Strain identifier

BacDive ID: 6878

Type strain: Yes

Species: Listeria welshimeri

Strain Designation: V8

Strain history: CIP <- 1981, J. Rocourt, Inst. Pasteur, Paris, France <- H. Seeliger, Würzburg, Germany: strain SLCC 5334, Listeria monocytogenes <- H.J. Welshimer: strain V8

NCBI tax ID(s): 1643 (species)

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General

@ref: 8870

BacDive-ID: 6878

DSM-Number: 20650

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, motile, rod-shaped

description: Listeria welshimeri V8 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from decaying plant material.

NCBI tax id

  • NCBI tax id: 1643
  • Matching level: species

strain history

@refhistory
8870<- CIP <- H.J. Welshimer, V8
67770CIP 81.49 <-- J. Rocourt; Inst. Pasteur, France <-- H. P. R. Seeliger; Germany: SLCC 5334 <-- H. J. Welshimer; V8.
123944CIP <- 1981, J. Rocourt, Inst. Pasteur, Paris, France <- H. Seeliger, Würzburg, Germany: strain SLCC 5334, Listeria monocytogenes <- H.J. Welshimer: strain V8

doi: 10.13145/bacdive6878.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Listeriaceae
  • genus: Listeria
  • species: Listeria welshimeri
  • full scientific name: Listeria welshimeri Rocourt and Grimont 1983

@ref: 8870

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Listeriaceae

genus: Listeria

species: Listeria welshimeri

full scientific name: Listeria welshimeri Rocourt and Grimont 1983

strain designation: V8

type strain: yes

Morphology

cell morphology

  • @ref: 123944
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 8870
  • type of hemolysis: gamma
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8870COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8870BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
33529MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
123944CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperature
8870positivegrowth37
33529positivegrowth30
46270positivegrowth30
67770positivegrowth30
123944positivegrowth10-41
123944nogrowth5
123944nogrowth45

Physiology and metabolism

oxygen tolerance

  • @ref: 123944
  • oxygen tolerance: facultative anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838116199urea-hydrolysis
68381320055methyl beta-D-glucopyranoside+builds acid from
683816731melezitose-builds acid from
6838128053melibiose-builds acid from
6838117306maltose+builds acid from
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838130849L-arabinose-builds acid from
6838117992sucrose-builds acid from
6838127082trehalose+builds acid from
6838116634raffinose-builds acid from
6838130911sorbitol-builds acid from
6838116988D-ribose-builds acid from
6838129016arginine-hydrolysis
6837616443D-tagatose+builds acid from
6837616988D-ribose-builds acid from
68376320061methyl alpha-D-glucopyranoside+builds acid from
683764853esculin+hydrolysis
6837917992sucrose+fermentation
6837928087glycogen-fermentation
6837917716lactose+fermentation
6837917306maltose+fermentation
6837916899D-mannitol-fermentation
6837916988D-ribose-fermentation
683795291gelatin-hydrolysis
6837916199urea-hydrolysis
6837917632nitrate-reduction
683794853esculin+hydrolysis
1239444853esculin+hydrolysis
12394417632nitrate-reduction
12394416301nitrite-reduction
123944132112sodium thiosulfate-builds gas from
12394417632nitrate+respiration
68371Potassium 5-ketogluconate+builds acid from
6837118305arbutin+builds acid from
6837127613amygdalin+builds acid from
68371320061methyl alpha-D-glucopyranoside+builds acid from
6837117754glycerol-builds acid from
6838116443D-tagatose+builds acid from
6838140585alpha-cyclodextrin+builds acid from
6838116899D-mannitol-builds acid from
6837629042glucose 1-phosphate-builds acid from
6837965327D-xylose+fermentation
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol+builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose+builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose+builds acid from
6837117151xylitol+builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837159640N-acetylglucosamine+builds acid from
6837143943methyl alpha-D-mannoside+builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose+builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from

antibiotic resistance

  • @ref: 123944
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
6838115688acetoinyes
12394435581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin+
12394415688acetoin+

enzymes

@refvalueactivityec
8870catalase+1.11.1.6
8870cytochrome-c oxidase-1.9.3.1
68381beta-mannosidase+3.2.1.25
68381urease-3.5.1.5
68381N-acetyl-beta-glucosaminidase+3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381beta-glucuronidase-3.2.1.31
68381alpha-galactosidase-3.2.1.22
68381arginine dihydrolase-3.5.3.6
123944oxidase-
123944beta-galactosidase-3.2.1.23
123944alcohol dehydrogenase-1.1.1.1
123944gelatinase-
123944amylase-
123944catalase+1.11.1.6
123944lecithinase-
123944lysine decarboxylase-4.1.1.18
123944ornithine decarboxylase-4.1.1.17
123944urease-3.5.1.5
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68381alkaline phosphatase-3.1.3.1
68381beta-glucosidase+3.2.1.21
68376alpha-mannosidase+3.2.1.24
68376beta-glucosidase+3.2.1.21
68379catalase+1.11.1.6
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379alpha-glucosidase+3.2.1.20
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase+3.5.1.B15

