Methanococcus maripaludis (DSM 2067, ATCC 43000, JCM 10722)

Name: Methanococcus maripaludis (DSM 2067, ATCC 43000, JCM 10722)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6989

Type strain

Strain Designation JJ

Culture col. no. DSM 2067 ATCC 43000 JCM 10722 JJ NBRC 101831

NCBI tax ID(s) 39152

Special Collections DSMZ JCM

Links

Methanococcus maripaludis JJ is an anaerobe, mesophilic prokaryote that was isolated from marine marsh sediment.

anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 41586

ATCC 43000,DSM 2067,JCM 10722,NBRC 101831

@ref 20215

Last LPSN update 2025-12-16 09:48:28

Domain Methanobacteriati

Phylum Methanobacteriota

Class Methanococci

Order Methanococcales

Family Methanococcaceae

Genus Methanococcus

Species Methanococcus maripaludis

Full scientific name Methanococcus maripaludis Jones et al. 1984

Synonyms (1)

Methanococcus deltae

BacDive ID	Other strains from **Methanococcus maripaludis** (5)	Type strain
6990	M. maripaludis Delta RC, DSM 2771, ATCC 35294
6991	M. maripaludis LL, S2, DSM 14266, JCM 13030, NBRC 101832
160836	M. maripaludis JCM 10011, NBRC 102114, OCM 209
160837	M. maripaludis JCM 10012, OCM 363
160838	M. maripaludis JCM 10013, OCM 364

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	94.3

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
701	METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141)		Medium recipe at MediaDive	Name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l FeSO4 x 7 H2O 0.000978474 g/l CaCl2 x 2 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l Nicotinic acid 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Folic acid 1.95695e-05 g/l Biotin 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
701	positive	growth	37	mesophilic
67770	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
701	anaerobe
125439	facultative anaerobe	97.8

Spore formation

@ref	Spore formation	Confidence
125439		96

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
701	marine marsh sediment	South Carolina, Pawley's island	USA	USA	North America
67770	Salt marsh sediment near Pawley's Island	SC	USA	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence KC139247 (>99% sequence identity) for Methanococcus from Microbeatlas

Aquatic Samples	93
Soil Samples	9
Animal Samples	118
Plant Samples
Total Samples	220

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
701	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM294532v1 assembly for Methanococcus maripaludis DSM 2067	complete	GCA_002945325	39152.8	2814123256	39152	96.28

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Methanococcus maripaludis culture DSM:2067 16S ribosomal RNA gene, partial sequence	JQ346753	1262	39152
701	Methanococcus maripaludis 16S ribosomal RNA, complete sequence	U38484	1413	39152
67770	Methanococcus maripaludis strain NBRC 101831 16S ribosomal RNA gene, partial sequence	KC139247	1400	39152

GC content

@ref	GC-content (mol%)	Method
701	33	sequence analysis
67770	33.4	high performance liquid chromatography (HPLC)
67770	33	Buoyant density centrifugation (BD)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Methanococcus maripaludis DSM 2067
NCBI: GCA_002945325

