Strain identifier

BacDive ID: 6751

Type strain: Yes

Species: Legionella parisiensis

Strain Designation: PF-209C-C2

Strain history: CIP <- 1993, R.F. Benson, CDC: strain PF-209C-C2

NCBI tax ID(s): 45071 (species)

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General

@ref: 7974

BacDive-ID: 6751

DSM-Number: 19216

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, human pathogen

description: Legionella parisiensis PF-209C-C2 is a microaerophile, mesophilic, Gram-negative human pathogen that was isolated from water in cooling tower.

NCBI tax id

  • NCBI tax id: 45071
  • Matching level: species

strain history

@refhistory
7974<- H. E. Müller <- ATCC <- G. W. Gorman; PF-209C-C2
67770GIFU 11745 <-- ATCC 35299 <-- G. W. Gorman PF-209C-C2.
121598CIP <- 1993, R.F. Benson, CDC: strain PF-209C-C2

doi: 10.13145/bacdive6751.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella parisiensis
  • full scientific name: Legionella parisiensis Brenner et al. 1985

@ref: 7974

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella parisiensis

full scientific name: Legionella parisiensis Brenner et al. 1985

strain designation: PF-209C-C2

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.988
121598negativerod-shapedyes

colony morphology

@refincubation period
79742-3 days
497062 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7974BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
34425MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
121598CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
7974positivegrowth37mesophilic
34425positivegrowth37mesophilic
49706positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 7974
  • oxygen tolerance: microaerophile

spore formation

@refspore formationconfidence
69481no100
69480no99.951

enzymes

@refvalueactivityec
7974catalase+1.11.1.6
7974cytochrome-c oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
121598oxidase-
121598catalase+1.11.1.6

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121598-+++-++---++--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
7974water in cooling towerParisFranceFRAEurope
49706Water cooling tower1981
67770Water in cooling towerFranceFRAEurope
121598Other, Cooling tower waterParisFranceFRAEurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Cooling tower
#Environmental#Aquatic

taxonmaps

  • @ref: 69479
  • File name: preview.99_2879.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_70;97_584;98_923;99_2879&stattab=map
  • Last taxonomy: Legionella parisiensis
  • 16S sequence: Z49731
  • Sequence Identity:
  • Total samples: 88
  • soil counts: 31
  • aquatic counts: 32
  • animal counts: 24
  • plant counts: 1

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
7974yes, in single cases1Risk group (German classification)
1215982Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Legionella parisiensis strain MDC1587 16S ribosomal RNA gene, partial sequenceJF720396524ena45071
20218L.parisiensis gene for ribosomal RNA, small subunitZ497311405ena45071
7974Legionella parisiensis 16S ribosomal RNA gene, complete sequenceU596971347ena45071
67770Legionella parisiensis JCM 7561 gene for 16S ribosomal RNA, partial sequenceLC5040391466ena45071

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella parisiensis PF-209C-C2GCA_900639925scaffoldncbi45071
66792Legionella parisiensis strain DSM 1921645071.7wgspatric45071
66792Legionella parisiensis strain NCTC1198345071.8wgspatric45071
66792Legionella parisiensis strain PF-209-C-C245071.6wgspatric45071
66792Legionella parisiensis DSM 192162916396677draftimg45071
67770Legionella parisiensis DSM 19216GCA_001736145contigncbi45071
67770Legionella parisiensis NCTC11983GCA_900461585contigncbi45071

GC content

  • @ref: 67770
  • GC-content: 42
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno64.808no
gram-positiveno98.331no
anaerobicno98.377no
aerobicno87.928yes
halophileno90.836no
spore-formingno92.29no
thermophileno99.196yes
glucose-utilyes69.97no
motileyes86.845no
glucose-fermentno89.307no

External links

@ref: 7974

culture collection no.: DSM 19216, ATCC 35299, CCUG 29670, CIP 103847, NCTC 11983, JCM 7561

straininfo link

  • @ref: 76221
  • straininfo: 46780

literature

  • topic: Metabolism
  • Pubmed-ID: 27904811
  • title: Legionella shows a diverse secondary metabolism dependent on a broad spectrum Sfp-type phosphopantetheinyl transferase.
  • authors: Tobias NJ, Ahrendt T, Schell U, Miltenberger M, Hilbi H, Bode HB
  • journal: PeerJ
  • DOI: 10.7717/peerj.2720
  • year: 2016

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7974Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19216)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19216
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34425Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15690
49706Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 29670)https://www.ccug.se/strain?id=29670
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76221Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46780.1StrainInfo: A central database for resolving microbial strain identifiers
121598Curators of the CIPCollection of Institut Pasteur (CIP 103847)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103847