Strain identifier

BacDive ID: 6657

Type strain: Yes

Species: Liquorilactobacillus ghanensis

Strain Designation: L489

Strain history: CIP <- 2007, CCUG <- D.S. Nielsen, Copenhagen Univ., Copenhagen, Denmark: strain L489

NCBI tax ID(s): 1423750 (strain), 399370 (species)

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General

@ref: 7629

BacDive-ID: 6657

DSM-Number: 18630

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, motile, rod-shaped

description: Liquorilactobacillus ghanensis L489 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from cocoa fermentation.

NCBI tax id

NCBI tax idMatching level
1423750strain
399370species

strain history

@refhistory
7629<- D.S. Nielsen, L489
67770CCUG 53453 <-- D. Nielsen L489.
119611CIP <- 2007, CCUG <- D.S. Nielsen, Copenhagen Univ., Copenhagen, Denmark: strain L489

doi: 10.13145/bacdive6657.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Liquorilactobacillus
  • species: Liquorilactobacillus ghanensis
  • full scientific name: Liquorilactobacillus ghanensis (Nielsen et al. 2007) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus ghanensis

@ref: 7629

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Liquorilactobacillus

species: Liquorilactobacillus ghanensis

full scientific name: Liquorilactobacillus ghanensis (Nielsen et al. 2007) Zheng et al. 2020

strain designation: L489

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
32012positive1.95 µm0.75 µmrod-shapedyes
69480positive100
119611positiverod-shapedyes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7629PEDIOCOCCUS DAMNOSUS MEDIUM (DSMZ Medium 231)yeshttps://mediadive.dsmz.de/medium/231Name: PEDIOCOCCUS DAMNOSUS MEDIUM (DSMZ Medium 231) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Yeast extract 5.0 g/l Na-acetate 5.0 g/l K2HPO4 2.0 g/l (NH4)3 citrate 2.0 g/l Tween 80 1.0 g/l Cysteine hydrochloride 0.5 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
37651MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
119611CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
7629positivegrowth30mesophilic
32012positivegrowth15-45
32012positiveoptimum45thermophilic
37651positivegrowth37mesophilic
59904positivegrowth30-37mesophilic
67770positivegrowth37mesophilic
119611positivegrowth15-45
119611nogrowth10psychrophilic

culture pH

  • @ref: 32012
  • ability: positive
  • type: optimum
  • pH: 3.9
  • PH range: acidophile

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
7629microaerophile
32012anaerobe
59904anaerobe
119611facultative anaerobe

spore formation

@refspore formationconfidence
32012no
69481no100
69480no99.988

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3201217057cellobiose+carbon source
3201228757fructose+carbon source
3201228260galactose+carbon source
3201217234glucose+carbon source
3201217754glycerol+carbon source
3201224996lactate+carbon source
3201217716lactose+carbon source
3201217306maltose+carbon source
3201229864mannitol+carbon source
3201237684mannose+carbon source
32012506227N-acetylglucosamine+carbon source
3201226546rhamnose+carbon source
3201217814salicin+carbon source
3201230911sorbitol+carbon source
3201217992sucrose+carbon source
3201227082trehalose+carbon source
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose+builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837127613amygdalin+builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose+builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
11961117632nitrate-reduction
11961116301nitrite-reduction
11961117632nitrate+respiration

metabolite tests

  • @ref: 119611
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
119611oxidase-
119611alcohol dehydrogenase-1.1.1.1
119611catalase-1.11.1.6
119611lysine decarboxylase-4.1.1.18
119611ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119611-----+----++---++---

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
7629----------++++-+--+---++-+++---++------+----------
119611---------++++-+--+---++++++---++------+----------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
7629cocoa fermentationNew TafoGhanaGHAAfrica
59904Cocoa fermentationGhanaGHAAfrica
67770Ghanaian cocoa fermentations
119611Cocoa fermentationTafoGhanaGHAAfrica

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production
#Host Body-Site#Plant#Fruit (Seed)

taxonmaps

  • @ref: 69479
  • File name: preview.99_7552.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_2680;97_3290;98_4140;99_7552&stattab=map
  • Last taxonomy: Liquorilactobacillus ghanensis
  • 16S sequence: LC480807
  • Sequence Identity:
  • Total samples: 3634
  • soil counts: 60
  • aquatic counts: 136
  • animal counts: 3202
  • plant counts: 236

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
76291Risk group (German classification)
1196111Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
7629Lactobacillus ghanensis strain L489 16S ribosomal RNA gene, partial sequenceDQ5234891460ena399370
67770Lactobacillus ghanensis JCM 15611 gene for 16S ribosomal RNA, partial sequenceLC4808071496ena399370

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus ghanensis DSM 186301423750.3wgspatric1423750
66792Liquorilactobacillus ghanensis DSM 186302663762875draftimg1423750
67770Liquorilactobacillus ghanensis DSM 18630GCA_001435235scaffoldncbi1423750

GC content

@refGC-contentmethod
762937.8
6777037.1genome sequence analysis
6777037.8thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes66.405yes
gram-positiveyes94.045no
anaerobicno72.527no
halophileno50.594no
spore-formingno89.908yes
glucose-utilyes90.847yes
thermophileno96.857no
aerobicno96.758yes
flagellatedno86.086no
glucose-fermentyes90.203no

External links

@ref: 7629

culture collection no.: DSM 18630, CCUG 53453, CIP 109616, JCM 15611

straininfo link

  • @ref: 76131
  • straininfo: 309102

literature

  • topic: Phylogeny
  • Pubmed-ID: 17625177
  • title: Lactobacillus ghanensis sp. nov., a motile lactic acid bacterium isolated from Ghanaian cocoa fermentations.
  • authors: Nielsen DS, Schillinger U, Franz CMAP, Bresciani J, Amoa-Awua W, Holzapfel WH, Jakobsen M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.64811-0
  • year: 2007
  • mesh: Ammonia/metabolism, Arginine/metabolism, Cacao/*microbiology, Carbohydrate Metabolism, Catalase/analysis, Cell Wall, Cluster Analysis, DNA Fingerprinting, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fermentation, Genes, rRNA, Ghana, Glucose/metabolism, Lactic Acid/metabolism, Lactobacillus/chemistry/*classification/*isolation & purification, Locomotion, Microscopy, Electron, Scanning, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/analysis, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
7629Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18630)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18630
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32012Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2826528776041
37651Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7379
59904Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 53453)https://www.ccug.se/strain?id=53453
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76131Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID309102.1StrainInfo: A central database for resolving microbial strain identifiers
119611Curators of the CIPCollection of Institut Pasteur (CIP 109616)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109616