Methanoculleus thermophilus (DSM 2832, OCM 200, FR-4)

Name: Methanoculleus thermophilus (DSM 2832, OCM 200, FR-4)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7025

Type strain

Strain Designation FR-4

Culture col. no. DSM 2832 OCM 200 FR-4

NCBI tax ID(s) 2200

Special Collections DSMZ

Links

Methanoculleus thermophilus FR-4 is an anaerobe, thermophilic prokaryote that was isolated from mud.

anaerobe thermophilic 16S sequence

Name and taxonomic classification

SI-ID 47667

DSM 2832

@ref 20215

Last LPSN update 2025-12-16 09:48:28

Domain Methanobacteriati

Phylum Methanobacteriota

Class "Methanomicrobia"

Order Methanomicrobiales

Family Methanomicrobiaceae

Genus Methanoculleus

Species Methanoculleus thermophilus

Full scientific name Methanoculleus thermophilus corrig. (Rivard and Smith 1982) Maestrojuán et al. 1990

Synonyms (3)

Methanogenium thermophilicum
Methanogenium frittonii
Methanoculleus thermophilicus

BacDive ID	Other strains from **Methanoculleus thermophilus** (4)	Type strain
7022	M. thermophilus CR-1, DSM 2373, ATCC 33837, OCM 174, VKM ... (type strain)
7023	M. thermophilus UCLA, DSM 2624, ATCC 43577, OCM 6
7024	M. thermophilus Ratisbona, DSM 2640
7026	M. thermophilus TCI, DSM 3915, OCM 71

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
1233	METHANOBACTERIUM MEDIUM (DSMZ Medium 119)		Medium recipe at MediaDive	Name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l KH2PO4 0.498504 g/l NH4Cl 0.398804 g/l NaCl 0.398804 g/l MgSO4 x 7 H2O 0.398804 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Isobutyric acid DL-2-Methylbutyric acid Valeric acid Isovaleric acid H2SO4 Sludge Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
1233	positive	growth	55	thermophilic

Physiology and metabolism

Oxygen tolerance

1233

Oxygen toleranceanaerobe

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Mud (Sludge)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
1233	mud	Norfolk, Fritton Lake	United Kingdom	GBR	Europe

Taxonmaps

69479

Global distribution of 16S sequence AJ862839 (>99% sequence identity) for Methanoculleus thermophilus subclade from Microbeatlas

Aquatic Samples	29
Soil Samples	11
Animal Samples
Plant Samples	8
Total Samples	48

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
1233	1	Risk group (German classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Methanoculleus thermophilus partial 16S rRNA gene, strain DSM 2832	AJ862839	1451		2200

GC content

@ref	GC-content (mol%)	Method
1233	56.1	high performance liquid chromatography (HPLC)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methanogenium frittonii Harris et al. 1996 is a later synonym of Methanoculleus thermophilus (Rivard and Smith 1982) Maestrojuan et al. 1990.	Spring S, Schumann P, Sproer C	Int J Syst Evol Microbiol	10.1099/ijs.0.63607-0	2005

References

#1233	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2832
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanoculleus thermophilus (DSM 2832, OCM 200, FR-4)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

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