Strain identifier

BacDive ID: 6878

Type strain: Yes

Species: Listeria welshimeri

Strain Designation: V8

Strain history: CIP <- 1981, J. Rocourt, Inst. Pasteur, Paris, France <- H. Seeliger, Würzburg, Germany: strain SLCC 5334, Listeria monocytogenes <- H.J. Welshimer: strain V8

NCBI tax ID(s): 1643 (species)

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General

@ref: 8870

BacDive-ID: 6878

DSM-Number: 20650

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive

description: Listeria welshimeri V8 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from decaying plant material.

NCBI tax id

  • NCBI tax id: 1643
  • Matching level: species

strain history

@refhistory
8870<- CIP <- H.J. Welshimer, V8
67770CIP 81.49 <-- J. Rocourt; Inst. Pasteur, France <-- H. P. R. Seeliger; Germany: SLCC 5334 <-- H. J. Welshimer; V8.
123944CIP <- 1981, J. Rocourt, Inst. Pasteur, Paris, France <- H. Seeliger, Würzburg, Germany: strain SLCC 5334, Listeria monocytogenes <- H.J. Welshimer: strain V8

doi: 10.13145/bacdive6878.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Listeriaceae
  • genus: Listeria
  • species: Listeria welshimeri
  • full scientific name: Listeria welshimeri Rocourt and Grimont 1983

@ref: 8870

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Listeriaceae

genus: Listeria

species: Listeria welshimeri

full scientific name: Listeria welshimeri Rocourt and Grimont 1983

strain designation: V8

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
123944positiverod-shapedyes

colony morphology

  • @ref: 8870
  • type of hemolysis: gamma
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8870COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8870BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
33529MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
123944CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
8870positivegrowth37mesophilic
33529positivegrowth30mesophilic
46270positivegrowth30mesophilic
67770positivegrowth30mesophilic
123944positivegrowth10-41
123944nogrowth5psychrophilic
123944nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 123944
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 98.805

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate+builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol+builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose+builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol+builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside+builds acid from320061
68371methyl alpha-D-mannoside+builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
123944esculin+hydrolysis4853
123944nitrate-reduction17632
123944nitrite-reduction16301
123944sodium thiosulfate-builds gas from132112
123944nitrate+respiration17632
68379nitrate-reduction17632
68379esculin+hydrolysis4853
68379urea-hydrolysis16199
68379gelatin-hydrolysis5291
68379D-ribose-fermentation16988
68379D-xylose+fermentation65327
68379D-mannitol-fermentation16899
68379maltose+fermentation17306
68379lactose+fermentation17716
68379sucrose+fermentation17992
68379glycogen-fermentation28087
68376esculin+hydrolysis4853
68376methyl alpha-D-glucopyranoside+builds acid from320061
68376D-ribose-builds acid from16988
68376glucose 1-phosphate-builds acid from29042
68376D-tagatose+builds acid from16443
68381arginine-hydrolysis29016
68381D-ribose-builds acid from16988
68381D-mannitol-builds acid from16899
68381sorbitol-builds acid from30911
68381trehalose+builds acid from27082
68381raffinose-builds acid from16634
68381sucrose-builds acid from17992
68381L-arabinose-builds acid from30849
68381alpha-cyclodextrin+builds acid from40585
68381glycogen-builds acid from28087
68381pullulan-builds acid from27941
68381maltose+builds acid from17306
68381melibiose-builds acid from28053
68381melezitose-builds acid from6731
68381methyl beta-D-glucopyranoside+builds acid from320055
68381D-tagatose+builds acid from16443
68381urea-hydrolysis16199

antibiotic resistance

  • @ref: 123944
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
12394435581indoleno
6838115688acetoinyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin+
12394415688acetoin+

enzymes

@refvalueactivityec
8870catalase+1.11.1.6
8870cytochrome-c oxidase-1.9.3.1
68381urease-3.5.1.5
68381beta-mannosidase+3.2.1.25
68381N-acetyl-beta-glucosaminidase+3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase-3.5.3.6
68376alpha-mannosidase+3.2.1.24
68376beta-glucosidase+3.2.1.21
68379catalase+1.11.1.6
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase+3.5.1.B15
123944oxidase-
123944beta-galactosidase-3.2.1.23
123944alcohol dehydrogenase-1.1.1.1
123944gelatinase-
123944amylase-
123944catalase+1.11.1.6
123944lecithinase-
123944lysine decarboxylase-4.1.1.18
123944ornithine decarboxylase-4.1.1.17
123944urease-3.5.1.5