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	96.00	no
125439	motility	BacteriaNetⓘ	yes	67.00	no
125439	gram_stain	BacteriaNetⓘ	negative	94.30	no
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	97.80	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Methanococcus maripaludis DSM 2067
NCBI: GCA_002945325.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	62.74	no
125438	anaerobic	anaerobicⓘ	yes	89.35	yes
125438	aerobic	aerobicⓘ	no	87.40	no
125438	spore-forming	spore-formingⓘ	no	89.57	no
125438	thermophilic	thermophileⓘ	no	69.40	no
125438	flagellated	motile2+ⓘ	no	85.38	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Adaptation of a methanogen to Fe⁰ corrosion via direct contact.	Kawaichi S, Kotoky R, Fiutowski J, Rotaru AE.	NPJ Biofilms Microbiomes	10.1038/s41522-024-00574-w	2024
	Improved Quantitative Real-Time PCR Protocol for Detection and Quantification of Methanogenic Archaea in Stool Samples.	Cisek AA, Bak I, Cukrowska B.	Microorganisms	10.3390/microorganisms11030660	2023
Enzymology	Structure of the ATP-driven methyl-coenzyme M reductase activation complex.	Ramirez-Amador F, Paul S, Kumar A, Lorent C, Keller S, Bohn S, Nguyen T, Lometto S, Vlegels D, Kahnt J, Deobald D, Abendroth F, Vazquez O, Hochberg G, Scheller S, Stripp ST, Schuller JM.	Nature	10.1038/s41586-025-08890-7	2025
Genetics	A Rapid, Sensitive, Low-Cost Assay for Detecting Hydrogenotrophic Methanogens in Anaerobic Digesters Using Loop-Mediated Isothermal Amplification.	Alessi AM, Tao B, Zhang W, Zhang Y, Heaven S, Banks CJ, Chong JPJ.	Microorganisms	10.3390/microorganisms8050740	2020
Enzymology	Localization of Methyl-Coenzyme M reductase as metabolic marker for diverse methanogenic Archaea.	Wrede C, Walbaum U, Ducki A, Heieren I, Hoppert M.	Archaea	10.1155/2013/920241	2013
	Emergence of a "Cyclosome" in a Primitive Network Capable of Building "Infinite" Proteins.	Demongeot J, Norris V.	Life (Basel)	10.3390/life9020051	2019
Metabolism	Discovery of multiple modified F(430) coenzymes in methanogens and anaerobic methanotrophic archaea suggests possible new roles for F(430) in nature.	Allen KD, Wegener G, White RH.	Appl Environ Microbiol	10.1128/aem.02202-14	2014
Metabolism	Inactivation of the selB gene in Methanococcus maripaludis: effect on synthesis of selenoproteins and their sulfur-containing homologs.	Rother M, Mathes I, Lottspeich F, Bock A.	J Bacteriol	10.1128/jb.185.1.107-114.2003	2003
Metabolism	Post-transcriptional modification in archaeal tRNAs: identities and phylogenetic relations of nucleotides from mesophilic and hyperthermophilic Methanococcales.	McCloskey JA, Graham DE, Zhou S, Crain PF, Ibba M, Konisky J, Soll D, Olsen GJ.	Nucleic Acids Res	10.1093/nar/29.22.4699	2001
Metabolism	Lysine-2,3-aminomutase and beta-lysine acetyltransferase genes of methanogenic archaea are salt induced and are essential for the biosynthesis of Nepsilon-acetyl-beta-lysine and growth at high salinity.	Pfluger K, Baumann S, Gottschalk G, Lin W, Santos H, Muller V.	Appl Environ Microbiol	10.1128/aem.69.10.6047-6055.2003	2003
Metabolism	Pathway of glycogen metabolism in Methanococcus maripaludis.	Yu JP, Ladapo J, Whitman WB.	J Bacteriol	10.1128/jb.176.2.325-332.1994	1994
Enzymology	Characterization of enzymes of the branched-chain amino acid biosynthetic pathway in Methanococcus spp.	Xing RY, Whitman WB.	J Bacteriol	10.1128/jb.173.6.2086-2092.1991	1991
	Growth and plating efficiency of methanococci on agar media.	Jones WJ, Whitman WB, Fields RD, Wolfe RS.	Appl Environ Microbiol	10.1128/aem.46.1.220-226.1983	1983
	New insight into RNase P RNA structure from comparative analysis of the archaeal RNA.	Harris JK, Haas ES, Williams D, Frank DN, Brown JW.	RNA	10.1017/s1355838201001777	2001
Enzymology	ADP-dependent phosphofructokinases in mesophilic and thermophilic methanogenic archaea.	Verhees CH, Tuininga JE, Kengen SW, Stams AJ, van der Oost J, de Vos WM.	J Bacteriol	10.1128/jb.183.24.7145-7153.2001	2001
Metabolism	Mercury methylation by the methanogen Methanospirillum hungatei.	Yu RQ, Reinfelder JR, Hines ME, Barkay T.	Appl Environ Microbiol	10.1128/aem.01556-13	2013
Metabolism	Glyceraldehyde-3-phosphate ferredoxin oxidoreductase from Methanococcus maripaludis.	Park MO, Mizutani T, Jones PR.	J Bacteriol	10.1128/jb.00828-07	2007
Metabolism	Mercury methylation by interspecies hydrogen and acetate transfer between sulfidogens and methanogens.	Pak K, Bartha R.	Appl Environ Microbiol	10.1128/aem.64.6.1987-1990.1998	1998
Metabolism	Iron-corroding methanogen isolated from a crude-oil storage tank.	Uchiyama T, Ito K, Mori K, Tsurumaru H, Harayama S.	Appl Environ Microbiol	10.1128/aem.00668-09	2010
Genetics	Complete Genome Sequence of the Methanococcus maripaludis Type Strain JJ (DSM 2067), a Model for Selenoprotein Synthesis in Archaea.	Poehlein A, Heym D, Quitzke V, Fersch J, Daniel R, Rother M	Genome Announc	10.1128/genomeA.00237-18	2018

References

#701	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2067
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanococcus maripaludis (DSM 2067, ATCC 43000, JCM 10722)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_002945325

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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