Name: Legionella oakridgensis DSM 21215
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 6743

Type strain:

Species: Legionella oakridgensis

Culture col. no.: DSM 21215, ATCC 33761, CCUG 16414, CIP 103884, NCTC 11531, Oak Ridge 10

Strain history: CIP <- 1998, R.F. Benson, CDC, Atlanta, USA: strain Oak Ridge 10 <- W.B. Cherry <- R. Tyndall

NCBI tax ID(s): 1268635 (strain), 29423 (species)

SI-ID 45962

ATCC 33761, CIP 103884, NCTC 11531, CCUG 16414, DSM 21215

Special Collections

Other strains from Legionella oakridgensis

Section

Legionella oakridgensis DSM 21215 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from cooling tower.

Gram-negative
rod-shaped
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Legionellales*
	Family	*Legionellaceae*
	Genus	*Legionella*
	Species	**Legionella oakridgensis**
	Full Scientific Name (LPSN)	Legionella oakridgensis Orrison et al. 1983

Information on morphological and physiological properties Morphology

[Ref.: #121375]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %98.985

[Ref.: #121375]	Cell shape	rod-shaped

[Ref.: #121375]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15581]

Culture medium

BCYE-AGAR (DSMZ Medium 585)

[Ref.: #15581]

Culture medium growth

[Ref.: #15581]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15581]

Culture medium composition

expand / minimize

[Ref.: #34429]

Culture medium

MEDIUM 23 - for Afipia and Legionella

[Ref.: #34429]

Culture medium growth

[Ref.: #34429]

Culture medium composition

expand / minimize

[Ref.: #121375]

Culture medium

CIP Medium 23

[Ref.: #121375]

Culture medium growth

[Ref.: #121375]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #15581]	growth	37 °C
[Ref.: #34429]	growth	37 °C
[Ref.: #46478]	growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #46478]	Oxygen tolerance	microaerophile
[Ref.: #121375]	Oxygen tolerance	obligate aerobe

[Ref.: #69481]

Ability of spore formation

no Confidence in %99

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #121375]		nitrate ChEBI	-	reduction
[Ref.: #121375]		nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #121375]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #15581]	catalase	+	1.11.1.6
[Ref.: #121375]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #15581]	cytochrome-c oxidase	-	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121375]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #121375]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121375]	7726	-	+	+	+	-	+	+	-	-	-	+	+	-	-	-	-	-	-	-	-

