Strain identifier

BacDive ID: 7052

Type strain: Yes

Species: Methanosaeta pelagica

Strain Designation: 03d30q

Strain history: <- K. Mori, NBRC; 03d30q <- K. Mori {2008}

NCBI tax ID(s): 1110545 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 17601

BacDive-ID: 7052

DSM-Number: 24271

keywords: 16S sequence, Archaea, anaerobe, mesophilic

description: Methanosaeta pelagica 03d30q is an anaerobe, mesophilic archaeon that was isolated from tidal flat sediment.

NCBI tax id

  • NCBI tax id: 1110545
  • Matching level: species

strain history

  • @ref: 17601
  • history: <- K. Mori, NBRC; 03d30q <- K. Mori {2008}

doi: 10.13145/bacdive7052.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanomicrobia
  • order: Methanosarcinales
  • family: Methanotrichaceae
  • genus: Methanosaeta
  • species: Methanosaeta pelagica
  • full scientific name: Methanosaeta pelagica Mori et al. 2012

@ref: 17601

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosaetaceae

genus: Methanosaeta

species: Methanosaeta pelagica

full scientific name: Methanosaeta pelagica Mori et al. 2012

strain designation: 03d30q

type strain: yes

Morphology

colony morphology

  • @ref: 17601
  • incubation period: >14 days

Culture and growth conditions

culture medium

  • @ref: 17601
  • name: METHANOSAETA PELAGICA MEDIUM (DSMZ Medium 1329)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1329
  • composition: Name: METHANOSAETA PELAGICA MEDIUM (DSMZ Medium 1329) Composition: Na-acetate 6.54038 g/l MgCl2 x 6 H2O 3.04088 g/l Yeast extract 1.49551 g/l Na2CO3 1.49551 g/l KH2PO4 1.18644 g/l NH4Cl 0.538385 g/l Trypticase peptone 0.398804 g/l Na2S x 9 H2O 0.358923 g/l K2HPO4 0.209372 g/l 2-Mercaptoethanesulfonic acid 0.139581 g/l Nitrilotriacetic acid 0.0255234 g/l FeCl2 x 4 H2O 0.00199402 g/l NaCl 0.00199402 g/l Sodium resazurin 0.000498504 g/l CaCl2 x 2 H2O 0.000199402 g/l MnCl2 x 4 H2O 0.000199402 g/l NiCl2 x 6 H2O 0.000199402 g/l ZnCl2 0.000199402 g/l Pyridoxine hydrochloride 9.97009e-05 g/l Na2WO4 x 2 H2O 7.97607e-05 g/l CoCl2 x 6 H2O 5.98205e-05 g/l Na2MoO4 x 2 H2O 5.98205e-05 g/l Na2SeO3 x 5 H2O 5.98205e-05 g/l Thiamine HCl 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l CuCl2 3.98804e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l H3BO3 1.99402e-05 g/l Vitamin B12 9.97009e-07 g/l Distilled water

culture temp

  • @ref: 17601
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 17601
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 17601
  • sample type: tidal flat sediment
  • geographic location: Chiba Prefecture, Tokyo Bay
  • country: Japan
  • origin.country: JPN
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Tidal flat
#Environmental#Terrestrial#Sediment

taxonmaps

  • @ref: 69479
  • File name: preview.99_194103.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17897;96_71549;97_92978;98_126478;99_194103&stattab=map
  • Last taxonomy: Methanosaeta pelagica subclade
  • 16S sequence: AB679167
  • Sequence Identity:
  • Total samples: 38
  • soil counts: 1
  • aquatic counts: 37

Sequence information

16S sequences

  • @ref: 17601
  • description: Methanosaeta pelagica gene for 16S rRNA, partial sequence
  • accession: AB679167
  • length: 1383
  • database: ena
  • NCBI tax ID: 1110545

GC content

  • @ref: 17601
  • GC-content: 45.4
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 17601

culture collection no.: DSM 24271, NBRC 105920

literature

  • topic: Phylogeny
  • Pubmed-ID: 22344667
  • title: Aceticlastic and NaCl-requiring methanogen "Methanosaeta pelagica" sp. nov., isolated from marine tidal flat sediment.
  • authors: Mori K, Iino T, Suzuki K, Yamaguchi K, Kamagata Y
  • journal: Appl Environ Microbiol
  • DOI: 10.1128/AEM.07484-11
  • year: 2012
  • mesh: Acetates/metabolism, Archaeal Proteins/genetics, Cluster Analysis, DNA, Archaeal/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Geologic Sediments/*microbiology, Methane/*metabolism, Methanosarcinales/*classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitle
17601Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24271)https://www.dsmz.de/collection/catalogue/details/culture/DSM-24271
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta