Archive BacDiveID:10.13145/bacdive6969.20230509.8.1

Strain identifier

BacDive ID: 6969

Type strain:

Species: Methanobrevibacter oralis

Strain Designation: Milano

Strain history: DSM 7256 <-- T. Brusa strain ZR.

NCBI tax ID(s): 66851 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 3075

BacDive-ID: 6969

DSM-Number: 7256

keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic

description: Methanobrevibacter oralis Milano is an anaerobe, mesophilic archaeon that was isolated from human oral cavity.

NCBI tax id

NCBI tax id: 66851
Matching level: species

strain history

@ref	history
3075	<- T. Brusa, University of Milan, Milan, Italy; Milano <- T. Brusa {1991}
67770	DSM 7256 <-- T. Brusa strain ZR.

doi: 10.13145/bacdive6969.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/archaea
keyword: phylum/methanobacteriota
domain: Archaea
phylum: Methanobacteriota
class: Methanobacteria
order: Methanobacteriales
family: Methanobacteriaceae
genus: Methanobrevibacter
species: Methanobrevibacter oralis
full scientific name: Methanobrevibacter oralis Ferrari et al. 1995

@ref: 3075

domain: Archaea

phylum: Euryarchaeota

class: Methanobacteria

order: Methanobacteriales

family: Methanobacteriaceae

genus: Methanobrevibacter

species: Methanobrevibacter oralis

full scientific name: Methanobrevibacter oralis Ferrari et al. 1995

strain designation: Milano

type strain: yes

Culture and growth conditions

culture medium

@ref: 3075
name: METHANOBREVIBACTER MEDIUM (DSMZ Medium 119a)
growth: yes
link: https://mediadive.dsmz.de/medium/119a
composition: Name: METHANOBREVIBACTER MEDIUM (DSMZ Medium 119a) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Yeast extract 0.997009 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l KH2PO4 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l MgSO4 x 7 H2O 0.398804 g/l NaCl 0.398804 g/l NH4Cl 0.398804 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l H2SO4 DL-2-Methylbutyric acid Valeric acid Isovaleric acid Isobutyric acid Distilled water

culture temp

@ref	growth	type	temperature	range
3075	positive	growth	35	mesophilic
67770	positive	growth	35	mesophilic

Physiology and metabolism

oxygen tolerance

@ref: 3075
oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@ref	sample type	geographic location	country	origin.country	continent
3075	human oral cavity	Milan	Italy	ITA	Europe
67770	Human subgingival plaque

isolation source categories

Cat1	Cat2	Cat3
#Host	#Human
#Host Body-Site	#Oral cavity and airways
#Host Body-Site	#Oral cavity and airways	#Mouth

taxonmaps

@ref: 69479
File name: preview.99_187152.png
url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17907;96_68800;97_89442;98_121713;99_187152&stattab=map
Last taxonomy: Methanobrevibacter oralis
16S sequence: HE654003
Sequence Identity:
Total samples: 10979
soil counts: 175
aquatic counts: 215
animal counts: 10434
plant counts: 155

Safety information

risk assessment

@ref: 3075
biosafety level: 2
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref	description	accession	length	database	NCBI tax ID
20218	Methanobrevibacter oralis partial SSU rRNA, phylotype 1	AJ001709	599	ena	66851
3075	Methanobrevibacter oralis partial 16S rRNA gene, type strain DSM 7256T	HE654003	1475	ena	66851

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Methanobrevibacter oralis DSM7256	2684622741	draft	img	66851
67770	Methanobrevibacter oralis DSM 7256	GCA_001639275	contig	ncbi	66851
66792	Methanobrevibacter oralis strain DSM 7256	66851.6	wgs	patric	66851

GC content

@ref	GC-content	method
3075	27.7	sequence analysis
67770	28	thermal denaturation, midpoint method (Tm)

External links

@ref: 3075

culture collection no.: DSM 7256, JCM 30027

straininfo link

@ref: 76431
straininfo: 48390

literature

topic: Genetics
Pubmed-ID: 27340077
title: Draft Genome Sequences of Methanobrevibacter curvatus DSM11111, Methanobrevibacter cuticularis DSM11139, Methanobrevibacter filiformis DSM11501, and Methanobrevibacter oralis DSM7256.
authors: Poehlein A, Seedorf H
journal: Genome Announc
DOI: 10.1128/genomeA.00617-16
year: 2016

Reference

@id	authors	catalogue	doi/url	title	journal	pubmed
3075	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7256)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-7256
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.		10.1016/j.syapm.2013.11.002	StrainInfo introduces electronic passports for microorganisms.	Syst Appl Microbiol. 37: 42-50 2014	24321274
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770	Curators of the JCM		https://jcm.brc.riken.jp/en/
69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.		https://microbeatlas.org/	MicrobeAtlas 1.0 beta
76431	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID48390.1	StrainInfo: A central database for resolving microbial strain identifiers