Strain identifier

BacDive ID: 7074

Type strain: Yes

Species: Methanohalophilus portucalensis

Strain Designation: FDF-1

Strain history: <- OCM <- I. M. Mathrani, University of California, School of Public Health, Los Angeles, USA; FDF-1

NCBI tax ID(s): 523843 (strain), 39664 (species)

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General

@ref: 3173

BacDive-ID: 7074

DSM-Number: 7471

keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic

description: Methanohalophilus portucalensis FDF-1 is an anaerobe, mesophilic archaeon that was isolated from sediment of a saltern.

NCBI tax id

NCBI tax idMatching level
39664species
523843strain

strain history

  • @ref: 3173
  • history: <- OCM <- I. M. Mathrani, University of California, School of Public Health, Los Angeles, USA; FDF-1

doi: 10.13145/bacdive7074.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanomicrobia
  • order: Methanosarcinales
  • family: Methanosarcinaceae
  • genus: Methanohalophilus
  • species: Methanohalophilus portucalensis
  • full scientific name: Methanohalophilus portucalensis Boone et al. 1993

@ref: 3173

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosarcinaceae

genus: Methanohalophilus

species: Methanohalophilus portucalensis

full scientific name: Methanohalophilus portucalensis Boone et al. 1993

strain designation: FDF-1

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 3173
  • name: METHANOHALOPHILUS MEDIUM (DSMZ Medium 479)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/479
  • composition: Name: METHANOHALOPHILUS MEDIUM (DSMZ Medium 479) Composition: MgCl2 x 6 H2O 6.0 g/l Trypticase peptone 2.0 g/l Yeast extract 2.0 g/l Trimethylamine-HCl 2.0 g/l Na2CO3 1.5 g/l KCl 1.5 g/l NH4Cl 1.0 g/l K2HPO4 x 3 H2O 0.4 g/l Na2S x 9 H2O 0.25 g/l 2-Mercaptoethanesulfonic acid 0.2 g/l MgSO4 x 7 H2O 0.03 g/l Nitrilotriacetic acid 0.015 g/l NaCl 0.01 g/l MnSO4 x H2O 0.005 g/l ZnSO4 x 7 H2O 0.0018 g/l CoSO4 x 7 H2O 0.0018 g/l CaCl2 x 2 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Sodium resazurin 0.0005 g/l NiCl2 x 6 H2O 0.0003 g/l AlK(SO4)2 x 12 H2O 0.0002 g/l H3BO3 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l CuSO4 x 5 H2O 0.0001 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l Distilled water

culture temp

  • @ref: 3173
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 3173
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 3173
  • sample type: sediment of a saltern
  • geographic location: Figueira da Foz
  • country: Portugal
  • origin.country: PRT
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Engineered#Built environment#Indoor
#Condition#Saline

Safety information

risk assessment

  • @ref: 3173
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Enterococcus faecalis clone OCM059 16S ribosomal RNA gene, partial sequenceGU417240457ena1351
3173Methanohalophilus portucalensis FDF-1 16S ribosomal RNA gene, partial sequenceAY2907171208ena523843

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Methanohalophilus portucalensis FDF-1GCA_900177455contigncbi523843
66792Methanohalophilus portucalensis FDF-1 DSM 7471GCA_003722115contigncbi523843
66792Methanohalophilus portucalensis FDF-1GCA_001888095scaffoldncbi523843
66792Methanohalophilus portucalensis FDF-12914860506draftimg523843
66792Methanohalophilus portucalensis FDF-12706794887draftimg523843
66792Methanohalophilus portucalensis FDF-12806310721completeimg39664
66792Methanohalophilus portucalensis FDF-1 strain DSM 7471523843.7wgspatric523843
66792Methanohalophilus portucalensis FDF-1523843.5wgspatric523843

GC content

  • @ref: 3173
  • GC-content: 41.95
  • method: sequence analysis

External links

@ref: 3173

culture collection no.: DSM 7471, OCM 59

straininfo link

  • @ref: 76531
  • straininfo: 48447

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny24670897Methanohalophilus levihalophilus sp. nov., a slightly halophilic, methylotrophic methanogen isolated from natural gas-bearing deep aquifers, and emended description of the genus Methanohalophilus.Katayama T, Yoshioka H, Mochimaru H, Meng XY, Muramoto Y, Usami J, Ikeda H, Kamagata Y, Sakata SInt J Syst Evol Microbiol10.1099/ijs.0.063677-02014Base Composition, DNA, Archaeal/genetics, Groundwater/*microbiology, Japan, Methanol, Methanosarcinaceae/*classification/genetics/isolation & purification, Molecular Sequence Data, Natural Gas/*microbiology, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAGenetics
Genetics29348351Complete Genome Sequence of the Halophilic Methylotrophic Methanogen Archaeon Methanohalophilus portucalensis Strain FDF-1(T).L'Haridon S, Corre E, Guan Y, Vinu M, La Cono V, Yakimov M, Stingl U, Toffin L, Jebbar MGenome Announc10.1128/genomeA.01482-172018

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3173Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7471)https://www.dsmz.de/collection/catalogue/details/culture/DSM-7471
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
76531Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID48447.1StrainInfo: A central database for resolving microbial strain identifiers