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlRIBXYLMANMALLACSACGLYGCAT
46270-+-+--+++----+-+++-+

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8870------+----+++------++++++++++-+-----++--+--+---+

API LIST

@refDIMESCalpha MANDARLXYLRHAMDGRIBG1PTAGbeta HEM
8870++++++/-+--+-
8870+++++-+--+
46270+++---+--+-
46270++++--+--+-

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
46270-+-------++---+++---+++--+--+++-
46270-+--------+----++---+----+--+++-

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
8870decaying plant material
46270Decaying vegetationUSAUSANorth America
67770Decaying vegetation in the United States
123944Decaying vegetationUnited States of AmericaUSANorth America

isolation source categories

  • Cat1: #Host
  • Cat2: #Plants
  • Cat3: #Decomposing plant

taxonmaps

  • @ref: 69479
  • File name: preview.99_71.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_32;96_57;97_60;98_65;99_71&stattab=map
  • Last taxonomy: Listeria monocytogenes
  • 16S sequence: AJ515519
  • Sequence Identity:
  • Total samples: 28471
  • soil counts: 991
  • aquatic counts: 1408
  • animal counts: 25369
  • plant counts: 703

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
88701Risk group (German classification)
1239441Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Listeria welshimeri strain ATCC 35897 16S ribosomal RNA, tRNA-Ile, and tRNA-Ala genes, complete sequence; and 23S ribosomal RNA gene, partial sequenceDQ0658462433ena1643
20218Listeria welshimeri 16S and 23S ribosomal RNA genes, partial sequence, Ile-tRNA and Ala-tRNA genes, complete sequenceU57917588ena1643
20218Listeria welshimeri 16S ribosomal RNA gene, partial sequence, 16S/23S ribosomal RNA intergenic spacer region, complete sequence and 23S ribosomal RNA gene, partial sequenceU78982334ena1643
20218L.welshimeri DNA for 16S ribosomal RNAX985321417ena1643
20218L.welshimeri gene for 16S ribosomal RNAX561491491ena1643
8870Listeria welshimeri partial 16S rRNA gene, type strain CECT 919TAJ5155191002ena1643

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Listeria welshimeri NCTC11857GCA_900187315completencbi1643
66792Listeria welshimeri strain NCTC118571643.7completepatric1643
66792Listeria welshimeri NCTC 118572773857882completeimg1643
67770Listeria welshimeri serovar 6b str. SLCC5334GCA_000060285completencbi386043

GC content

@refGC-contentmethod
887036.0
6777036thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes65no
motileyes73.458no
flagellatedno55.973no
gram-positiveyes93.564no
anaerobicno90.299no
aerobicno91.045no
halophileno52.622no
spore-formingno60.65no
thermophileno99.393yes
glucose-utilyes86.659no
glucose-fermentyes65.088no

External links

@ref: 8870

culture collection no.: DSM 20650, ATCC 35897, CIP 8149, SLCC 5334, CCUG 15529, CIP 81.49, NCTC 11857, CECT 919, CCM 3971, JCM 32816, BCRC 14851, LMG 11389, NCAIM B.01872

straininfo link

  • @ref: 76342
  • straininfo: 3533

literature

  • topic: Pathogenicity
  • Pubmed-ID: 22653914
  • title: Rugulactone and its analogues exert antibacterial effects through multiple mechanisms including inhibition of thiamine biosynthesis.
  • authors: Nodwell MB, Menz H, Kirsch SF, Sieber SA
  • journal: Chembiochem
  • DOI: 10.1002/cbic.201200265
  • year: 2012
  • mesh: Amino Acid Sequence, Anti-Bacterial Agents/chemical synthesis/*chemistry/pharmacology, Bacterial Proteins/antagonists & inhibitors/metabolism, Binding Sites, Cryptocarya/chemistry, Lactones/chemical synthesis/*chemistry/pharmacology, Listeria monocytogenes/drug effects, Mass Spectrometry, Molecular Sequence Data, Proteomics, Staphylococcus aureus/drug effects, Thiamine/*biosynthesis
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8870Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20650)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20650
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33529Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/11211
46270Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 15529)https://www.ccug.se/strain?id=15529
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68376Automatically annotated from API LIST
68379Automatically annotated from API Coryne
68381Automatically annotated from API rID32STR
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
76342Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3533.1StrainInfo: A central database for resolving microbial strain identifiers
123944Curators of the CIPCollection of Institut Pasteur (CIP 81.49)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2081.49