Strain identifier

BacDive ID: 6519

Type strain: Yes

Species: Fructilactobacillus sanfranciscensis

Strain Designation: L-12

Strain history: CIP <- 1988, DSM <- ATCC <- L. Kline: strain L-12

NCBI tax ID(s): 1423800 (strain), 1625 (species)

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General

@ref: 8823

BacDive-ID: 6519

DSM-Number: 20451

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped

description: Fructilactobacillus sanfranciscensis L-12 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from San Francisco sourdough.

NCBI tax id

NCBI tax idMatching level
1625species
1423800strain

strain history

@refhistory
8823<- ATCC <- L. Kline, L-12
67770DSM 20451 <-- ATCC 27651 <-- L. Kline L-12.
122417CIP <- 1988, DSM <- ATCC <- L. Kline: strain L-12

doi: 10.13145/bacdive6519.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Fructilactobacillus
  • species: Fructilactobacillus sanfranciscensis
  • full scientific name: Fructilactobacillus sanfranciscensis (Weiss and Schillinger 1984 ex Kline and Sugihara 1971) Zheng et al. 2020

  • synonyms

    @refsynonym
    20215Lactobacillus sanfrancisco
    20215Lactobacillus sanfranciscensis

@ref: 8823

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Fructilactobacillus

species: Fructilactobacillus sanfranciscensis

full scientific name: Fructilactobacillus sanfranciscensis (Weiss and Schillinger 1984) Zheng et al. 2020

strain designation: L-12

type strain: yes

Morphology

cell morphology

  • @ref: 122417
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8823SOUR DOUGH MEDIUM (DSMZ Medium 225)yeshttps://mediadive.dsmz.de/medium/225Name: SOUR DOUGH MEDIUM (DSMZ Medium 225) Composition: Maltose 20.0 g/l Casein peptone 6.0 g/l Tween 80 0.3 g/l Yeast extract Distilled water
35517MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
122417CIP Medium 41yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=41
122417CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
8823positivegrowth30mesophilic
35517positivegrowth37mesophilic
49936positivegrowth30mesophilic
67770positivegrowth30mesophilic
122417positivegrowth15-37
122417nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8823anaerobe
8823microaerophile
122417facultative anaerobe

murein

  • @ref: 8823
  • murein short key: A11.04
  • type: A3alpha L-Lys-L-Ala

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
122417nitrate-reduction17632
122417nitrite-reduction16301

antibiotic resistance

  • @ref: 122417
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 122417
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 122417
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
122417oxidase-
122417alcohol dehydrogenase-1.1.1.1
122417catalase-1.11.1.6
122417lysine decarboxylase-4.1.1.18
122417ornithine decarboxylase-4.1.1.17
122417urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122417-+---++---++--------

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8823----------------------------+---------------------
8823-----------+----------------+---------------------
8823-----------+----------------+---------------------
122417----------+----------------+---------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
8823San Francisco sourdough
49936San Francisco sour dough
67770San Francisco sourdough
122417Food, San Francisco sour doughUnited States of AmericaUSANorth America

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Food

taxonmaps

  • @ref: 69479
  • File name: preview.99_3446.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_1300;97_2156;98_2645;99_3446&stattab=map
  • Last taxonomy: Fructilactobacillus sanfranciscensis subclade
  • 16S sequence: LC483557
  • Sequence Identity:
  • Total samples: 11904
  • soil counts: 658
  • aquatic counts: 1310
  • animal counts: 9668
  • plant counts: 268

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
88231Risk group (German classification)
1224171Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus sanfrancisco 16S ribosomal RNAM588301528ena1625
8823L.sanfrancisco (ATCC 27651 T) 16S rRNA geneX763271572ena1625
67770Lactobacillus sanfranciscensis gene for 16S ribosomal RNA, partial sequenceLC4835571478ena1625
67770L.sanfrancisco 16S rRNA geneX611321515ena1625

