Strain identifier

BacDive ID: 6743

Type strain: Yes

Species: Legionella oakridgensis

Strain history: CIP <- 1998, R.F. Benson, CDC, Atlanta, USA: strain Oak Ridge 10 <- W.B. Cherry <- R. Tyndall

NCBI tax ID(s): 1268635 (strain), 29423 (species)

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General

@ref: 15581

BacDive-ID: 6743

DSM-Number: 21215

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative

description: Legionella oakridgensis DSM 21215 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from cooling tower.

NCBI tax id

NCBI tax idMatching level
1268635strain
29423species

strain history

@refhistory
15581<- CIP; Oak Ridge 10 {2008} <- CDC <- R. L. Tyndall
121375CIP <- 1998, R.F. Benson, CDC, Atlanta, USA: strain Oak Ridge 10 <- W.B. Cherry <- R. Tyndall

doi: 10.13145/bacdive6743.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella oakridgensis
  • full scientific name: Legionella oakridgensis Orrison et al. 1983

@ref: 15581

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella oakridgensis

full scientific name: Legionella oakridgensis Orrison et al. 1983

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.994
121375negativerod-shapedno

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15581BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
34429MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
121375CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
15581positivegrowth37mesophilic
34429positivegrowth37mesophilic
46478positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
46478microaerophile
121375obligate aerobe

spore formation

@refspore formationconfidence
69481no99
69480no99.939

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12137517632nitrate-reduction
12137516301nitrite-reduction

metabolite production

  • @ref: 121375
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
15581catalase+1.11.1.6
15581cytochrome-c oxidase-1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
121375oxidase-
121375catalase-1.11.1.6
121375urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121375-+++-++---++--------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic location
15581cooling towerUSAUSANorth America
46478Water,industrial cooling towerUSAUSANorth AmericaPennsylvania
121375Environment, Industrial cooling tower waterUnited States of AmericaUSANorth AmericaPennsylvania

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Industrial
  • Cat3: #Cooling tower

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
155812Risk group (German classification)
1213752Risk group (French classification)

Sequence information

16S sequences

  • @ref: 15581
  • description: L.oakridgenesis (ATCC 33761) gene for 16S rRNA
  • accession: X73397
  • length: 1467
  • database: ena
  • NCBI tax ID: 29423

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella oakridgensis ATCC 33761 = DSM 21215 ATCC 33761 = DSM 21215; OR-10GCA_000512355completencbi1268635
66792Legionella oakridgensis ATCC 33761GCA_001648605scaffoldncbi29423
66792Legionella oakridgensis NCTC11531GCA_900636495completencbi29423
66792Legionella oakridgensis ATCC 33761 = DSM 212151268635.3completepatric1268635
66792Legionella oakridgensis ATCC 33761 = DSM 21215 strain ATCC 33761 = DSM 21215; OR-101268635.5plasmidpatric1268635
66792Legionella oakridgensis RV-2-20071274403.3wgspatric1274403
66792Legionella oakridgensis strain ATCC 3376129423.6wgspatric29423
66792Legionella oakridgensis strain NCTC1153129423.7completepatric29423
66792Legionella oakridgensis strain Oak Ridge-1029423.5wgspatric29423
66792Legionella oakridgensis NCTC115312848175811completeimg29423
66792Legionella oakridgensis ATCC 337612545824788completeimg1268635

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno99no
motileno76.574no
flagellatedno96.591no
gram-positiveno98.486no
anaerobicno97.822no
halophileno77.907no
spore-formingno93.348no
thermophileno98.702no
glucose-utilyes73.356no
aerobicno91.676yes
glucose-fermentno86.658no

External links

@ref: 15581

culture collection no.: DSM 21215, ATCC 33761, CCUG 16414, CIP 103884, NCTC 11531, Oak Ridge 10

straininfo link

  • @ref: 76213
  • straininfo: 45962

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny21398499Legionella nagasakiensis sp. nov., isolated from water samples and from a patient with pneumonia.Yang G, Benson RF, Ratcliff RM, Brown EW, Steigerwalt AG, Thacker WL, Daneshvar MI, Morey RE, Saito A, Fields BSInt J Syst Evol Microbiol10.1099/ijs.0.027193-02011Aged, Bacterial Proteins/genetics, Bacterial Typing Techniques, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Female, Fresh Water/*microbiology, Genes, rRNA, Humans, Japan/epidemiology, Legionella/*classification/genetics/*isolation & purification/physiology, Legionellosis/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidylprolyl Isomerase/genetics, Phylogeny, Pneumonia, Bacterial/*microbiology, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, South Australia/epidemiology, Species Specificity, United States/epidemiology, *Water SupplyPathogenicity
Genetics23932911Legionella oakridgensis ATCC 33761 genome sequence and phenotypic characterization reveals its replication capacity in amoebae.Brzuszkiewicz E, Schulz T, Rydzewski K, Daniel R, Gillmaier N, Dittmann C, Holland G, Schunder E, Lautner M, Eisenreich W, Luck C, Heuner KInt J Med Microbiol10.1016/j.ijmm.2013.07.0032013Amoeba/*microbiology, Base Composition, DNA, Bacterial/*chemistry/*genetics, Genes, Bacterial, *Genome, Bacterial, Humans, Legionella/*genetics/*growth & development/isolation & purification, Legionnaires' Disease/microbiology, Molecular Sequence Data, *Sequence Analysis, DNAPathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
15581Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21215)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21215
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
34429Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15730
46478Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 16414)https://www.ccug.se/strain?id=16414
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76213Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID45962.1StrainInfo: A central database for resolving microbial strain identifiers
121375Curators of the CIPCollection of Institut Pasteur (CIP 103884)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103884