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	ethanol fermentation	100	2 of 2
[Ref.: #66794]	cardiolipin biosynthesis	100	7 of 7
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	ubiquinone biosynthesis	100	7 of 7
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	biotin biosynthesis	100	4 of 4
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	palmitate biosynthesis	95.45	21 of 22
[Ref.: #66794]	Entner Doudoroff pathway	90	9 of 10
[Ref.: #66794]	aspartate and asparagine metabolism	88.89	8 of 9
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	CO2 fixation in Crenarchaeota	88.89	8 of 9
[Ref.: #66794]	lipid A biosynthesis	88.89	8 of 9
[Ref.: #66794]	valine metabolism	88.89	8 of 9
[Ref.: #66794]	NAD metabolism	83.33	15 of 18
[Ref.: #66794]	vitamin B6 metabolism	81.82	9 of 11
[Ref.: #66794]	proline metabolism	81.82	9 of 11
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	hydrogen production	80	4 of 5
[Ref.: #66794]	tetrahydrofolate metabolism	78.57	11 of 14
[Ref.: #66794]	photosynthesis	78.57	11 of 14
[Ref.: #66794]	vitamin B1 metabolism	76.92	10 of 13
[Ref.: #66794]	phenylalanine metabolism	76.92	10 of 13
[Ref.: #66794]	sulfopterin metabolism	75	3 of 4
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
[Ref.: #66794]	CMP-KDO biosynthesis	75	3 of 4
[Ref.: #66794]	ppGpp biosynthesis	75	3 of 4
[Ref.: #66794]	flavin biosynthesis	73.33	11 of 15
[Ref.: #66794]	alanine metabolism	72.41	21 of 29
[Ref.: #66794]	lysine metabolism	71.43	30 of 42
[Ref.: #66794]	glycolysis	70.59	12 of 17
[Ref.: #66794]	propionate fermentation	70	7 of 10
[Ref.: #66794]	threonine metabolism	70	7 of 10
[Ref.: #66794]	leucine metabolism	69.23	9 of 13
[Ref.: #66794]	pyrimidine metabolism	68.89	31 of 45
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	d-mannose degradation	66.67	6 of 9
[Ref.: #66794]	acetyl CoA biosynthesis	66.67	2 of 3
[Ref.: #66794]	serine metabolism	66.67	6 of 9
[Ref.: #66794]	formaldehyde oxidation	66.67	2 of 3
[Ref.: #66794]	cyanate degradation	66.67	2 of 3
[Ref.: #66794]	lipid metabolism	64.52	20 of 31
[Ref.: #66794]	glutamate and glutamine metabolism	64.29	18 of 28
[Ref.: #66794]	glutathione metabolism	64.29	9 of 14
[Ref.: #66794]	heme metabolism	64.29	9 of 14
[Ref.: #66794]	pentose phosphate pathway	63.64	7 of 11
[Ref.: #66794]	C4 and CAM-carbon fixation	62.5	5 of 8
[Ref.: #66794]	isoleucine metabolism	62.5	5 of 8
[Ref.: #66794]	gluconeogenesis	62.5	5 of 8
[Ref.: #66794]	histidine metabolism	62.07	18 of 29
[Ref.: #66794]	purine metabolism	61.7	58 of 94
[Ref.: #66794]	methylglyoxal degradation	60	3 of 5
[Ref.: #66794]	cellulose degradation	60	3 of 5
[Ref.: #66794]	non-pathway related	57.89	22 of 38
[Ref.: #66794]	reductive acetyl coenzyme A pathway	57.14	4 of 7
[Ref.: #66794]	citric acid cycle	57.14	8 of 14
[Ref.: #66794]	tyrosine metabolism	57.14	8 of 14
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	53.85	7 of 13
[Ref.: #66794]	oxidative phosphorylation	53.85	49 of 91
[Ref.: #66794]	tryptophan metabolism	52.63	20 of 38
[Ref.: #66794]	polyamine pathway	52.17	12 of 23
[Ref.: #66794]	acetate fermentation	50	2 of 4
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	50	6 of 12
[Ref.: #66794]	phenylmercury acetate degradation	50	1 of 2
[Ref.: #66794]	myo-inositol biosynthesis	50	5 of 10
[Ref.: #66794]	pantothenate biosynthesis	50	3 of 6
[Ref.: #66794]	butanoate fermentation	50	2 of 4
[Ref.: #66794]	ketogluconate metabolism	50	4 of 8
[Ref.: #66794]	cyclohexanol degradation	50	2 of 4
[Ref.: #66794]	quinate degradation	50	1 of 2
[Ref.: #66794]	arginine metabolism	50	12 of 24
[Ref.: #66794]	cis-vaccenate biosynthesis	50	1 of 2
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	50	1 of 2
[Ref.: #66794]	glycogen biosynthesis	50	2 of 4
[Ref.: #66794]	sulfate reduction	46.15	6 of 13
[Ref.: #66794]	cysteine metabolism	44.44	8 of 18
[Ref.: #66794]	propanol degradation	42.86	3 of 7
[Ref.: #66794]	methionine metabolism	42.31	11 of 26
[Ref.: #66794]	3-phenylpropionate degradation	40	6 of 15
[Ref.: #66794]	glycine metabolism	40	4 of 10
[Ref.: #66794]	glycogen metabolism	40	2 of 5
[Ref.: #66794]	lipoate biosynthesis	40	2 of 5
[Ref.: #66794]	coenzyme M biosynthesis	40	4 of 10
[Ref.: #66794]	dTDPLrhamnose biosynthesis	37.5	3 of 8
[Ref.: #66794]	degradation of sugar alcohols	37.5	6 of 16
[Ref.: #66794]	isoprenoid biosynthesis	34.62	9 of 26
[Ref.: #66794]	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
[Ref.: #66794]	4-hydroxymandelate degradation	33.33	3 of 9
[Ref.: #66794]	methane metabolism	33.33	1 of 3
[Ref.: #66794]	degradation of hexoses	33.33	6 of 18
[Ref.: #66794]	selenocysteine biosynthesis	33.33	2 of 6
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	enterobactin biosynthesis	33.33	1 of 3
[Ref.: #66794]	nitrate assimilation	33.33	3 of 9
[Ref.: #66794]	phenylpropanoid biosynthesis	30.77	4 of 13
[Ref.: #66794]	urea cycle	30.77	4 of 13
[Ref.: #66794]	4-hydroxyphenylacetate degradation	30	3 of 10
[Ref.: #66794]	mevalonate metabolism	28.57	2 of 7
[Ref.: #66794]	aclacinomycin biosynthesis	28.57	2 of 7
[Ref.: #66794]	arachidonic acid metabolism	27.78	5 of 18
[Ref.: #66794]	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
[Ref.: #66794]	metabolism of disaccharids	27.27	3 of 11
[Ref.: #66794]	d-xylose degradation	27.27	3 of 11
[Ref.: #66794]	cholesterol biosynthesis	27.27	3 of 11
[Ref.: #66794]	ascorbate metabolism	27.27	6 of 22
[Ref.: #66794]	toluene degradation	25	1 of 4
[Ref.: #66794]	catecholamine biosynthesis	25	1 of 4
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	androgen and estrogen metabolism	25	4 of 16
[Ref.: #66794]	daunorubicin biosynthesis	22.22	2 of 9
[Ref.: #66794]	degradation of pentoses	21.43	6 of 28
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15581]	Sample type/isolated from	cooling tower
[Ref.: #15581]	Country	USA
[Ref.: #15581]	Country ISO 3 Code	USA
[Ref.: #15581]	Continent	North America