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Fructilactobacillus sanfranciscensis JCM 5668GCA_003369815contigncbi1625
66792Fructilactobacillus sanfranciscensis DSM 20451 TMW 1.53GCA_002907365contigncbi1423800
66792Lactobacillus sanfranciscensis DSM 204511423800.3wgspatric1423800
66792Lactobacillus sanfranciscensis DSM 20451 strain TMW 1.531423800.4wgspatric1423800
66792Lactobacillus sanfranciscensis strain JCM 56681625.37wgspatric1625
66792Fructilactobacillus sanfranciscensis DSM 204512667527754draftimg1423800
67770Fructilactobacillus sanfranciscensis DSM 20451GCA_001436035scaffoldncbi1423800

GC content

@refGC-contentmethod
882338.7
6777034.7genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno96.806no
gram-positiveyes95.504no
anaerobicno85.254no
halophileyes94.001no
spore-formingno97.216no
glucose-utilyes87.635no
thermophileno98.856no
aerobicno95.059yes
flagellatedno98.867no
glucose-fermentyes88.499no

External links

@ref: 8823

culture collection no.: DSM 20451, ATCC 27651, NRRL B-3934, CCUG 30143, LMG 7946, JCM 5668, BCRC 14063, CIP 103252, KCTC 3205, LMG 16002, LMG 17498, NRIC 1548, NRRL: B-3934