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlRIBXYLMANMALLACSACGLYGCAT
46270-+-+--+++----+-+++-+

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8870------+----+++------++++++++++-+-----++--+--+---+

API LIST

@refDIMESCalpha MANDARLXYLRHAMDGRIBG1PTAGbeta HEM
8870++++++/-+--+-
8870+++++-+--+
46270+++---+--+-
46270++++--+--+-

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
46270-+-------++---+++---+++--+--+++-
46270-+--------+----++---+----+--+++-

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
8870decaying plant material
46270Decaying vegetationUSAUSANorth America
67770Decaying vegetation in the United States
123944Decaying vegetationUnited States of AmericaUSANorth America

isolation source categories

  • Cat1: #Host
  • Cat2: #Plants
  • Cat3: #Decomposing plant

taxonmaps

  • @ref: 69479
  • File name: preview.99_71.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_32;96_57;97_60;98_65;99_71&stattab=map
  • Last taxonomy: Listeria monocytogenes
  • 16S sequence: AJ515519
  • Sequence Identity:
  • Total samples: 28471
  • soil counts: 991
  • aquatic counts: 1408
  • animal counts: 25369
  • plant counts: 703

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
88701Risk group (German classification)
1239441Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Listeria welshimeri strain ATCC 35897 16S ribosomal RNA, tRNA-Ile, and tRNA-Ala genes, complete sequence; and 23S ribosomal RNA gene, partial sequenceDQ0658462433nuccore1643
20218Listeria welshimeri 16S and 23S ribosomal RNA genes, partial sequence, Ile-tRNA and Ala-tRNA genes, complete sequenceU57917588nuccore1643
20218Listeria welshimeri 16S ribosomal RNA gene, partial sequence, 16S/23S ribosomal RNA intergenic spacer region, complete sequence and 23S ribosomal RNA gene, partial sequenceU78982334nuccore1643
20218L.welshimeri DNA for 16S ribosomal RNAX985321417nuccore1643
20218L.welshimeri gene for 16S ribosomal RNAX561491491nuccore1643
8870Listeria welshimeri partial 16S rRNA gene, type strain CECT 919TAJ5155191002nuccore1643

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Listeria welshimeri NCTC11857GCA_900187315completencbi1643
66792Listeria welshimeri strain NCTC118571643.7completepatric1643
66792Listeria welshimeri NCTC 118572773857882completeimg1643
67770Listeria welshimeri serovar 6b str. SLCC5334GCA_000060285completencbi386043

GC content

@refGC-contentmethod
887036.0
6777036thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingyes81.553no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no90.239yes
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no88.825yes
125438spore-formingspore-formingAbility to form endo- or exosporesyes54.074no
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno94.517no
125438motile2+flagellatedAbility to perform flagellated movementyes64.939no
125439BacteriaNetspore_formationAbility to form endo- or exosporesno60.7
125439BacteriaNetmotilityAbility to perform movementno52.7
125439BacteriaNetgram_stainReaction to gram-stainingpositive67.3
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthmicroaerophile89.6

External links

@ref: 8870

culture collection no.: DSM 20650, ATCC 35897, CIP 8149, SLCC 5334, CCUG 15529, CIP 81.49, NCTC 11857, CECT 919, CCM 3971, JCM 32816, BCRC 14851, LMG 11389, NCAIM B.01872

straininfo link

  • @ref: 76342
  • straininfo: 3533

literature

  • topic: Pathogenicity
  • Pubmed-ID: 22653914
  • title: Rugulactone and its analogues exert antibacterial effects through multiple mechanisms including inhibition of thiamine biosynthesis.
  • authors: Nodwell MB, Menz H, Kirsch SF, Sieber SA
  • journal: Chembiochem
  • DOI: 10.1002/cbic.201200265
  • year: 2012
  • mesh: Amino Acid Sequence, Anti-Bacterial Agents/chemical synthesis/*chemistry/pharmacology, Bacterial Proteins/antagonists & inhibitors/metabolism, Binding Sites, Cryptocarya/chemistry, Lactones/chemical synthesis/*chemistry/pharmacology, Listeria monocytogenes/drug effects, Mass Spectrometry, Molecular Sequence Data, Proteomics, Staphylococcus aureus/drug effects, Thiamine/*biosynthesis
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8870Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20650)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20650
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33529Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/11211
46270Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 15529)https://www.ccug.se/strain?id=15529
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68376Automatically annotated from API LIST
68379Automatically annotated from API Coryne
68381Automatically annotated from API rID32STR
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76342Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3533.1StrainInfo: A central database for resolving microbial strain identifiers
123944Curators of the CIPCollection of Institut Pasteur (CIP 81.49)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2081.49
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann10.1101/2024.08.12.607695Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardyhttps://github.com/GenomeNet/deepGdeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1