Strain identifier
BacDive ID: 7064
Type strain: 
Species: Methanohalophilus halophilus
Strain Designation: Z-7982
Strain history: <- T.N. Zhilina, Z-7982
NCBI tax ID(s): 2177 (species)
version 8.1 (current version)
General
@ref: 1298
BacDive-ID: 7064
DSM-Number: 3094
keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic, Gram-negative
description: Methanohalophilus halophilus Z-7982 is an anaerobe, mesophilic, Gram-negative archaeon that was isolated from cyanobacterial mat and bottom deposits.
NCBI tax id
- NCBI tax id: 2177
- Matching level: species
strain history
- @ref: 1298
- history: <- T.N. Zhilina, Z-7982
doi: 10.13145/bacdive7064.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanomicrobia
- order: Methanosarcinales
- family: Methanosarcinaceae
- genus: Methanohalophilus
- species: Methanohalophilus halophilus
- full scientific name: Methanohalophilus halophilus (Zhilina 1984) Wilharm et al. 1991
synonyms
- @ref: 20215
- synonym: Methanococcus halophilus
@ref: 1298
domain: Archaea
phylum: Euryarchaeota
class: Methanomicrobia
order: Methanosarcinales
family: Methanosarcinaceae
genus: Methanohalophilus
species: Methanohalophilus halophilus
full scientific name: Methanohalophilus halophilus (Zhilina 1984) Wilharm et al. 1991
strain designation: Z-7982
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: negative
- confidence: 99.985
Culture and growth conditions
culture medium
- @ref: 1298
- name: METHANOHALOPHILUS HALOPHILUS MEDIUM (DSMZ Medium 329)
- growth: yes
- link: https://mediadive.dsmz.de/medium/329
- composition: Name: METHANOHALOPHILUS HALOPHILUS MEDIUM (DSMZ Medium 329) Composition: NaCl 69.8603 g/l Trimethylamine-HCl 4.99002 g/l NaHCO3 4.99002 g/l MgCl2 x 6 H2O 0.329341 g/l CaCl2 x 2 H2O 0.329341 g/l KCl 0.329341 g/l KH2PO4 0.329341 g/l NH4Cl 0.329341 g/l L-Cysteine HCl x H2O 0.299401 g/l Na2S x 9 H2O 0.149701 g/l Yeast extract 0.0499002 g/l K2SO4 0.00998004 g/l HCl 0.00249501 g/l FeCl2 x 4 H2O 0.00149701 g/l Sodium resazurin 0.000499002 g/l CoCl2 x 6 H2O 0.000189621 g/l Pyridoxine hydrochloride 9.98004e-05 g/l MnCl2 x 4 H2O 9.98004e-05 g/l ZnCl2 6.98603e-05 g/l (DL)-alpha-Lipoic acid 4.99002e-05 g/l p-Aminobenzoic acid 4.99002e-05 g/l Calcium D-(+)-pantothenate 4.99002e-05 g/l Thiamine HCl 4.99002e-05 g/l Nicotinic acid 4.99002e-05 g/l Riboflavin 4.99002e-05 g/l Na2MoO4 x 2 H2O 3.59281e-05 g/l NiCl2 x 6 H2O 2.39521e-05 g/l Biotin 1.99601e-05 g/l Folic acid 1.99601e-05 g/l H3BO3 5.98802e-06 g/l CuCl2 x 2 H2O 1.99601e-06 g/l Vitamin B12 9.98004e-07 g/l Distilled water
culture temp
- @ref: 1298
- growth: positive
- type: growth
- temperature: 30
- range: mesophilic
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 1298 | anaerobe | |
| 69480 | anaerobe | 99.078 |
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 100 |
| 69480 | no | 99.921 |
Isolation, sampling and environmental information
isolation
- @ref: 1298
- sample type: cyanobacterial mat and bottom deposits
- geographic location: Shark Bay, Hamelin-Pool
- country: Australia
- origin.country: AUS
- continent: Australia and Oceania
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Microbial community | #Microbial mat |
| #Host | #Microbial | #Bacteria |
taxonmaps
- @ref: 69479
- File name: preview.99_187134.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17802;96_68814;97_89456;98_121731;99_187134&stattab=map