straininfo link

  • @ref: 75991
  • straininfo: 3367

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology9851037Maltose metabolism of Lactobacillus sanfranciscensis: cloning and heterologous expression of the key enzymes, maltose phosphorylase and phosphoglucomutase.Ehrmann MA, Vogel RFFEMS Microbiol Lett10.1111/j.1574-6968.1998.tb13302.x1998Amino Acid Sequence, Consensus Sequence, Escherichia coli/genetics, Genes, Bacterial, Genomic Library, Glucosyltransferases/biosynthesis/*genetics, Lactobacillus/enzymology/*genetics, Molecular Sequence Data, Phosphoglucomutase/biosynthesis/*genetics, Recombinant Proteins/biosynthesis, Restriction Mapping, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Substrate SpecificityGenetics
Genetics11822682Characterisation of IS153, an IS3-family insertion sequence isolated from Lactobacillus sanfranciscensis and its use for strain differentiation.Ehrmann MA, Vogel RESyst Appl Microbiol10.1078/0723-2020-000572001Amino Acid Sequence, Base Sequence, *DNA Transposable Elements, Gene Dosage, Lactobacillus/classification/*genetics, Molecular Sequence Data, Polymerase Chain ReactionPhylogeny
Biotechnology15287876Barotolerance is inducible by preincubation under hydrostatic pressure, cold-, osmotic- and acid-stress conditions in Lactobacillus sanfranciscensis DSM 20451T.Scheyhing CH, Hormann S, Ehrmann MA, Vogel RFLett Appl Microbiol10.1111/j.1472-765X.2004.01578.x2004Cold Temperature, Food Microbiology, Food Preservation/methods, *Heat-Shock Response, Hydrogen-Ion Concentration, *Hydrostatic Pressure, Lactobacillus/*growth & development/physiology, Osmotic PressureStress
Phylogeny15836738Biodiversity of Lactobacillus sanfranciscensis strains isolated from five sourdoughs.Kitahara M, Sakata S, Benno YLett Appl Microbiol10.1111/j.1472-765X.2005.01678.x2005Fermentation, *Food Industry, Lactobacillus/*genetics/isolation & purification, Ribotyping, Species Specificity, Triticum/metabolism/*microbiologyEnzymology
Metabolism16204547Functional characterization of the proteolytic system of Lactobacillus sanfranciscensis DSM 20451T during growth in sourdough.Vermeulen N, Pavlovic M, Ehrmann MA, Ganzle MG, Vogel RFAppl Environ Microbiol10.1128/AEM.71.10.6260-6266.20052005Bacterial Proteins/*genetics/metabolism, Bread/*microbiology, Fermentation, Gene Expression Regulation, Bacterial, Hydrolysis, Lactobacillus/enzymology/genetics/*growth & development, Membrane Transport Proteins/genetics/metabolism, Molecular Sequence Data, Peptide Hydrolases/*genetics/metabolism, Sequence Analysis, DNAGenetics
Proteome16470640Comparative proteome approach to characterize the high-pressure stress response of Lactobacillus sanfranciscensis DSM 20451(T).Hormann S, Scheyhing C, Behr J, Pavlovic M, Ehrmann M, Vogel RFProteomics10.1002/pmic.2004020862006Amino Acid Sequence, Chromatography, Liquid, Electrophoresis, Gel, Two-Dimensional, *Heat-Shock Response, Hydrostatic Pressure, Image Processing, Computer-Assisted, Lactobacillus/*drug effects/genetics/*growth & development, Mass Spectrometry, Molecular Sequence Data, Peptide Mapping, Proteome/*analysis, Sequence Analysis, Protein, Silver Staining, Sodium Chloride/*pharmacology, Spectrometry, Mass, Electrospray Ionization, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Temperature, Trypsin/pharmacologyEnzymology
Metabolism16719504Influence of peptide supply and cosubstrates on phenylalanine metabolism of Lactobacillus sanfranciscensis DSM20451(T) and Lactobacillus plantarum TMW1.468.Vermeulen N, Ganzle MG, Vogel RFJ Agric Food Chem10.1021/jf052733e2006Amino Acids, Branched-Chain/pharmacology, Bread/analysis, Fermentation, Ketoglutaric Acids/pharmacology, Lactobacillus/*metabolism, Lactobacillus plantarum/*metabolism, Peptides/analysis/*metabolism, Phenylacetates/*metabolism, Phenylalanine/analysis/*metabolism
Metabolism17496130Glutathione reductase from Lactobacillus sanfranciscensis DSM20451T: contribution to oxygen tolerance and thiol exchange reactions in wheat sourdoughs.Jansch A, Korakli M, Vogel RF, Ganzle MGAppl Environ Microbiol10.1128/AEM.02322-062007Aerobiosis, Anaerobiosis, Anti-Bacterial Agents/metabolism/pharmacology, Biological Transport, Cysteine/metabolism, Cystine/metabolism, DNA, Bacterial/chemistry/genetics, Fermentation, Flour/analysis/*microbiology, Glutathione Reductase/*genetics/*physiology, Lactobacillus/*enzymology/growth & development/*physiology, Molecular Sequence Data, Mutagenesis, Insertional, Oxygen/*metabolism/pharmacology, Paraquat/pharmacology, Sequence Analysis, DNA, Sulfhydryl Compounds/*metabolism, *Triticum/metabolismEnzymology
