Strain identifier
BacDive ID: 6969
Type strain: 
Species: Methanobrevibacter oralis
Strain Designation: Milano
Strain history: DSM 7256 <-- T. Brusa strain ZR.
NCBI tax ID(s): 66851 (species)
version 9 (current version)
General
@ref: 3075
BacDive-ID: 6969
DSM-Number: 7256
keywords: genome sequence, 16S sequence, Archaea, anaerobe
description: Methanobrevibacter oralis Milano is an anaerobe archaeon that was isolated from human oral cavity.
NCBI tax id
- NCBI tax id: 66851
- Matching level: species
strain history
| @ref | history |
|---|---|
| 3075 | <- T. Brusa, University of Milan, Milan, Italy; Milano <- T. Brusa {1991} |
| 67770 | DSM 7256 <-- T. Brusa strain ZR. |
doi: 10.13145/bacdive6969.20240510.9
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanobacteria
- order: Methanobacteriales
- family: Methanobacteriaceae
- genus: Methanobrevibacter
- species: Methanobrevibacter oralis
- full scientific name: Methanobrevibacter oralis Ferrari et al. 1995
@ref: 3075
domain: Archaea
phylum: Euryarchaeota
class: Methanobacteria
order: Methanobacteriales
family: Methanobacteriaceae
genus: Methanobrevibacter
species: Methanobrevibacter oralis
full scientific name: Methanobrevibacter oralis Ferrari et al. 1995
strain designation: Milano
type strain: yes
Culture and growth conditions
culture medium
- @ref: 3075
- name: METHANOBREVIBACTER MEDIUM (DSMZ Medium 119a)
- growth: yes
- link: https://mediadive.dsmz.de/medium/119a
- composition: Name: METHANOBREVIBACTER MEDIUM (DSMZ Medium 119a) Composition: NaHCO3 3.98804 g/l Na-formate 1.99402 g/l Yeast extract 0.997009 g/l Na-acetate 0.997009 g/l Na2S x 9 H2O 0.498504 g/l KH2PO4 0.498504 g/l L-Cysteine HCl x H2O 0.498504 g/l MgSO4 x 7 H2O 0.398804 g/l NaCl 0.398804 g/l NH4Cl 0.398804 g/l CaCl2 x 2 H2O 0.0498504 g/l HCl 0.00249252 g/l FeSO4 x 7 H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l H2SO4 DL-2-Methylbutyric acid Valeric acid Isovaleric acid Isobutyric acid Distilled water
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 3075 | positive | growth | 35 |
| 67770 | positive | growth | 35 |
Physiology and metabolism
oxygen tolerance
- @ref: 3075
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 3075 | human oral cavity | Milan | Italy | ITA | Europe |
| 67770 | Human subgingival plaque |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Human | |
| #Host Body-Site | #Oral cavity and airways | |
| #Host Body-Site | #Oral cavity and airways | #Mouth |
taxonmaps
- @ref: 69479
- File name: preview.99_187152.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17907;96_68800;97_89442;98_121713;99_187152&stattab=map
- Last taxonomy: Methanobrevibacter oralis
- 16S sequence: HE654003
- Sequence Identity:
- Total samples: 10979
- soil counts: 175
- aquatic counts: 215
- animal counts: 10434
- plant counts: 155
Safety information
risk assessment
- @ref: 3075
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Methanobrevibacter oralis partial SSU rRNA, phylotype 1 | AJ001709 | 599 | nuccore | 66851 |
| 3075 | Methanobrevibacter oralis partial 16S rRNA gene, type strain DSM 7256T | HE654003 | 1475 | nuccore | 66851 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Methanobrevibacter oralis DSM7256 | 2684622741 | draft | img | 66851 |
| 67770 | Methanobrevibacter oralis DSM 7256 | GCA_001639275 | contig | ncbi | 66851 |
| 66792 | Methanobrevibacter oralis strain DSM 7256 | 66851.6 | wgs | patric | 66851 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 3075 | 27.7 | sequence analysis |
| 67770 | 28 | thermal denaturation, midpoint method (Tm) |
External links
@ref: 3075
culture collection no.: DSM 7256, JCM 30027
straininfo link
- @ref: 76431
- straininfo: 48390
literature
- topic: Genetics
- Pubmed-ID: 27340077
- title: Draft Genome Sequences of Methanobrevibacter curvatus DSM11111, Methanobrevibacter cuticularis DSM11139, Methanobrevibacter filiformis DSM11501, and Methanobrevibacter oralis DSM7256.
- authors: Poehlein A, Seedorf H
- journal: Genome Announc
- DOI: 10.1128/genomeA.00617-16
- year: 2016
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 3075 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7256) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-7256 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 76431 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID48390.1 | StrainInfo: A central database for resolving microbial strain identifiers |