Name: Legionella parisiensis PF-209C-C2
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 6751

Type strain:

Species: Legionella parisiensis

Strain Designation: PF-209C-C2

Culture col. no.: DSM 19216, ATCC 35299, CCUG 29670, CIP 103847, NCTC 11983, JCM 7561

Strain history: CIP <- 1993, R.F. Benson, CDC: strain PF-209C-C2

NCBI tax ID(s): 45071 (species)

SI-ID 46780

ATCC 35299, NCTC 11983, CCUG 29670, CIP 103847, JCM 7561, DSM 19216

Special Collections

Section

When using BacDive for research please cite our paper

Legionella parisiensis PF-209C-C2 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from water in cooling tower.

Gram-negative
motile
rod-shaped
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Legionellales*
	Family	*Legionellaceae*
	Genus	*Legionella*
	Species	**Legionella parisiensis**
	Full Scientific Name (LPSN)	Legionella parisiensis Brenner et al. 1985

Information on morphological and physiological properties Morphology

[Ref.: #121598]	Gram stain	negative
[Ref.: #125439]	Gram stain	negative Confidence in %99.7
[Ref.: #125438]	Gram stain	negative Confidence in %98.994

[Ref.: #121598]	Cell shape	rod-shaped

[Ref.: #121598]	Motility	yes

[Ref.: #7974]	Incubation period	2-3 days

[Ref.: #49706]	Incubation period	2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7974]

Culture medium

BCYE-AGAR (DSMZ Medium 585)

[Ref.: #7974]

Culture medium growth

[Ref.: #7974]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7974]

Culture medium composition

expand / minimize

[Ref.: #34425]

Culture medium

MEDIUM 23 - for Afipia and Legionella

[Ref.: #34425]

Culture medium growth

[Ref.: #34425]

Culture medium composition

expand / minimize

[Ref.: #121598]

Culture medium

CIP Medium 23

[Ref.: #121598]

Culture medium growth

[Ref.: #121598]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #7974]	growth	37 °C
[Ref.: #34425]	growth	37 °C
[Ref.: #49706]	growth	37 °C
[Ref.: #67770]	growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #7974]	Oxygen tolerance	microaerophile
[Ref.: #125439]	Oxygen tolerance	obligate aerobe Confidence in %99.3

[Ref.: #125439]

Ability of spore formation

no Confidence in %99.5

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #7974]	catalase	+	1.11.1.6
[Ref.: #121598]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #7974]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121598]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121598]	7698	-	+	+	+	-	+	+	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7974]	Sample type/isolated from	water in cooling tower
[Ref.: #7974]	Geographic location (country and/or sea, region)	Paris
[Ref.: #7974]	Country	France
[Ref.: #7974]	Country ISO 3 Code	FRA
[Ref.: #7974]	Continent	Europe

[Ref.: #49706]	Sample type/isolated from	Water cooling tower
[Ref.: #49706]	Sampling date	1981

[Ref.: #67770]	Sample type/isolated from	Water in cooling tower
[Ref.: #67770]	Country	France
[Ref.: #67770]	Country ISO 3 Code	FRA
[Ref.: #67770]	Continent	Europe

[Ref.: #121598]	Sample type/isolated from	Other, Cooling tower water
[Ref.: #121598]	Geographic location (country and/or sea, region)	Paris
[Ref.: #121598]	Country	France
[Ref.: #121598]	Country ISO 3 Code	FRA
[Ref.: #121598]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Industrial	#Cooling tower
#Environmental	#Aquatic	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence Z49731 (>99% sequence identity) for Legionella parisiensis from Microbeatlas

Aquatic Samples	32
Soil Samples	31
Animal Samples	24
Plant Samples	1
Total Samples	88

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7974]	Pathogenicity (human)	yes, in single cases
[Ref.: #7974]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121598]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Legionella parisiensis JCM 7561 gene for 16S ribosomal RNA, partial sequence	LC504039	1466	GenBank ENA	45071 tax ID
[Ref.: #7974]	Legionella parisiensis 16S ribosomal RNA gene, complete sequence	U59697	1347	GenBank ENA	45071 tax ID
[Ref.: #20218]	L.parisiensis gene for ribosomal RNA, small subunit	Z49731	1405	GenBank ENA	45071 tax ID	*
[Ref.: #20218]	Legionella parisiensis strain MDC1587 16S ribosomal RNA gene, partial sequence	JF720396	524	GenBank ENA	45071 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Legionella parisiensis DSM 19216	GCA_001736145	contig	GenBank ENA	45071 tax ID
[Ref.: #67770]	Legionella parisiensis NCTC11983	GCA_900461585	contig	GenBank ENA	45071 tax ID
[Ref.: #66792]	Legionella parisiensis PF-209C-C2	GCA_900639925	scaffold	GenBank ENA	45071 tax ID	*
[Ref.: #66792]	Legionella parisiensis strain DSM 19216	45071.7	wgs	PATRIC	45071 tax ID	*
[Ref.: #66792]	Legionella parisiensis strain NCTC11983	45071.8	wgs	PATRIC	45071 tax ID	*
[Ref.: #66792]	Legionella parisiensis strain PF-209-C-C2	45071.6	wgs	PATRIC	45071 tax ID	*

[Ref.: #67770]

GC-content

42 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %	In training data
	Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Legionella parisiensis PF-209C-C2 NCBI: GCA_900639925.1
[Ref.: #125438]	gram-positive	gram-positiveⓘ	no	98.994	no
[Ref.: #125438]	anaerobic	anaerobicⓘ	no	97.086	no
[Ref.: #125438]	aerobic	aerobicⓘ	no	75.798	yes
[Ref.: #125438]	spore-forming	spore-formingⓘ	no	88.763	no
[Ref.: #125438]	thermophile	thermophilicⓘ	no	95.274	yes
[Ref.: #125438]	motile2+	flagellatedⓘ	yes	64.215	no

	Model	Trait	Prediction	Confidence in %
	Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Legionella parisiensis PF-209C-C2 NCBI: GCA_900639925
[Ref.: #125439]	BacteriaNet	spore_formationⓘ	no	99.5
[Ref.: #125439]	BacteriaNet	motilityⓘ	yes	60.8
[Ref.: #125439]	BacteriaNet	gram_stainⓘ	negative	99.7
[Ref.: #125439]	BacteriaNet	oxygen_toleranceⓘ	obligate aerobe	99.3

Availability in culture collections External links

[Ref.: #7974]

Culture collection no.

DSM 19216, ATCC 35299, CCUG 29670, CIP 103847, NCTC 11983, JCM 7561

[Ref.: #76221]

SI-ID 46780

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Legionella shows a diverse secondary metabolism dependent on a broad spectrum Sfp-type phosphopantetheinyl transferase.	Tobias NJ, Ahrendt T, Schell U, Miltenberger M, Hilbi H, Bode HB	PeerJ	10.7717/peerj.2720	2016	*

References

#7974

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19216

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#34425 ; Curators of the CIP;
#49706 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 29670

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#76221

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID46780.1 )

#121598 Collection of Institut Pasteur ; Curators of the CIP; CIP 103847

#125438

Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )

#125439

Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

* These data were automatically processed and therefore are not curated

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