Strain identifier
BacDive ID: 7025
Type strain:
Species: Methanoculleus thermophilus
Strain Designation: FR-4
Strain history: <- J.E. Harris, FR-4
NCBI tax ID(s): 2200 (species)
General
@ref: 1233
BacDive-ID: 7025
DSM-Number: 2832
keywords: 16S sequence, Archaea, anaerobe, thermophilic
description: Methanoculleus thermophilus FR-4 is an anaerobe, thermophilic archaeon that was isolated from mud.
NCBI tax id
- NCBI tax id: 2200
- Matching level: species
strain history
- @ref: 1233
- history: <- J.E. Harris, FR-4
doi: 10.13145/bacdive7025.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanomicrobia
- order: Methanomicrobiales
- family: Methanomicrobiaceae
- genus: Methanoculleus
- species: Methanoculleus thermophilus
- full scientific name: Methanoculleus thermophilus corrig. (Rivard and Smith 1982) Maestrojuán et al. 1990
synonyms
@ref synonym 20215 Methanogenium frittonii 20215 Methanoculleus thermophilicus 20215 Methanogenium thermophilicum
@ref: 1233
domain: Archaea
phylum: Euryarchaeota
class: Methanomicrobia
order: Methanomicrobiales
family: Methanomicrobiaceae
genus: Methanoculleus
species: Methanoculleus thermophilus
full scientific name: Methanoculleus thermophilus (Rivard and Smith 1982) Maestrojuán et al. 1990 emend. Spring et al. 2005
strain designation: FR-4
type strain: no
Culture and growth conditions
culture medium
- @ref: 1233
- name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119)
- growth: yes
- link: https://mediadive.dsmz.de/medium/119
- composition: Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water
culture temp
- @ref: 1233
- growth: positive
- type: growth
- temperature: 55
- range: thermophilic
Physiology and metabolism
oxygen tolerance
- @ref: 1233
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 1233
- sample type: mud
- geographic location: Norfolk, Fritton Lake
- country: United Kingdom
- origin.country: GBR
- continent: Europe
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Mud (Sludge)
taxonmaps
- @ref: 69479
- File name: preview.99_187297.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17869;96_68879;97_89568;98_121859;99_187297&stattab=map
- Last taxonomy: Methanoculleus thermophilus subclade
- 16S sequence: AJ862839
- Sequence Identity:
- Total samples: 48
- soil counts: 11
- aquatic counts: 29
- plant counts: 8
Safety information
risk assessment
- @ref: 1233
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 20218
- description: Methanoculleus thermophilus partial 16S rRNA gene, strain DSM 2832
- accession: AJ862839
- length: 1451
- database: ena
- NCBI tax ID: 2200
GC content
- @ref: 1233
- GC-content: 49.2
External links
@ref: 1233
culture collection no.: DSM 2832, OCM 200
straininfo link
- @ref: 76485
- straininfo: 47667
literature
- topic: Phylogeny
- Pubmed-ID: 15879239
- title: Methanogenium frittonii Harris et al. 1996 is a later synonym of Methanoculleus thermophilus (Rivard and Smith 1982) Maestrojuan et al. 1990.
- authors: Spring S, Schumann P, Sproer C
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.63607-0
- year: 2005
- mesh: Acetates/metabolism, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Genes, rRNA, Methanomicrobiaceae/*classification/genetics/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/pharmacology, Temperature, Terminology as Topic
- topic2: Genetics
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
1233 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2832) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-2832 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
76485 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID47667.1 | StrainInfo: A central database for resolving microbial strain identifiers |