- Last taxonomy: Methanohalophilus
- 16S sequence: FR733671
- Sequence Identity:
- Total samples: 165
- soil counts: 51
- aquatic counts: 111
- animal counts: 2
- plant counts: 1
Safety information
risk assessment
- @ref: 1298
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Methanohalophilus halophilus partial 16S rRNA gene, type strain DSM3094T | FR733671 | 1467 | ena | 2177 |
| 20218 | Methanohalophilus halophilus strain Z-7982 16S ribosomal RNA gene, partial sequence | JQ346755 | 1265 | ena | 2177 |
| 1298 | Methanohalophilus halophilus partial 16S rRNA gene, type strain DSM 3094T | FN870068 | 1467 | ena | 2177 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Methanohalophilus halophilus Z-7982 | GCA_001889405 | complete | ncbi | 2177 |
| 66792 | Methanohalophilus halophilus DSM 3094 | GCA_003722055 | contig | ncbi | 2177 |
| 66792 | Methanohalophilus halophilus Z-7982 | GCA_900106905 | contig | ncbi | 2177 |
| 66792 | Methanohalophilus halophilus Z-7982 | 2642422606 | draft | img | 2177 |
| 66792 | Methanohalophilus halophilus DSM 3094 | 2914862632 | draft | img | 2177 |
| 66792 | Methanohalophilus halophilus strain Z-7982 | 2177.5 | complete | patric | 2177 |
| 66792 | Methanohalophilus halophilus strain DSM 3094 | 2177.7 | wgs | patric | 2177 |
GC content
- @ref: 1298
- GC-content: 42.4
- method: sequence analysis
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| flagellated | no | 97.444 | no |
| gram-positive | no | 97.535 | no |
| anaerobic | yes | 98.052 | yes |
| halophile | no | 67.784 | no |
| spore-forming | no | 96.172 | no |
| glucose-util | yes | 73.594 | no |
| aerobic | no | 93.968 | yes |
| thermophile | no | 87.603 | yes |
| motile | no | 93.567 | no |
| glucose-ferment | no | 73.536 | no |
External links
@ref: 1298
culture collection no.: DSM 3094, NBRC 107635, OCM 160
straininfo link
- @ref: 76521
- straininfo: 47693
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 24670897 | Methanohalophilus levihalophilus sp. nov., a slightly halophilic, methylotrophic methanogen isolated from natural gas-bearing deep aquifers, and emended description of the genus Methanohalophilus. | Katayama T, Yoshioka H, Mochimaru H, Meng XY, Muramoto Y, Usami J, Ikeda H, Kamagata Y, Sakata S | Int J Syst Evol Microbiol | 10.1099/ijs.0.063677-0 | 2014 | Base Composition, DNA, Archaeal/genetics, Groundwater/*microbiology, Japan, Methanol, Methanosarcinaceae/*classification/genetics/isolation & purification, Molecular Sequence Data, Natural Gas/*microbiology, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Genetics |
| Genetics | 28209822 | Complete Genome Sequence of Methanohalophilus halophilus DSM 3094(T), Isolated from a Cyanobacterial Mat and Bottom Deposits at Hamelin Pool, Shark Bay, Northwestern Australia. | L'Haridon S, Corre E, Guan Y, Vinu M, La Cono V, Yakimov M, Stingl U, Toffin L, Jebbar M | Genome Announc | 10.1128/genomeA.01604-16 | 2017 | Phylogeny |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 1298 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3094) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-3094 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 76521 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID47693.1 | StrainInfo: A central database for resolving microbial strain identifiers |