Strain identifier

BacDive ID: 6732

Type strain: Yes

Species: Legionella anisa

Strain Designation: WA-316-C3

Strain history: CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- G.W. Gorman: strain WA-316-C3

NCBI tax ID(s): 28082 (species)

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General

@ref: 6917

BacDive-ID: 6732

DSM-Number: 17627

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, rod-shaped

description: Legionella anisa WA-316-C3 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from tap water.

NCBI tax id

  • NCBI tax id: 28082
  • Matching level: species

strain history

@refhistory
6917<- H. E. Müller, Medizinal-Untersuchungsamt, Braunschweig; <- ATCC; ATCC 35292 <- G. W. Gorman, CDC, Atlanta; WA-316-C3
67770GIFU 12075 <-- ATCC 35292 <-- G. W. Gorman WA-316-C3.
116396CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- G.W. Gorman: strain WA-316-C3

doi: 10.13145/bacdive6732.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella anisa
  • full scientific name: Legionella anisa Gorman et al. 1985

@ref: 6917

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella anisa

full scientific name: Legionella anisa Gorman et al. 1985

strain designation: WA-316-C3

type strain: yes

Morphology

cell morphology

  • @ref: 116396
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 6917
  • incubation period: 3-7 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6917BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
41839MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
116396CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23
116396CIP Medium 748yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=748

culture temp

@refgrowthtypetemperaturerange
6917positivegrowth37mesophilic
41839positivegrowth37mesophilic
49701positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
6917microaerophile
49701microaerophile

observation

  • @ref: 67770
  • observation: quinones: Q-10

enzymes

@refvalueactivityec
6917catalase+1.11.1.6
6917cytochrome-c oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
116396oxidase-
116396catalase+1.11.1.6

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
6917-+++/--+-+/---++--------
6917-++--+----+/-+--------
116396-++--+----++--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudesampling date
6917tap waterLos AngelesUSAUSANorth America34.0522-118.244
49701Tap water1981
67770Tap waterLos Angeles, CAUSAUSANorth America
116396Environment, Tap waterLos Angeles, CaliforniaUnited States of AmericaUSANorth America

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Freshwater
#Engineered#Built environment#Water reservoir (Aquarium/pool)
#Engineered#Built environment

taxonmaps

  • @ref: 69479
  • File name: preview.99_1173.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_70;97_584;98_923;99_1173&stattab=map
  • Last taxonomy: Legionella anisa
  • 16S sequence: Z32635
  • Sequence Identity:
  • Total samples: 413
  • soil counts: 154
  • aquatic counts: 179
  • animal counts: 45
  • plant counts: 35

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
69172Risk group (German classification)
1163962Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218L.anisa (ATCC 35292) gene for 16S rRNAX733941467ena28082
20218L.anisa (WA-316-C3) gene for small subunit ribosomal RNAZ326351398ena28082
6917Legionella anisa 16S ribosomal RNA gene, partial sequenceAY7447761486ena28082

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella anisa WA-316-C3GCA_001467525scaffoldncbi28082
66792Legionella anisa WA-316-C3GCA_900639785scaffoldncbi28082
66792Legionella anisa strain FDAARGOS_148028082.25completepatric28082
66792Legionella anisa strain WA-316-C328082.12wgspatric28082
66792Legionella anisa WA-316-C32663763523draftimg28082
66792Legionella anisa strain FDAARGOS_148028082.27completepatric28082
66792Legionella anisa strain FDAARGOS_148028082.32completepatric28082
66792Legionella anisa strain FDAARGOS_148028082.26completepatric28082

GC content

  • @ref: 67770
  • GC-content: 42
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes85.908no
gram-positiveno98.41no
anaerobicno98.63yes
halophileno87.192no
spore-formingno92.615no
glucose-utilyes69.191no
flagellatedno76.609no
aerobicno89.796yes
thermophileno99.207yes
glucose-fermentno87.754no

External links

@ref: 6917

culture collection no.: DSM 17627, ATCC 35292, CIP 103870, NCTC 11974, WDCM 00106, CCUG 29665, JCM 7573, KCTC 12010, CECT 8177

straininfo link

  • @ref: 76202
  • straininfo: 40977

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny2405005Characterization of a Legionella anisa strain isolated from a patient with pneumonia.Thacker WL, Benson RF, Hawes L, Mayberry WR, Brenner DJJ Clin Microbiol10.1128/jcm.28.1.122-123.19901990Aged, Bacteriological Techniques, Bronchoalveolar Lavage Fluid/microbiology, Fatty Acids/analysis, Female, Humans, Legionella/analysis/classification/*isolation & purification, Legionellosis/*microbiology, Pneumonia/*microbiologyPathogenicity
Phylogeny3985609Legionella anisa: a new species of Legionella isolated from potable waters and a cooling tower.Gorman GW, Feeley JC, Steigerwalt A, Edelstein PH, Moss CW, Brenner DJAppl Environ Microbiol10.1128/aem.49.2.305-309.19851985Air Conditioning, California, Chicago, Fatty Acids/analysis, Legionella/analysis/classification/*isolation & purification/physiology, New York, Phenotype, Serotyping, Terminology as Topic, Ubiquinone/analysis, *Water Microbiology, Water SupplyPhenotype

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6917Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17627)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17627
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41839Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15715
49701Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 29665)https://www.ccug.se/strain?id=29665
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76202Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID40977.1StrainInfo: A central database for resolving microbial strain identifiers
116396Curators of the CIPCollection of Institut Pasteur (CIP 103870)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103870