Strain identifier
BacDive ID: 6989
Type strain: 
Species: Methanococcus maripaludis
Strain Designation: JJ
Strain history: DSM 2067 <-- W. J. Jones JJ.
NCBI tax ID(s): 39152 (species)
version 9.3 (current version)
General
@ref: 701
BacDive-ID: 6989
DSM-Number: 2067
keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic
description: Methanococcus maripaludis JJ is an anaerobe, mesophilic archaeon that was isolated from marine marsh sediment.
NCBI tax id
- NCBI tax id: 39152
- Matching level: species
strain history
| @ref | history |
|---|---|
| 701 | <- W. J. Jones, University of Illinois, Urbana, USA; JJ <- W. J. Jones {1974} |
| 67770 | DSM 2067 <-- W. J. Jones JJ. |
doi: 10.13145/bacdive6989.20250331.9.3
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanococci
- order: Methanococcales
- family: Methanococcaceae
- genus: Methanococcus
- species: Methanococcus maripaludis
- full scientific name: Methanococcus maripaludis Jones et al. 1984
synonyms
- @ref: 20215
- synonym: Methanococcus deltae
@ref: 701
domain: Archaea
phylum: Euryarchaeota
class: Methanococci
order: Methanococcales
family: Methanococcaceae
genus: Methanococcus
species: Methanococcus maripaludis
full scientific name: Methanococcus maripaludis Jones et al. 1984
strain designation: JJ
type strain: yes
Morphology
cell morphology
- @ref: 125439
- gram stain: negative
- confidence: 94.3
Culture and growth conditions
culture medium
- @ref: 701
- name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141)
- growth: yes
- link: https://mediadive.dsmz.de/medium/141
- composition: Name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l FeSO4 x 7 H2O 0.000978474 g/l CaCl2 x 2 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l Nicotinic acid 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Folic acid 1.95695e-05 g/l Biotin 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 701 | positive | growth | 37 |
| 67770 | positive | growth | 37 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 701 | anaerobe | |
| 125439 | facultative anaerobe | 97.8 |
spore formation
- @ref: 125439
- spore formation: no
- confidence: 96
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 701 | marine marsh sediment | South Carolina, Pawley's island | USA | USA | North America |
| 67770 | Salt marsh sediment near Pawley's Island | SC | USA | USA | North America |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Marine |
| #Environmental | #Aquatic | #Sediment |
taxonmaps
- @ref: 69479
- File name: preview.99_191917.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17939;96_69441;97_90195;98_122596;99_191917&stattab=map
- Last taxonomy: Methanococcus
- 16S sequence: KC139247
- Sequence Identity:
- Total samples: 220
- soil counts: 9
- aquatic counts: 93
- animal counts: 118
Safety information
risk assessment
- @ref: 701
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Methanococcus maripaludis culture DSM:2067 16S ribosomal RNA gene, partial sequence | JQ346753 | 1262 | nuccore | 39152 |
| 701 | Methanococcus maripaludis 16S ribosomal RNA, complete sequence | U38484 | 1413 | nuccore | 39152 |
| 67770 | Methanococcus maripaludis strain NBRC 101831 16S ribosomal RNA gene, partial sequence | KC139247 | 1400 | nuccore | 39152 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Methanococcus maripaludis DSM 2067 | GCA_002945325 | complete | ncbi | 39152 |
| 66792 | Methanococcus maripaludis DSM 2067 | 2814123256 | complete | img | 39152 |
| 66792 | Methanococcus maripaludis strain DSM 2067 | 39152.8 | complete | patric | 39152 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 701 | 33 | sequence analysis |
| 67770 | 33.4 | high performance liquid chromatography (HPLC) |
| 67770 | 33 | Buoyant density centrifugation (BD) |
Genome-based predictions
predictions
| @ref | model | trait | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positive | Positive reaction to Gram-staining | no | 62.739 | no |
| 125438 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | yes | 89.353 | yes |
| 125438 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | no | 87.401 | no |
| 125438 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 89.571 | no |
| 125438 | thermophile | thermophilic | Ability to grow at temperatures above or equal to 45°C | no | 69.401 | no |
| 125438 | motile2+ | flagellated | Ability to perform flagellated movement | no | 85.375 | no |
| 125439 | BacteriaNet | spore_formation | Ability to form endo- or exospores | no | 96 | |
| 125439 | BacteriaNet | motility | Ability to perform movement | yes | 67 | |
| 125439 | BacteriaNet | gram_stain | Reaction to gram-staining | negative | 94.3 | |
| 125439 | BacteriaNet | oxygen_tolerance | Oxygenic conditions needed for growth | facultative anaerobe | 97.8 |
External links
@ref: 701
culture collection no.: DSM 2067, ATCC 43000, JCM 10722, NBRC 101831
straininfo link
- @ref: 76450
- straininfo: 41586
literature
- topic: Genetics
- Pubmed-ID: 29622618
- title: Complete Genome Sequence of the Methanococcus maripaludis Type Strain JJ (DSM 2067), a Model for Selenoprotein Synthesis in Archaea.
- authors: Poehlein A, Heym D, Quitzke V, Fersch J, Daniel R, Rother M
- journal: Genome Announc
- DOI: 10.1128/genomeA.00237-18
- year: 2018
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 701 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2067) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-2067 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 76450 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID41586.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
| 125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann | 10.1101/2024.08.12.607695 | Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets | |||
| 125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy | https://github.com/GenomeNet/deepG | deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 |