link
- @ref: 76542
- straininfo: 45987
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 16346552 | Methanosarcina acetivorans sp. nov., an Acetotrophic Methane-Producing Bacterium Isolated from Marine Sediments. | Sowers KR, Baron SF, Ferry JG | Appl Environ Microbiol | 10.1128/aem.47.5.971-978.1984 | 1984 | ||
| 16534921 | Methanogenic conversion of 3-s-methylmercaptopropionate to 3-mercaptopropionate. | van der Maarel M, Jansen M, Hansen TA | Appl Environ Microbiol | 10.1128/aem.61.1.48-51.1995 | 1995 | |||
| Metabolism | 19413812 | Improved approach for transferring and cultivating Methanosarcina acetivorans C2A (DSM 2834). | Summer H | Lett Appl Microbiol | 10.1111/j.1472-765X.2009.02592.x | 2009 | Culture Media/metabolism, Culture Techniques/*methods, Methanosarcina/*growth & development/metabolism, Microbial Viability, Microbiological Techniques/*methods | Cultivation |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 1234 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2834) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-2834 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 76542 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID45987.1 | StrainInfo: A central database for resolving microbial strain identifiers |