[Ref.: #46478]	Sample type/isolated from	Water,industrial cooling tower
[Ref.: #46478]	Geographic location (country and/or sea, region)	Pennsylvania
[Ref.: #46478]	Country	USA
[Ref.: #46478]	Country ISO 3 Code	USA
[Ref.: #46478]	Continent	North America

[Ref.: #121375]	Sample type/isolated from	Environment, Industrial cooling tower water
[Ref.: #121375]	Geographic location (country and/or sea, region)	Pennsylvania
[Ref.: #121375]	Country	United States of America
[Ref.: #121375]	Country ISO 3 Code	USA
[Ref.: #121375]	Continent	North America
* marker position based on {}

Isolation sources categories

#Engineered

#Industrial

#Cooling tower

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15581]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #121375]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15581]	L.oakridgenesis (ATCC 33761) gene for 16S rRNA	X73397	1467	GenBank ENA	29423 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Legionella oakridgensis ATCC 33761	2545824788	complete	JGI IMG/M	1268635 tax ID	*
[Ref.: #66792]	Legionella oakridgensis ATCC 33761	GCA_001648605	scaffold	GenBank ENA	29423 tax ID	*
[Ref.: #66792]	Legionella oakridgensis NCTC11531	GCA_900636495	complete	GenBank ENA	29423 tax ID	*
[Ref.: #66792]	Legionella oakridgensis ATCC 33761 = DSM 21215	1268635.3	complete	PATRIC	1268635 tax ID	*
[Ref.: #66792]	Legionella oakridgensis ATCC 33761 = DSM 21215 strain ATCC 33761 = DSM 21215; OR-10	1268635.5	plasmid	PATRIC	1268635 tax ID	*
[Ref.: #66792]	Legionella oakridgensis RV-2-2007	1274403.3	wgs	PATRIC	1274403 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Legionella oakridgensis ATCC 33761 = DSM 21215 PATRIC: 1268635.3
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	99	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Legionella oakridgensis ATCC 33761 = DSM 21215 NCBI: GCA_000512355.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	98.985	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	96.881	yes
[Ref.: #69480]	aerobic	aerobicⓘ	no	74.982	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	88.467	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	95.445	no
[Ref.: #69480]	flagellated	motile2+ⓘ	no	81.299	no

Availability in culture collections External links

[Ref.: #15581]

Culture collection no.

DSM 21215, ATCC 33761, CCUG 16414, CIP 103884, NCTC 11531, Oak Ridge 10

[Ref.: #76213]

SI-ID 45962

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Legionella oakridgensis ATCC 33761 genome sequence and phenotypic characterization reveals its replication capacity in amoebae.	Brzuszkiewicz E, Schulz T, Rydzewski K, Daniel R, Gillmaier N, Dittmann C, Holland G, Schunder E, Lautner M, Eisenreich W, Luck C, Heuner K	Int J Med Microbiol	10.1016/j.ijmm.2013.07.003	2013	*
Phylogeny	Legionella nagasakiensis sp. nov., isolated from water samples and from a patient with pneumonia.	Yang G, Benson RF, Ratcliff RM, Brown EW, Steigerwalt AG, Thacker WL, Daneshvar MI, Morey RE, Saito A, Fields BS	Int J Syst Evol Microbiol	10.1099/ijs.0.027193-0	2011	*

References

#15581

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21215

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#34429 ; Curators of the CIP;
#46478 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 16414

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76213

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID45962.1 )

#121375 Collection of Institut Pasteur ; Curators of the CIP; CIP 103884
* These data were automatically processed and therefore are not curated

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Legionella oakridgensis ATCC 33761 = DSM 21215

Legionella oakridgensis ATCC 33761 = DSM 21215

Change proposal

Change proposal