Metabolism20208023Glutathione protects Lactobacillus sanfranciscensis against freeze-thawing, freeze-drying, and cold treatment.Zhang J, Du GC, Zhang Y, Liao XY, Wang M, Li Y, Chen JAppl Environ Microbiol10.1128/AEM.00026-092010*Cold Temperature, Fatty Acids/metabolism, Fermentation, Freeze Drying, Freezing, Glutathione/*metabolism, Lactobacillus/*metabolism/physiology, Lipid Peroxidation
Metabolism21056772Contribution of the NADH-oxidase (Nox) to the aerobic life of Lactobacillus sanfranciscensis DSM20451T.Jansch A, Freiding S, Behr J, Vogel RFFood Microbiol10.1016/j.fm.2010.08.0012010Bread/microbiology, Colony Count, Microbial, Culture Media, Conditioned/metabolism, *Fermentation, Gene Expression Regulation, Bacterial, Genes, Bacterial, Hydrogen-Ion Concentration, Lactic Acid/metabolism, Lactobacillus/*enzymology/*genetics, Manganese/metabolism, Multienzyme Complexes/*metabolism, NADH, NADPH Oxidoreductases/*metabolism, Oxygen/metabolism, Paraquat/metabolismCultivation
Metabolism22608235Glutathione improves the cold resistance of Lactobacillus sanfranciscensis by physiological regulation.Zhang J, Li Y, Chen W, Du GC, Chen JFood Microbiol10.1016/j.fm.2012.04.0062012Bacterial Proteins/genetics/metabolism, Cold Temperature, Glutathione/*metabolism, Glyceraldehyde-3-Phosphate Dehydrogenases/genetics/metabolism, L-Lactate Dehydrogenase/genetics/metabolism, Lactobacillus/enzymology/genetics/*physiology, Pyruvate Kinase/genetics/metabolism, Stress, PhysiologicalEnzymology
Metabolism24795368The transcriptional response of Lactobacillus sanfranciscensis DSM 20451T and its tcyB mutant lacking a functional cystine transporter to diamide stress.Stetina M, Behr J, Vogel RFAppl Environ Microbiol10.1128/AEM.00367-142014Amino Acid Transport Systems/*genetics, Cystine/metabolism, DNA, Bacterial/genetics, Diamide/metabolism, Genes, Bacterial, Heat-Shock Response, Lactobacillus/*genetics/metabolism, Mutation, Oxidative Stress/*genetics, RNA, Bacterial/genetics, Sequence Analysis, RNA, *Transcription, GeneticTranscriptome
Phylogeny26297032Lactobacillus vespulae sp. nov., isolated from gut of a queen wasp (Vespula vulgaris).Hoang VA, Kim YJ, Nguyen NL, Kim SK, Yang DCInt J Syst Evol Microbiol10.1099/ijsem.0.0004202015Animals, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Fatty Acids/chemistry, Female, Gastrointestinal Tract/*microbiology, Lactobacillus/*classification/genetics/isolation & purification, Molecular Sequence Data, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Wasps/*microbiologyGenetics
Phylogeny27902183Lactobacillus ixorae sp. nov., isolated from a flower (West-Indian jasmine).Techo S, Miyashita M, Shibata C, Tanaka N, Wisetkhan P, Visessanguan W, Tanasupawat SInt J Syst Evol Microbiol10.1099/ijsem.0.0015472016Bacillus coagulans, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fermentation, Flowers/*microbiology, Jasminum/*microbiology, Lactic Acid/biosynthesis, Lactobacillus/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, ThailandTranscriptome
Metabolism30175916Effect of Glutathione Dehydrogenase of Lactobacillus sanfranciscensis on Gluten Properties and Bread Volume in Type I Wheat Sourdough Bread.Xu D, Tang K, Hu Y, Xu X, Ganzle MGJ Agric Food Chem10.1021/acs.jafc.8b032982018Bacterial Proteins/*metabolism, Bread/*analysis, Fermentation, Glutathione Reductase/*metabolism, Glutens/*chemistry/metabolism, Lactobacillus/*enzymology/metabolism, Peroxides/analysis/metabolism, Saccharomyces cerevisiae/metabolism, Sulfhydryl Compounds/analysis/metabolism, Triticum/chemistry/metabolism/*microbiologyEnzymology
31552010Effect of Mixed Cultures of Yeast and Lactobacilli on the Quality of Wheat Sourdough Bread.Xu D, Zhang Y, Tang K, Hu Y, Xu X, Ganzle MGFront Microbiol10.3389/fmicb.2019.021132019
Metabolism33562539Degradation of Wheat Germ Agglutinin during Sourdough Fermentation.Tovar LER, Ganzle MGFoods10.3390/foods100203402021

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8823Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20451)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20451
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
35517Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15029
49936Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 30143)https://www.ccug.se/strain?id=30143
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
75991Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3367.1StrainInfo: A central database for resolving microbial strain identifiers
122417Curators of the CIPCollection of Institut Pasteur (CIP 103252)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103252