Strain identifier

BacDive ID: 7049

Type strain: Yes

Species: Methanothrix thermoacetophila

Strain Designation: Z-517

Strain history: <- A. N. Nozhevnikova, Institute of Microbiology, Russ. Academy of Sciences, Moscow, Russia; Z-517 <- A. N. Nozhevnikova and T. G. Yagodina

NCBI tax ID(s): 2224 (species)

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General

@ref: 1818

BacDive-ID: 7049

DSM-Number: 4774

keywords: 16S sequence, Archaea, anaerobe, thermophilic

description: Methanothrix thermoacetophila Z-517 is an anaerobe, thermophilic archaeon that was isolated from mud, thermal Khlorid Lake.

NCBI tax id

  • NCBI tax id: 2224
  • Matching level: species

strain history

  • @ref: 1818
  • history: <- A. N. Nozhevnikova, Institute of Microbiology, Russ. Academy of Sciences, Moscow, Russia; Z-517 <- A. N. Nozhevnikova and T. G. Yagodina

doi: 10.13145/bacdive7049.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanomicrobia
  • order: Methanosarcinales
  • family: Methanotrichaceae
  • genus: Methanothrix
  • species: Methanothrix thermoacetophila
  • full scientific name: Methanothrix thermoacetophila corrig. Nozhevnikova and Chudina 1988

  • synonyms

    @refsynonym
    20215Methanothrix thermophila
    20215Methanosaeta thermophila
    20215Methanothrix thermoacetophilia
    20215Methanosaeta thermoacetophila

@ref: 1818

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosaetaceae

genus: Methanothrix

species: Methanothrix thermoacetophila

full scientific name: Methanothrix thermoacetophila Nozhevnikova and Chudina 1988

strain designation: Z-517

type strain: yes

Morphology

colony morphology

  • @ref: 1818
  • incubation period: 3-7 days

Culture and growth conditions

culture medium

  • @ref: 1818
  • name: METHANOTHRIX SOEHNGENII MEDIUM (DSMZ Medium 334a)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/334a
  • composition: Name: METHANOTHRIX SOEHNGENII MEDIUM (DSMZ Medium 334a; with strain-specific modifications) Composition: Na-acetate 6.8 g/l Na2CO3 1.25 g/l Na2HPO4 x 2 H2O 0.86 g/l Methanol 0.792 g/l Na2S x 9 H2O 0.5 g/l NaCl 0.3 g/l NH4Cl 0.3 g/l KH2PO4 0.22 g/l 2-Mercaptoethanesulfonate 0.14 g/l Nitrilotriacetic acid 0.128 g/l CaCl2 x 2 H2O 0.11 g/l MgCl2 x 6 H2O 0.1 g/l FeCl3 x 6 H2O 0.0135 g/l Butyl vinyl ether 0.005 g/l L-alpha-Phosphatidylcholine 0.005 g/l NiCl2 x 6 H2O 0.0012 g/l MnCl2 x 4 H2O 0.001 g/l ZnCl2 0.001 g/l Na2-EDTA 0.000999999 g/l Sodium resazurin 0.0005 g/l CuCl2 0.0003 g/l CoCl2 x 6 H2O 0.0003 g/l Na2MoO4 x 2 H2O 0.0003 g/l Na2SeO3 x 5 H2O 0.0003 g/l H3BO3 0.0001 g/l Pyridoxine hydrochloride 0.0001 g/l (DL)-alpha-Lipoic acid 5e-05 g/l Riboflavin 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l Calcium D-(+)-pantothenate 5e-05 g/l Nicotinic acid 5e-05 g/l Thiamine HCl 5e-05 g/l Biotin 2e-05 g/l Folic acid 2e-05 g/l Vitamin B12 1e-06 g/l Distilled water

culture temp

  • @ref: 1818
  • growth: positive
  • type: growth
  • temperature: 55
  • range: thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 1818
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 1818
  • sample type: mud, thermal Khlorid Lake
  • geographic location: Kamchatka
  • country: USSR
  • origin.country: RUS
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Environmental#Terrestrial#Mud (Sludge)

taxonmaps

  • @ref: 69479
  • File name: preview.99_188479.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17997;96_69193;97_90295;98_122722;99_188479&stattab=map
  • Last taxonomy: Methanothrix thermoacetophila subclade
  • 16S sequence: LN868388
  • Sequence Identity:
  • Total samples: 58
  • soil counts: 1
  • aquatic counts: 54
  • animal counts: 3

Safety information

risk assessment

  • @ref: 1818
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
2021816S rRNA [Methanothrix thermoacetophila, Z-517, DSM 4774, rRNA Partial, 159 nt, segment 1 of 3]S42717159ena2224
2021816S rRNA [Methanothrix thermoacetophila, Z-517, DSM 4774, rRNA Partial, 194 nt, segment 2 of 3]S42720194ena2224
2021816S rRNA [Methanothrix thermoacetophila, Z-517, DSM 4774, rRNA Partial, 186 nt, segment 3 of 3]S42725186ena2224
1818Methanosaeta thermophila partial 16S rRNA gene, type strain DSM 4774T, isolate Z-517LN8683881475ena2224

External links

@ref: 1818

culture collection no.: DSM 4774, VKM B-1831

straininfo link

  • @ref: 76508
  • straininfo: 47867

literature

  • topic: Phylogeny
  • Pubmed-ID: 1380291
  • title: Characterization of three thermophilic strains of Methanothrix ("Methanosaeta") thermophila sp. nov. and rejection of Methanothrix ("Methanosaeta") thermoacetophila.
  • authors: Kamagata Y, Kawasaki H, Oyaizu H, Nakamura K, Mikami E, Endo G, Koga Y, Yamasato K
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-42-3-463
  • year: 1992
  • mesh: Bacterial Proteins/chemistry, Base Composition, Base Sequence, DNA, Bacterial/chemistry, Electrophoresis, Polyacrylamide Gel, Methanosarcinaceae/*classification/cytology/physiology, Molecular Sequence Data, RNA, Bacterial/chemistry, RNA, Ribosomal, 16S/chemistry, Sequence Homology, Nucleic Acid, Terminology as Topic
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1818Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4774)https://www.dsmz.de/collection/catalogue/details/culture/DSM-4774
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76508Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID47867.1StrainInfo: A central database for resolving microbial strain identifiers