Strain identifier

BacDive ID: 6474

Type strain: No

Species: Fructilactobacillus fructivorans

Strain Designation: H42

Strain history: CIP <- 1988, DSMZ, Lactobacillus homohiochii <- ATCC <- K. Kitahara: strain H42 <- T. Kaneko

NCBI tax ID(s): 1614 (species)

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General

@ref: 8921

BacDive-ID: 6474

DSM-Number: 20571

keywords: genome sequence, 16S sequence, Bacteria, anaerobe

description: Fructilactobacillus fructivorans H42 is an anaerobe bacterium that was isolated from spoilt sake.

NCBI tax id

  • NCBI tax id: 1614
  • Matching level: species

strain history

@refhistory
8921<- ATCC <- IAM (K. Kitahara), H42 <- T. Kaneko
67770ATCC 15434 <-- K. Kitahara H42 <-- T. Kaneko.
119470CIP <- 1988, DSMZ, Lactobacillus homohiochii <- ATCC <- K. Kitahara: strain H42 <- T. Kaneko

doi: 10.13145/bacdive6474.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Fructilactobacillus
  • species: Fructilactobacillus fructivorans
  • full scientific name: Fructilactobacillus fructivorans (Charlton et al. 1934) Zheng et al. 2020

  • synonyms

    @refsynonym
    20215Lactobacillus trichodes
    20215Lactobacillus heterohiochii
    20215Lactobacillus homohiochii
    20215Lactobacillus fructivorans

@ref: 8921

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Fructilactobacillus

species: Fructilactobacillus fructivorans

full scientific name: Fructilactobacillus fructivorans (Charlton et al. 1934) Zheng et al. 2020

strain designation: H42

type strain: no

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
119470positiverod-shapedno
69480positive91.51

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8921LACTOBACILLUS MEDIUM II (DSMZ Medium 93)yeshttps://mediadive.dsmz.de/medium/93Name: LACTOBACILLUS MEDIUM II (DSMZ Medium 93) Composition: Na-acetate 20.0 g/l Glucose 20.0 g/l Agar 15.0 g/l Casein peptone 10.0 g/l Yeast extract 5.0 g/l Meat extract 2.0 g/l K2HPO4 0.5 g/l KH2PO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l DL-mevalonic acid 0.03 g/l FeSO4 x 7 H2O 0.01 g/l MnSO4 x H2O 0.0075 g/l Tween 80 Ethanol Distilled water
42101MEDIUM 46 - for Lactobacillus homohiochiiyesDistilled water make up to (1000.000 ml);Iron (II) sulphate heptahydrate (10.000 mg);Magnesium sulphate heptahydrate (200.000 mg);Agar (15.000 g);Glucose (20.000 g);Yeast extract (5.000 g);Potassium di-hydrogen phosphate (0.500 g);Sodium acetate (20.000g)
119470CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperature
8921positivegrowth28
42101positivegrowth25
50928positivegrowth30-37
67770positivegrowth30

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
50928anaerobe
50928microaerophile
119470facultative anaerobe

spore formation

  • @ref: 69481
  • spore formation: no
  • confidence: 100

murein

  • @ref: 8921
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

observation

  • @ref: 67770
  • observation: Assay of Mevalonic acid

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin+builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
119470nitrate-reduction17632
119470nitrite-reduction16301
119470nitrate+respiration17632

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
119470oxidase-
119470alcohol dehydrogenase-1.1.1.1
119470catalase-1.11.1.6
119470lysine decarboxylase-4.1.1.18
119470ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119470-+---++---+---------

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8921-----------++------------+------------------------

Isolation, sampling and environmental information

isolation

@refsample type
8921spoilt sake
50928Sake,spoiled
67770Spoiled sake
119470Food, Spoiled sake

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Fermented
#Environmental#Microbial community
#Engineered#Food production#Beverage

taxonmaps

  • @ref: 69479
  • File name: preview.99_3338.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_1742;97_2083;98_2559;99_3338&stattab=map
  • Last taxonomy: Fructilactobacillus fructivorans subclade
  • 16S sequence: LC311740
  • Sequence Identity:
  • Total samples: 639
  • soil counts: 41
  • aquatic counts: 96
  • animal counts: 438
  • plant counts: 64

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
89211Risk group (German classification)
1194701Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus homohiochii ATCC 15434 16S ribosomal RNA gene, partial sequenceAF429511503nuccore33961
20218Lactobacillus homohiochii strain ATCC 15434 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF429598537nuccore33961
20218Lactobacillus homohiochii partial 16S rRNA gene, strain type strain:DSM 20571AM1137801537nuccore33961
20218Fructilactobacillus fructivorans JCM 1199 gene for 16S rRNA, partial sequenceAB289159639nuccore33961
20218Fructilactobacillus fructivorans gene for 16S ribosomal RNA, partial sequenceD31694206nuccore33961
20218Fructilactobacillus fructivorans JCM 7793 gene for 16S rRNA, partial sequenceAB289161645nuccore33961
20218Lactobacillus homohiochii partial 16S rRNA gene, strain LMG 9478TAJ6215521473nuccore33961
20218Fructilactobacillus fructivorans NBRC 15887 gene for 16S rRNA, partial sequenceAB6809901500nuccore33961
67770Lactobacillus homohiochii gene for 16S ribosomal RNA, partial sequence, strain: JCM 1199LC3117391506nuccore1614
67770Lactobacillus homohiochii gene for 16S ribosomal RNA, partial sequence, strain: JCM 7793LC3117401506nuccore1614

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus homohiochii strain DSM 2057133961.3wgspatric1614
66792Lactobacillus homohiochii DSM 205712657245150draftimg33961
67770Fructilactobacillus fructivorans DSM 20571GCA_001436985scaffoldncbi1614

GC content

@refGC-contentmethod
892138.0
6777035.4thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesno100no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes91.51no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no76.951no
69480spore-formingspore-formingAbility to form endo- or exosporesno88.777no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no92.05yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno93.267no
69480flagellatedmotile2+Ability to perform flagellated movementno88.5no

External links

@ref: 8921

culture collection no.: CCUG 32247, LMG 9478, IAM H42, CIP 103141, LMG 11720, DSM 20571, ATCC 15434, JCM 1199, BCRC 12938, CECT 4136, IFO 15887, JCM 7793, KCTC 3534, NBRC 15887, NCFB 2719, NCIMB 702719, NRIC 0974, NCDO 2719

straininfo link

  • @ref: 75947
  • straininfo: 92310

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8921Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20571)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20571
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
42101Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14906
50928Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 32247)https://www.ccug.se/strain?id=32247
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
75947Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92310.1StrainInfo: A central database for resolving microbial strain identifiers
119470Curators of the CIPCollection of Institut Pasteur (CIP 103141)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103141